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Showing: 11951 - 12000 of 28583 results

  1. TfR-moxGFP-DHFR

    Plasmid
    #173012
    Purpose
    For light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter. Fused to oxidation resistant version of GFP (moxGFP).
    Depositor
    Matthew Kennedy
    Article
    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212
    Insert
    TfR-moxGFP-DHFR
    Use
    Tags
    DHFR and moxGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    173012-plasmid-map-sequence-id-339204

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  14. pAAV-hSYN-bArrestin2-TevC-P2A-TdTomato-WPRE-bGHpA

    Plasmid
    #89873
    Purpose
    expresses iTango bArrestin2-TevC component and TdTomato bicistronically for AAV production
    Depositor
    Hyungbae Kwon
    Article
    Lee et al Nat Methods. 2017 May;14(5):495-503. doi
    Insert
    bArrestin2-TevC-P2A-TdTomato
    Use
    AAV
    Tags
    TdTomato
    Expression
    Mammalian
    Mutation
    R173H in TdTomato (Please see depositor comments …
    Promoter
    Human synapsin
    Available since
    May 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    89873-plasmid-map-sequence-id-177108

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  15. pDO001

    Plasmid
    #198828
    Purpose
    Inhibition of synaptic vessel release due to disruption of synaptic vessel function by reactive oxygen species production.
    Depositor
    Paul Sternberg
    Article
    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12
    Insert
    15xUAS::miniSOG-103L-VAMP2-mCherry::let-858 3'UTR
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    April 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    198828-plasmid-map-sequence-id-390248

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  20. pYL236

    Plasmid
    #173184
    Purpose
    CRISPR donor plasmid for making the WT EZH2 control cell line. It contains a puromycin resistance gene and an mCherry gene
    Depositor
    Thomas Cech
    Article
    Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:
    Insert
    EZH2 (EZH2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    173184-plasmid-map-sequence-id-341220

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  22. pAAV_CAG_FLEX_PKIαmut-P2A-mRuby3_WPRE

    Plasmid
    #196678
    Purpose
    AAV plasmid for mammalian Cre-dependent expression of PKIαmut-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIαmut bears the R20G/R21G mutation that renders it non-functional.
    Depositor
    Haining Zhong
    Article
    Ma et al Nature. 2022 Nov;611(7937):762-768. doi:
    Insert
    PKIαmut-P2A-mRuby3
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    196678-plasmid-map-sequence-id-385123

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  25. pFRLuc-CoV2-delStem1

    Plasmid
    #177616
    Purpose
    Dual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. 3-nucleotide deletion in Stem 1 (negative control).
    Depositor
    Junjie Guo
    Article
    Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11
    Insert
    SARS-CoV-2 FSE
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    3-nucleotide (UAC) deletion in Stem 1
    Promoter
    Available since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    177616-plasmid-map-sequence-id-350042

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  29. pAAV-shNCLX-2

    Plasmid
    #181871
    Purpose
    Expresses NCLX-targeted shRNA under control of the U6 promoter and mCherry under control of the CaMKIIa promoter
    Depositor
    Hilmar Bading
    Article
    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10
    Insert
    solute carrier 8 family member B1 (Slc8b1 Mouse)
    Use
    AAV, Adenoviral, and Mouse Targeting
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181871-plasmid-map-sequence-id-355961

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  30. pAAV-shNCLX-1

    Plasmid
    #181870
    Purpose
    Expresses NCLX-targeted shRNA under control of the U6 promoter and mCherry under control of the CaMKIIa promoter
    Depositor
    Hilmar Bading
    Article
    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10
    Insert
    solute carrier 8 family member B1 (Slc8b1 Mouse)
    Use
    AAV, Adenoviral, and Mouse Targeting
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181870-plasmid-map-sequence-id-355957

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  37. attB53_SNCB+_attB53

    Plasmid
    #183614
    Purpose
    RMCE vector to shuttle puromycin resistance, anti-GFP SynNotch receptor, tagBFP and TRE-mCherry cassettes into attP50-flanked landing pad (Addgene 183609). All cassettes on sense (+) strand.
    Depositor
    Sally Lowell
    Article
    Malaguti et al Development. 2022 May 26. pii: 275525. d
    Inserts
    LaG17 GFP nanobody SynNotch tTA
    TRE-mCherry-SV40pA
    tagBFP
    Use
    Rmce
    Tags
    Myc and human CD8a signal sequence
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    183614-plasmid-map-sequence-id-361603

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  42. pBetaActin-KIF13aMotor-mScarlet-FKBP

    Plasmid
    #191337
    Purpose
    Expresses the fluorescently labeled plus-end directed KIF13a motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB
    Depositor
    Frank Bradke
    Article
    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:
    Insert
    KIF13aMotor-mScarlet-FKBP (KIF13A Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    motor domain aa 1-639
    Promoter
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    191337-plasmid-map-sequence-id-381769

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  45. pAAV-Hb9-LMO3 (sbGLuc-VChR1-EYFP)

    Plasmid
    #114103
    Purpose
    fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics
    Depositor
    Ute Hochgeschwender
    Article
    Petersen et al Front Neurol. 2022 Jan 20;12:792643. doi
    Inserts
    Gaussia luciferase, slow burn
    Volvox Channelrhodopsin 1
    Enhanced Yellow Fluorescent Protein
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    114103-plasmid-map-sequence-id-252555

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  48. pGfa2-nLac

    Plasmid
    #53126
    Purpose
    Expression of LacZ driven by the human Gfa2 promoter. LacZ can also be replaced with the gene of interest for targeting transgene expression to astrocytes in mice.
    Depositor
    Michael Brenner
    Article
    Brenner et al J Neurosci. 1994 Mar;14(3 Pt 1):1030-7.
    Insert
    Gfa2 (GFAP Human)
    Use
    Mouse Targeting
    Tags
    LacZ, NLS, and mP1
    Expression
    Mammalian
    Mutation
    contains -2163 to +47 (the human gfa2 segment), w…
    Promoter
    human GFAP
    Available since
    May 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    53126-plasmid-map-sequence-id-82755

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Showing: 11951 - 12000 of 28583 results