Addgene

Showing: 461 - 480 of 540 results

Results per page:

Sort results by:

pMC051-HisMS2_PLP_pac_LC

Plasmid

#128234

Purpose

Lower Copy expression construct for MS2 phage like particles with Esp3I cloning sites for packaged RNA.

Depositor

Paul Freemont

Article

de Martin Garrido et al Mol Microbiol. 2019 Oct 16. doi: 10.1111

Inserts

Maturation Protein
Coat Protein Dimer

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
plIVMD156

Plasmid

#160934

Purpose

In vivo mRNA display Gateway Destination construct

Depositor

Saeed Tavazoie

Article

Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

Insert

MCP-Gateway cloning site-HIS tag, 3'UTR MS2 Stem Loop

Use

Tags

HIS tag, 3'UTR MS2 Stem Loop

Expression

Yeast

Mutation

Promoter

MET25

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB1K3_pBen-sfGFP_J23101-BenR

Plasmid

#128125

Purpose

Expressing sfGFP under control of benzoate responsive promoter (pBen) and expressing BenR transcription factor gene under constitutive promoter J23101, and RBS B0032

Depositor

Jean-Loup Faulon

Article

Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

Insert

sfGFP and BenR

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pBen and J23101

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
plIVMD164

Plasmid

#160936

Purpose

In vivo mRNA display construct for GFP

Depositor

Saeed Tavazoie

Article

Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

Insert

MCP-sfGFP-HIS tag, 3'UTR MS2 Stem Loop

Use

Tags

HIS tag, 3'UTR MS2 Stem Loop

Expression

Yeast

Mutation

Promoter

MET25

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB4C5_J23101-CocE

Plasmid

#128129

Purpose

Expressing CocE enzyme (cocaine to benzoate) gene under control of the constitutive promoter J23101, and RBS B0032

Depositor

Jean-Loup Faulon

Article

Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

Insert

CocE

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23101

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MCP-YPet

Plasmid

#215747

Purpose

Expresses MS2 coat protein (MCP) fused to YPet

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

YPet

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
CTAG_eGFP-HDEL

Plasmid

#136097

Purpose

FP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_eGFP(noATG,noStop)-HDEL

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CTAG_mTurquoise2-HDEL

Plasmid

#136105

Purpose

FP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_mTurquoise2(noATG,noStop)-HDEL

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
CTAG_mVenus-HDEL

Plasmid

#136107

Purpose

FP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_mVenus(noATG,noStop)-HDEL

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
plIVMD163

Plasmid

#160935

Purpose

In vivo mRNA display construct for mCherry

Depositor

Saeed Tavazoie

Article

Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

Insert

MCP-mCherry-HIS tag, 3'UTR MS2 Stem Loop

Use

Tags

HIS tag, 3'UTR MS2 Stem Loop

Expression

Yeast

Mutation

Promoter

MET25

Available since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB4C5_J23101-vdh

Plasmid

#128130

Purpose

Expressing vdh gene (for the enzyme transforming benzaldehyde to benzoate) under control of the constitutive promoter J23101, and RBS B0032

Depositor

Jean-Loup Faulon

Article

Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

Insert

vdh

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23101

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEAST_J23101-HipO

Plasmid

#128133

Purpose

The cell-free adapted backbone, pBEAST, expressing HipO (the enzyme converting hippurate to benzoate) under control of the constitutive promoter J23101, and RBS B0032

Depositor

Jean-Loup Faulon

Article

Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

Insert

HipO

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23101

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
JB55

Plasmid

#128929

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

RhoATN-BirA*-HA-MAO (RHOA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
phage ubc nls ha 2xmcp HALO

Plasmid

#64540

Purpose

Used to label reporter mRNAs. Lentiviral expression of the MCP protein fused to HALO.

Depositor

Jeffrey Chao

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

MCP

Use

Lentiviral

Tags

HA, HALO, and NLS

Expression

Mammalian

Mutation

Promoter

human ubiquitin C promoter

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
phage UBC NLS-HA-2XMCP-tagRFPt

Plasmid

#64541

Purpose

Used to label reporter mRNAs. Lentiviral expression of the MCP protein fused to tagRFP.

Depositor

Jeffrey Chao

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

MCP

Use

Lentiviral

Tags

HA, NLS, and tagRFP-T

Expression

Mammalian

Mutation

Promoter

human ubiquitin C promoter

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMALcPP-MS2cp

Plasmid

#203351

Purpose

Expresses His/MBP-tagged MS2-CP V75E/A81G variant in bacteria

Depositor

Thomas Cech

Article

Hemphill et al Proc Natl Acad Sci U S A. 2023 Jun 27;12

Insert

MS2 coat protein

Use

Tags

Hx6-MBP

Expression

Bacterial

Mutation

V75E and A81G, to prevent capsid assembly

Promoter

Available since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
MCP-mCherry-TIAL1

Plasmid

#207669

Purpose

To express fluorescently labeled MS2 coat binding protein (MCP) fused with RNA binding protein TIAL1

Depositor

Christine Mayr

Article

Horste et al Mol Cell. 2023 Dec 21;83(24):4509-4523.e

Insert

TIAL1 (TIAL1 Human)

Use

Tags

MCP-mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
NLS-HA-tdMCP-tdTomato

Plasmid

#183938

Purpose

tandem MS2 binding protein labeled with tdTomato, containing a NLS and a HA-tag

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

tdMCP-tdTomato (cp Synthetic)

Use

Tags

NLS-HA

Expression

Mammalian

Mutation

none

Promoter

CMV (TATA box removed)

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJRTCLCrVA.008

Plasmid

#22841

Depositor

Niki Robertson

Article

Tuttle et al Plant Physiol. 2008 Sep . 148(1):41-50.

Insert

A DNA (with MCS)

Use

Cotton vigs vector

Tags

Expression

Bacterial

Mutation

Coat Protein (AR1 gene) of Cotton Leaf Crumple Vi…

Promoter

Available since

June 14, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pRW006-SM-destination-pro35S-Cas9

Plasmid

#104439

Purpose

Destination vector expressing plant-codon-optimized Cas9 under 35S promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free

Depositor

Detlef Weigel

Article

Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial and Plant

Mutation

Promoter

35S

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge