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pαH-S-GSAS-D614G-I692V

Plasmid

#171746

Purpose

Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-I692V variant

Depositor

Priyamvada Acharya

Article

Gobeil et al Science. 2021 Jun 24. pii: science.abi62

Insert

Spike (S-GSAS-D614G-I692V variant) (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

Expression

Mammalian

Mutation

Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

Promoter

Available since

July 15, 2021

Availability

Academic Institutions and Nonprofits only

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pαH-S-GSAS-D614G-Y453F

Plasmid

#171745

Purpose

Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-Y453F variant

Depositor

Priyamvada Acharya

Article

Gobeil et al Science. 2021 Jun 24. pii: science.abi62

Insert

Spike (S-GSAS-D614G-Y453F variant) (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

Expression

Mammalian

Mutation

Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

Promoter

Available since

July 15, 2021

Availability

Academic Institutions and Nonprofits only

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CILK1-R272Q

Plasmid

#214911

Purpose

expression of the mutant variant of CILK1

Depositor

Pavel Krejčí

Article

Paige Taylor et al Hum Mol Genet. 2016 Sep 15;25(18):3998-4

Insert

CILK1 (CILK1 Human)

Use

Tags

Myc-FLAG

Expression

Mammalian

Mutation

R272Q substitution

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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CILK1-E80K

Plasmid

#214910

Purpose

expression of the mutant variant of CILK1

Depositor

Pavel Krejčí

Article

Paige Taylor et al Hum Mol Genet. 2016 Sep 15;25(18):3998-4

Insert

CILK1 (CILK1 Human)

Use

Tags

Myc-FLAG

Expression

Mammalian

Mutation

E80K substitution

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-sgBrip1#2/Cre

Plasmid

#193204

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Brip1 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgBrip1#2 (Brip1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgFam135b#2/Cre

Plasmid

#193214

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Fam135b gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgFam135b#1 (Fam135b Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgErich3#1/Cre

Plasmid

#193211

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Erich3 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgErich3#1 (4922501L14Rik Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgFlt4#2/Cre

Plasmid

#193216

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Flt4 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgFlt4#2 (Flt4 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgErich3#2/Cre

Plasmid

#193212

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Erich3 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgErich3#2 (4922501L14Rik Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgCol11a1#1/Cre

Plasmid

#193205

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Col11a1 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgCol11a1#1 (Col11a1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgCol11a1#2/Cre

Plasmid

#193206

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Col11a1 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgCol11a1#2 (Col11a1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgCrebbp#1/Cre

Plasmid

#193209

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Crebbp gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgCrebbp#1 (Crebbp Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgApc#2/Cre

Plasmid

#193202

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Apc gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgApc#2 (Apc Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgBrip1#1/Cre

Plasmid

#193203

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Brip1 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgBrip1#1 (Brip1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgFlt4#1/Cre

Plasmid

#193215

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Flt4 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgFlt4#1 (Flt4 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgGrin2a#1/Cre

Plasmid

#193217

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Grin2a gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgGrin2a#1 (Grin2a Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgFam135b#1/Cre

Plasmid

#193213

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Fam135b gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgFam135b#1 (Fam135b Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pαH-S-GSAS-B.1.351.v4

Plasmid

#173788

Purpose

Mammalian expression of SARS-CoV-2 Spike protein South African variant version 4

Depositor

Priyamvada Acharya

Article

Gobeil et al Science. 2021 Jun 24. pii: science.abi62

Insert

Spike (S-GSAS-B.1.351 variant version 4) (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

Expression

Mammalian

Mutation

Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

Promoter

Available since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSG360zeo

Plasmid

#27154

Depositor

Michael Glickman

Article

Barkan et al J Bacteriol. 2010 Jul . 192(14):3661-8.

Type

Empty backbone

Use

Mycobacterial ko vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSG360

Plasmid

#27155

Depositor

Michael Glickman

Article

Barkan et al J Bacteriol. 2010 Jul . 192(14):3661-8.

Type

Empty backbone

Use

Mycobacterial ko vector

Tags

Expression

Mutation

Promoter

Available since

March 3, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge