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Plasmid#123750PurposeEncodes mScarlet as a Type 3 part to be used in the MTK systemDepositorInsertmScarlet
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC1802_pmax-RBFOX1N-dCasRx-C
Plasmid#118635PurposeTransient transfection; Expresses RBFOX1N-dCasRx-C; CAGGS promoterDepositorInsertRBFOX1N-dCasRx-C
UseCRISPRTagsExpressionMammalianMutationPromoterCAGGSAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pET-15b_LC3B
Plasmid#73949PurposeFor recombinant expression of human LC3B/MAP1LC3B in E. coliDepositorInsertLC3B (MAP1LC3B Human)
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 IGF1R-BirA(R118G)-HA
Plasmid#109232PurposeThis construct can be used in the BioID process to identify proteins that interact with the Insulin-like Growth Factor 1 Receptor (IGF1R).DepositorInsertIGF1R (IGF1R Human)
UseTagsBirA(R118G) (highly promiscuous form) and HAExpressionMammalianMutationPromoterT7Available sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
MOS
Plasmid#64120PurposeA modified episomal (EBNA1/oriP) vector expressing human OCT4 and SOX2 genesDepositorUseTagsHA and MycExpressionMammalianMutationPromoterSFFVAvailable sinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-Flag-SPRTN-wt
Plasmid#110214PurposeExpression in mammalian cells of full-length wild-type SPRTN protein with a Flag-tag at N-terminus.DepositorInsertSPRTN (SPRTN Human)
UseTagsExpressionMammalianMutationP296L (see depositor comments below)PromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
FlucDM-HA-GFP11-N1
Plasmid#91956PurposeExpresses aggregation-prone FlucDM-HA-GFP11 in mammalian cellsDepositorInsertFlucDM-HA-GFP11
UseTagsExpressionMammalianMutationR188Q,R261QPromoterCMVAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEN244 - CTCF-AID[71-114]-eGFP-FRT-Blast-FRT targeting construct
Plasmid#92140PurposeTargeting vector to introduce an AID-eGFP cassette at the mouse CTCF locus using BLASTICIDIN selection. Auxin-inducible degron system.DepositorInsertAID[71-114]-eGFP
UseMouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
Cerulean-Venus-FRET-10
Plasmid#58177PurposeLocalization: Cytosolic FRET, Excitation: , Emission:DepositorTypeEmpty backboneUseTagsCerulean and VenusExpressionMammalianMutationPromoterAvailable sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGD-P1/HC-Pro
Plasmid#196328Purpose35S promoter-driven expression of the tobacco etch virus P1/HC-Pro RNA silencing suppressorDepositorInserttobacco etch virus P1/HC-Pro
UseTagsExpressionPlantMutationPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
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YFVdelNS1-mVenus
Plasmid#179950PurposepCCI vector encoding YFV-17D lacking nonstructural protein 1 (NS1) but with mVenusDepositorInsertYFV-17D lacking Nonstructural protein 1 (NS1) but with mVenus
UseTransneuronal tracingTagsExpressionMutationPromoterSP6Available sinceJan. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-dCasRx-ALKBH5-PURO
Plasmid#175582PurposeExpresses dCasRx-ALKBH5 in mammalian cellDepositorInsertdCasRx-ALKBH5
UseCRISPR and LentiviralTagsFLAG-HAExpressionMammalianMutationRfxCas13d R239A/H244A/R858A/H863APromoterpMSCVAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-DVL1-WT
Plasmid#194586PurposeMammalian Expression of mEGFP-DVL1 WT Fusion ProteinDepositorInsertDVL1 (DVL1 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mem-TurboID
Plasmid#149409PurposeMammalian expression of secretory pathway membrane-localized TurboID, N-term HA tag, C-term V5 tag, in mammalian expression plasmid. Amp/Neo selectionDepositorInsertmem-TurboID
UseTagsHA and V5ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO-p53-shRNA-427
Plasmid#25636DepositorInsertp53-shRNA-427 (TP53 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUASattB-ERK-SPARK
Plasmid#106923PurposeExpress ERK-SPARK, an activity reporter of ERK in DrosophilaDepositorInsertERK-SPARK
