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LentiCRISPRv2 EGFP sgRNA2

Plasmid

#164933

Purpose

Expresses EGFP sgRNA2 in mammalian cells

Depositor

Ji Luo

Article

Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

Insert

sgRNA2 targeting Enhanced green fluorescent protein (verified for knockout)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EFS promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgLKB1 clone1

Plasmid

#162125

Purpose

Lentiviral sgRNA plasmid targeting human LKB1

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgLKB1 (STK11 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2 RB1 sgRNA #1

Plasmid

#202516

Purpose

Expressing Cas9 and sgRNA targeting human RB1 gene

Depositor

Ming Chen

Article

Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

Insert

RB1 sgRNA (RB1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-Stuffer-HepmCherry

Plasmid

#192825

Purpose

Contains stuffer sequence into which sgRNAs can be cloned and expresses mCherry from hepatocyte-specific promoter

Depositor

Kristin Knouse

Article

Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

Insert

mCherry

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Hepatocyte-specific promoter (HS-CRM8-TTRmin modu…

Available since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pAf-CRISPR-phoA

Plasmid

#207991

Purpose

CRISPR vector used with pAf-CRISPR-ctfR1 (#207992) to delete both aflatoxin and cyclopiazonic acid gene clusters of Aspergillus flavus

Depositor

Perng-Kuang Chang

Article

Chang et al Fungal Genet Biol. 2023 Dec 27;170:10386

Insert

phoA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Aspergillus flavus U6

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pSL0426 (CRISPR with spc3)

Plasmid

#130642

Purpose

Expresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA with spacer 3 from the native CRISPR.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

CRISPR(spacer-3)

Use

CRISPR; Transposon

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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p8103 LentiCRISPR v2 sgNT-2

Plasmid

#163313

Purpose

Expresses Cas9 and a non-targeting control guide RNA

Depositor

Elizabeth White

Article

Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

Insert

sgNT-2

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2-Blast-mU6

Plasmid

#206806

Purpose

pLentiCRISPRv2 variant with mU6 promoter driving the sgRNA, and a Blasticidin resistance cassette

Depositor

Jolanda Van Leeuwen

Article

Unlu et al Genome Med. 2023 Oct 12;15(1):78. doi: 1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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POLR2A-N CRISPR pX330

Plasmid

#124495

Purpose

A CRISPR plasmid for targeting the N-terminus coding region of human POLR2A

Depositor

Masato Kanemaki

Article

Nagashima et al J Cell Biol. 2019 Mar 1. pii: jcb.201811

Insert

POLR2A targeting CRISPR

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6 promoter

Available since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti CRISPR sgMTHFD2_1

Plasmid

#106313

Purpose

Express Cas9 and sgRNA targeting MTHFD2

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting MTHFD2 (MTHFD2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-sgLmnb2-HepmCherry

Plasmid

#192830

Purpose

Expresses sgRNA targeting Lmnb2 and mCherry from hepatocyte-specific promoter

Depositor

Kristin Knouse

Article

Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

Insert

mCherry

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 for sgRNA and hepatocyte-specific promoter (HS…

Available since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-sgMaob-HepmCherry

Plasmid

#192829

Purpose

Expresses sgRNA targeting Maob and mCherry from hepatocyte-specific promoter

Depositor

Kristin Knouse

Article

Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

Insert

mCherry

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 for sgRNA and hepatocyte-specific promoter (HS…

Available since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR - EGFP sgRNA 3

Plasmid

#51762

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

Depositor

Feng Zhang

Article

Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

Inserts

Cas9
Puromycin resistance
EGFP sgRNA 3

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EFS and U6

Available since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

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Lenti-CRISPR-v2-gYap-1

Plasmid

#166481

Purpose

Expresses spCas9 and a gRNA targeting mouse Yap

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

gRNA for mouse Yap (Yap1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-Stuffer-HepmTurquoise2

Plasmid

#192826

Purpose

Contains stuffer sequence into which sgRNAs can be cloned and expresses mTurquoise2 from hepatocyte-specific promoter

Depositor

Kristin Knouse

Article

Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

Insert

mTurquoise2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Hepatocyte-specific promoter (HS-CRM8-TTRmin modu…

Available since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-CRISPR-V2-gYap-2

Plasmid

#166482

Purpose

Expresses spCas9 and a gRNA targeting mouse Yap

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

gRNA for mouse Yap (Yap1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pZR064_CROPseq-sgCD4-CRISPRi

Plasmid

#180270

Purpose

Lentiviral CROPseq vector expressing CD4 targeting sgRNA for CRISPRi

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

CD4 sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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lenticrispr-wt-ltr-puro

Plasmid

#173428

Purpose

Expresses Cas9 and guide RNA, contains intact LTR

Depositor

Jakob Reiser

Article

Iaffaldano et al Front Genome Ed. 2023 Jul 13;5:1218328.

Insert

S. pyogenes sgRNA cassette

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR - EGFP sgRNA 6

Plasmid

#51765

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

Depositor

Feng Zhang

Article

Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

Inserts

Cas9
Puromycin resistance
EGFP sgRNA 6

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EFS and U6

Available since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR-v2-Blast-Puro

Plasmid

#167186

Purpose

Lentiviral expression of sgRNA with blasticidin and puromycin resistance genes

Depositor

Yin Shen

Article

Ren et al Sci Adv. 2021 Sep 17;7(38):eabi4360. doi

Insert

Blasticidin S deaminase

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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