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pBABEpuro-ARAF_S214F

Plasmid

#51078

Purpose

mammalian expression of ARAF S214F

Depositor

Matthew Meyerson

Article

Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

Insert

ARAF (ARAF Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

S214F

Promoter

Available since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pX330-U6-E690_guide-CBh-hSpCas9

Plasmid

#188545

Purpose

Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid E690

Depositor

Darrell Kotton

Article

Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

Insert

gRNA

Use

CRISPR

Tags

T2A-EGFP

Expression

Mutation

Promoter

U6

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-U6-W308_guide-CBh-hSpCas9

Plasmid

#188546

Purpose

Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid W308

Depositor

Darrell Kotton

Article

Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

Insert

gRNA

Use

CRISPR

Tags

T2A-EGFP

Expression

Mutation

Promoter

U6

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-U6-L101_guide-CBh-hSpCas9

Plasmid

#188547

Purpose

Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid L101

Depositor

Darrell Kotton

Article

Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

Insert

gRNA

Use

CRISPR

Tags

T2A-EGFP

Expression

Mutation

Promoter

U6

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-GG-hUBC-EBFP2

Plasmid

#212314

Purpose

Lentiviral backbone with EBFP2 reporter

Depositor

Andrew Wilson

Article

Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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MTOR-F2108L_DARIC(1)_CD19-scFv_AAV6_Donor

Plasmid

#211905

Purpose

Vector for AAV6 donor production. Targeted CD19-scFv DARIC transgene integration to the MTOR locus with the dominant rapamycin resistance MTOR-F2108L mutation via homology-directed repair.

Depositor

Yannick Doyon

Article

Levesque et al bioRxiv 2023.09.14.557485

Insert

DARIC-CD19-scFv

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hPGK1

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-DNAJC5-ΔJ (Δ14-82)

Plasmid

#205719

Purpose

Mammalian expression of DNAJC5-ΔJ (Δ14-82)

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Use

Tags

Expression

Mammalian

Mutation

Δ14-82

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-DNAJC5-L115R

Plasmid

#205708

Purpose

Mammalian expression of DNAJC5 L115R

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Use

Tags

Expression

Mammalian

Mutation

L115R

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-DNAJC5-L116Δ

Plasmid

#205709

Purpose

Mammalian expression of DNAJC5 L116Δ

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Use

Tags

Expression

Mammalian

Mutation

L116Δ

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-KR-Linker-eGFP

Plasmid

#176066

Purpose

EGFP fused to the C-terminus of a KR domain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

KR

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti_Neo_WT_ZFP64_CR

Plasmid

#118694

Purpose

Express sgZFP64e2 resistant cDNA

Depositor

Christopher Vakoc

Article

Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

Insert

ZFP64 (ZFP64 Human)

Use

Lentiviral

Tags

Expression

Mutation

sgZFP64e2 targeting site was synonymous mutated

Promoter

EF1a

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti_Neo_ZFP64_N_3XFLAG

Plasmid

#118700

Purpose

Express 3xflag tagged ZFP64

Depositor

Christopher Vakoc

Article

Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

Insert

ZFP64 (ZFP64 Human)

Use

Lentiviral

Tags

3XFLAG was fused to the N terminal of ZFP64 cDNA

Expression

Mutation

Promoter

EF1A

Available since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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LRG-2.1T-sgTAF12(human)#4.5

Plasmid

#105987

Purpose

lentivirally express gRNA targeting human TAF12 HFD with GFP marker

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

gRNA targeting human TAF12-HFD

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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LRG-neo-sgTAF12(mouse)#4.1

Plasmid

#105588

Purpose

lentivirally express gRNA targeting human TAF12 HFD with GFP marker and neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

gRNA targeting TAF12 HFD

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-puro-N3FLAG-MYB-del(TAD)

Plasmid

#105597

Purpose

retrovirally express mouse MYB-del(TAD) with 3*FLAG tag at N terminal and puro resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

MYB without TAD domain (Myb Mouse)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Mutation

deletion of TAD (AA 275-327)

Promoter

MSCV-LTR

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-PIG-TAF4(827-909)

Plasmid

#105616

Purpose

retrovirally express express mouse TAF4 HFD with GFP marker and puro resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF4 HFD-827-909 (Taf4 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

MSCV-LTR

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-ARAF_D429A

Plasmid

#51080

Purpose

mammalian expression of ARAF D429A

Depositor

Matthew Meyerson

Article

Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

Insert

ARAF (ARAF Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

D429A

Promoter

Available since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX-BAIAP2 wt

Plasmid

#31675

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

BAI1-associated protein 2 (BAIAP2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pDY1173_ADAR1p150

Plasmid

#193192

Purpose

Express full length ADAR1p150

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

Insert

ADAR1p150 (ADAR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-SFFV-KRAB-dCas9-P2A-mCherry

Plasmid

#60954

Purpose

2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9 fusion protein and mCherry

Depositor

Jonathan Weissman

Article

Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

Insert

KRAB-dCas9-P2A-mCherry fusion

Use

CRISPR and Lentiviral

Tags

2x NLS, HA, KRAB domain, and mCherry

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Jan. 5, 2015

Availability

Academic Institutions and Nonprofits only

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