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO-PTEN-shRNA-3001
Plasmid#25639DepositorInsertPTEN-shRNA-3001 (PTEN Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
FAB(PX-LD)
Plasmid#214426PurposeDetection of peroxisome-lipid droplet contact siteDepositorInsertFKBP-V5
UseTagsCFAST-Halo and FKBP-V5-RspANExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO (pEHA1339)
Plasmid#209088PurposeBackbone plasmid for Tet Responsive transgene expression, FRT integrationDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-phsyn-FLEX-KASH-EGFP-U6-ShGmeb1
Plasmid#129706PurposeExpress cre-dependent KASH-EGFP and Gmeb1 shRNADepositorInsertKASH-EGFP
UseAAVTagsEGFPExpressionMammalianMutationPromoterU6Available sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo
Plasmid#183411PurposepiggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.DepositorInsertTet-on 3G
UseCRISPR and Synthetic Biology; PiggybacTagsExpressionMammalianMutationPromoterEF1AAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLuc
Plasmid#170575PurposeExpress firefly luciferase. Used in MLV-based SARS-CoV-2 pseudovirus assay.DepositorInsertLuc
UseLuciferase and RetroviralTagsNAExpressionMammalianMutationPromoterCMVAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNL4-3 envFS eGFP Δvif/vpr
Plasmid#101344PurposeDeletion from NL4-3 nt 5582-6199 encompassing Vif and Vpr coding sequenceDepositorInsertNL4-3
UseLentiviralTagsExpressionMutationdelta vif/VprPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS306-URA3-PHIS3-PfV-GS-Sapphire
Plasmid#116932PurposeYeast integrative vector for the exression of the 40nm GEM under the HIS3 promoterDepositorInsertPfv
UseTagsSapphireExpressionYeastMutationPromoterHIS3Available sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
REEP5-mEmerald
Plasmid#186964PurposeExpress REEP5/DP1 with C-terminus mEmeraldDepositorInsertReep5 (Reep5 Mouse)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMV PromoterAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW-eGFP
Plasmid#162823PurposeDoxycycline-inducible overexpression of eGFPDepositorInserteGFP
UseLentiviralTagsExpressionMammalianMutationV163APromoterTet ONAvailable sinceFeb. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUB-GFP
Plasmid#11155PurposeMammalian expression vector for expression of GFP (Ubiquitin C promoter)DepositorUseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLinker-2xTy-HXGPRT-LoxP
Plasmid#86664PurposeCRISPR tagging with 2xTy in Toxoplamsa gondiiDepositorInsertLinker-2xTy-HXGPRT 3'UTR
UseExpression in toxoplasma gondiiTagsExpressionMutationPromoterAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHN1002
Plasmid#178831PurposeBacterial expression for human Saposin ADepositorInsertartificial gene encoding human Saposin A (AR Human)
UseTagsHis8, 3CExpressionBacterialMutationCodon-usage was optimized for bacterial expressio…PromoterT7Available sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-CuO-V5 CASC3 siRes1+2 f.l. WT
Plasmid#158540PurposeFor PiggyBac-mediated integration of V5-tagged siRNA-resistant full-length CASC3DepositorInsertCASC3; BTZ; MLN51 (CASC3 Human)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBHM2641
Plasmid#229525PurposeCarries OsTIR1-t2a-mNG-AID*, used to insert TIR1 expressing construct into the C. neoformans genome.DepositorInsertOsTIR1
UseCRISPRTagst2a-mNeonGreen-AID*ExpressionBacterial and YeastMutationF74GPromoterTEF1Available sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2642
Plasmid#229526PurposeCarries OsTIR1-t2a-mNG-mIAA7_opt, used to insert TIR1 expressing construct into the C. neoformans genome.DepositorInsertOsTIR1
UseTagst2a-mNeonGreen-mIAA7_optExpressionBacterial and YeastMutationF74GPromoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2644
Plasmid#229528PurposeCarries AID* tag, empty sgRNA, and NATR markerDepositorInsertAID* - empty sgRNA - NATR
UseTags2xFLAGExpressionBacterial and YeastMutationPromoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2645
Plasmid#229529PurposeCarries mIAA7_opt tag, empty sgRNA, and NATR markerDepositorInsertmIAA7_opt - empty sgRNA - NATR
UseCRISPRTags2xFLAGExpressionBacterial and YeastMutationPromoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2646
Plasmid#229530PurposeCarries mAID_opt tag, empty sgRNA, and NATR markerDepositorInsertmAID_opt - empty sgRNA - NATR
UseTags2xFLAGExpressionBacterial and YeastMutationPromoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2647
Plasmid#229989PurposeCarries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.DepositorInsertOsTIR1
UseTagst2a-mNeonGreen-mIAA7_optExpressionBacterial and YeastMutationF74GPromoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2648
Plasmid#229990PurposeCarries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.DepositorInsertOsTIR1
UseTagst2a-mNeonGreen-mIAA7_optExpressionBacterial and YeastMutationF74GPromoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2649
Plasmid#229991PurposeCarries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.DepositorInsertOsTIR1
UseTagst2a-mNeonGreen-mIAA7_optExpressionBacterial and YeastMutationF74GPromoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2650
Plasmid#229992PurposeCarries AID* tag, empty sgRNA, and HYGR markerDepositorInsertAID* - empty sgRNA - HYGR
UseTags2xFLAGExpressionBacterial and YeastMutationPromoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2651
Plasmid#229993PurposeCarries AID* tag, empty sgRNA, and NEOR markerDepositorInsertAID* - empty sgRNA - NEOR
UseTags2xFLAGExpressionBacterial and YeastMutationPromoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2652
Plasmid#229994PurposeCarries AID* tag, empty sgRNA, and amdS markerDepositorInsertAID* - empty sgRNA - amdS
UseTags2xFLAGExpressionBacterial and YeastMutationPromoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
AiP14489 - pAAV-AiE0675m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN4489)
Plasmid#230399PurposeAiE0675m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cellsInsertSYFP2
UseAAVTagsExpressionMutationPromoterminBGAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
AiP12927 - pAAV-AiE0078m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2927)
Plasmid#230365PurposeAiE0078m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cellsInsertSYFP2
UseAAVTagsExpressionMutationPromoterminBGAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
AiP12328 - pAAV-3xSP10ins-AiE0354h_3xC2-minRho-SYFP2-WPRE3-BGHpA (Alias: CN2328)
Plasmid#230361PurposeAiE0354h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cellsInsertSYFP2
UseAAVTagsExpressionMutationPromoterminBGAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZa pBAD csgA CBDcex pLac Ara csgGEF
Plasmid#226378PurposePlasmid carries csgA and csgG,E,F genes of curli operonDepositorInsertcsgA and csgG,E,F genes of curli operon
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
AiP14478 - pAAV-AiE0456m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN4478)
Plasmid#229923PurposeAiE0456m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific brain cell typesInsertSYFP2
UseAAVTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
AiP13028 - pAAV-AiE0461m_3xC3-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3028)
Plasmid#224022PurposeAiE0461m_3xC3 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neuronsInsertSYFP2
UseAAVTagsExpressionMutationNAPromoterminBGAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRS-XylS-dCas9
Plasmid#220196Purposemini-Tn7 dCas9 expression vector - XylS/pM promoterDepositorInsertPseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
UseCRISPRTagsExpressionBacterialMutationPseudomonas aeruginosa codon optimizedPromoterXylS/pMAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only