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Addgene

pcDNA3.1 IGF1R-BirA(R118G)-HA
(Plasmid #109232)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 109232 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1 MCS-BirA(R118G)-HA
  • Backbone manufacturer
    Kyle Roux, Addgene plasmid #36047
  • Backbone size w/o insert (bp) 5333
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IGF1R
  • Alt name
    Insulin-like Growth Factor 1 Receptor
  • Species
    H. sapiens (human)
  • Entrez Gene
    IGF1R (a.k.a. CD221, IGFIR, IGFR, JTK13)
  • Promoter T7
  • Tags / Fusion Proteins
    • BirA(R118G) (highly promiscuous form) (C terminal on insert)
    • HA (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1 IGF1R-BirA(R118G)-HA was a gift from Victor Dzau (Addgene plasmid # 109232 ; http://n2t.net/addgene:109232 ; RRID:Addgene_109232)
  • For your References section:

    The proximity-labeling technique BioID identifies sorting nexin 6 as a member of the insulin-like growth factor 1 (IGF1)-IGF1 receptor pathway. Bareja A, Hodgkinson CP, Soderblom E, Waitt G, Dzau VJ. J Biol Chem. 2018 Mar 12. pii: RA118.002406. doi: 10.1074/jbc.RA118.002406. 10.1074/jbc.RA118.002406 PubMed 29530981