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PurposeThis construct can be used in the BioID process to identify proteins that interact with the Insulin-like Growth Factor 1 Receptor (IGF1R).
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 109232 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1 MCS-BirA(R118G)-HA
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Backbone manufacturerKyle Roux, Addgene plasmid #36047
- Backbone size w/o insert (bp) 5333
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIGF1R
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Alt nameInsulin-like Growth Factor 1 Receptor
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SpeciesH. sapiens (human)
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Entrez GeneIGF1R (a.k.a. CD221, IGFIR, IGFR, JTK13)
- Promoter T7
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Tags
/ Fusion Proteins
- BirA(R118G) (highly promiscuous form) (C terminal on insert)
- HA (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1 IGF1R-BirA(R118G)-HA was a gift from Victor Dzau (Addgene plasmid # 109232 ; http://n2t.net/addgene:109232 ; RRID:Addgene_109232) -
For your References section:
The proximity-labeling technique BioID identifies sorting nexin 6 as a member of the insulin-like growth factor 1 (IGF1)-IGF1 receptor pathway. Bareja A, Hodgkinson CP, Soderblom E, Waitt G, Dzau VJ. J Biol Chem. 2018 Mar 12. pii: RA118.002406. doi: 10.1074/jbc.RA118.002406. 10.1074/jbc.RA118.002406 PubMed 29530981