Flag-M4-AR-W741C-S81A
(Plasmid
#171248)
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PurposeMammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser81Ala (AR-W741C-S81A)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 171248 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep3XFLAG-CMV-10
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Backbone manufacturerSigma-Aldrich
- Backbone size w/o insert (bp) 6400
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name3xFlag-tagged human androgen receptor Trp741Cys-Ser81Ala mutant
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Alt nameFlag-AR-W741C-S81A
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SpeciesH. sapiens (human)
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Insert Size (bp)3000
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MutationAR-Trp741Cys-Ser81Ala (AR-W741C-S81A)
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Entrez GeneAR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
- Promoter CMV
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Tag
/ Fusion Protein
- Flag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer CMV_fwd_primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Flag-tagged human AR Ser-Pro phosphorylation mutant that binds to bicalutamide, and the exact amino position and composition may slightly vary based on human AR versions.Two XmaI sites on the vector backbone and one EcoRI site at the 3'UTR of AR cDNA were deleted by DNA mutagenesis.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Flag-M4-AR-W741C-S81A was a gift from Steven Balk (Addgene plasmid # 171248 ; http://n2t.net/addgene:171248 ; RRID:Addgene_171248) -
For your References section:
Androgen receptor serine 81 phosphorylation mediates chromatin binding and transcriptional activation. Chen S, Gulla S, Cai C, Balk SP. J Biol Chem. 2012 Mar 9;287(11):8571-83. doi: 10.1074/jbc.M111.325290. Epub 2012 Jan 24. 10.1074/jbc.M111.325290 PubMed 22275373