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Plasmid#86357PurposeEncodes gRNA for 3' target of human SOX5DepositorInsertgRNA against SOX5 (SOX5 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHL1632
Plasmid#62829PurposeLim Lab plasmid: AmpR + PLlacO-1::RBS(st7) gfp::Asp terminator + ColE1DepositorInsertgfp
UseSynthetic BiologyTagsst7 RBSExpressionBacterialMutationPromoterpLlacO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
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SRP19 in pLX311
Plasmid#233292PurposeExpression of WT SRP19DepositorInsertSRP19 (SRP19 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterE1FaAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
K560-Halo-Strep
Plasmid#219681PurposeMammalian expression of KIF5B(1-560)-HaloTag-StrepDepositorInsertKIF5B (KIF5B Human)
UseTagsHaloTag, Strep tagExpressionMammalianMutation1-560PromoterCMV & T7Available sinceDec. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CLIPf-KIF5A(1-573)
Plasmid#219683PurposeMammalian expression of CLIPf-KIF5A(1-573)DepositorInsertKIF5A (Kif5a Mouse)
UseTagsCLIPfExpressionMammalianMutation1-573PromoterCMV & T7Available sinceDec. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CLIPf-KIF5A
Plasmid#200790PurposeMammalian expression of CLIPf-KIF5ADepositorInsertKIF5A (Kif5a Mouse)
UseTagsCLIPfExpressionMammalianMutationPromoterCMV & T7Available sinceDec. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSET_SynPspA
Plasmid#223812PurposeBacterial expression of SynPspA with N-terminal 10xHis-tag and EK cleavage siteDepositorInsertSynPspA
UseTags10xHis and NusAExpressionBacterialMutationPromoterAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJET-TtrpC
Plasmid#213467PurposeVector containing the TrpC terminator for Golden Gate cloning in the Fg vectorDepositorInsertTerminator of tryptophan synthase of Aspergillus nidulans
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJET-PgpdA
Plasmid#213466PurposeVector containing the gpdA promoter for Golden Gate cloning in the Fg vectorDepositorInsertPromoter of the glyceraldehyde-3-phosphate dehydrogenase
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJET-PtrpC
Plasmid#213465PurposeVector containing the TrpC promoter for Golden Gate cloning in the Fg vectorDepositorInsertPromoter of tryptophan synthase of Aspergillus nidulans
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-DDX5(PrD)
Plasmid#166167PurposeGateway entry vector for N. furzeri DDX5-PrD(535-634) mutantDepositorArticleInsertDDX5-PrD(535-634)
UseGateway entry vectorTagsExpressionMutationPrD only 1606-1902PromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-DDX5ΔPrD
Plasmid#166164PurposeGateway entry vector for N. furzeri DDX5ΔPrD(1-535) mutantDepositorArticleInsertDDX5ΔPrD(1-535)
UseGateway entry vectorTagsExpressionMutationdeletion of 1606-1902PromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-DDX5ΔIDR
Plasmid#166165PurposeGateway entry vector for N. furzeri DDX5ΔIDR(1-483) mutantDepositorArticleInsertDDX5ΔIDR(1-483)
UseGateway entry vectorTagsExpressionMutationdeletion of 1450-1902PromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-DDX5(IDR)
Plasmid#166166PurposeGateway entry vector for N. furzeri DDX5-IDR(483-634) mutantDepositorArticleInsertDDX5-IDR(483-634)
UseGateway entry vectorTagsExpressionMutationIDR only 1450-1902PromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
BcC _pET29b
Plasmid#215403PurposeExpression construct for BcC gene, codon optimized for expression in E. coliDepositorInsertBcC
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pITESceIcmd
Plasmid#218288PurposeIntroduction of a cyanamide-inducible I-SceI expression cassette, use in combination with pITEdv1DepositorInsertHO(-253, -1)-tSynth3UseTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
UMG-SP-3_pET29b
Plasmid#215435PurposeExpression construct for UMG-SP-3 gene, codon optimized for expression in E. coliDepositorInsertUMG-SP-3
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
OoH_pET29b
Plasmid#215424PurposeExpression construct for OoH gene, codon optimized for expression in E. coliDepositorInsertOoH
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Ph-NylC_pET28a
Plasmid#215425PurposeExpression construct for Ph-NylC gene, codon optimized for expression in E. coliDepositorInsertPh-NylC
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
ReU_pET29b
Plasmid#215426PurposeExpression construct for ReU gene, codon optimized for expression in E. coliDepositorInsertReU
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
ThcCut1_pET29b
Plasmid#215429PurposeExpression construct for ThcCut1 gene, codon optimized for expression in E. coliDepositorInsertThcCut1
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
ThcCut2 _pET29b
Plasmid#215430PurposeExpression construct for ThcCut2 gene, codon optimized for expression in E. coliDepositorInsertThcCut2
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
TtA_pET29b
Plasmid#215432PurposeExpression construct for TtA gene, codon optimized for expression in E. coliDepositorInsertTtA
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
MsmegA _pET29b
Plasmid#215411PurposeExpression construct for MsmegA gene, codon optimized for expression in E. coliDepositorInsertMsmegA
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Nc-NylC_pET28a
Plasmid#215412PurposeExpression construct for Nc-NylC gene, codon optimized for expression in E. coliDepositorInsertNc-NylC
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
NfPolyA_pET21b
Plasmid#215413PurposeExpression construct for NfPolyA gene, codon optimized for expression in E. coliDepositorInsertNfPolyA
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
NylB'_pET21b
Plasmid#215416PurposeExpression construct for NylB' gene, codon optimized for expression in E. coliDepositorInsertNylB'
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AfNitB_pET29b
Plasmid#215401PurposeExpression construct for AfNitB gene, codon optimized for expression in E. coliDepositorInsertAfNitB
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AoC_pET29b
Plasmid#215402PurposeExpression construct for AoC gene, codon optimized for expression in E. coliDepositorInsertAoC
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
M-NylC_pET28a
Plasmid#215409PurposeExpression construct for M-NylC gene, codon optimized for expression in E. coliDepositorInsertM-NylC
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
MCP-YPet
Plasmid#215747PurposeExpresses MS2 coat protein (MCP) fused to YPetDepositorInsertYPet
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO FLAG-RPS28
Plasmid#203156PurposeN-FLAG-RPS28 in inducible pcDNA5 vector for Flp-In integrationDepositorInsertRPS28 (RPS28 Human)
UseTags1X FLAG tag with GGGGS linkerExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRBad-C-DiB-RM
Plasmid#168478PurposeC-fragment of the DiB-RM‐split protein. This plasmid needs to be co-transformed with Plasmid 168476: pET11a-N-DiB-RM to obtain DiB-RM‐split proteinDepositorInsertC-fragment of DiB-RM
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET11a-N-DiB-RM
Plasmid#168476PurposeN-fragment of the DiB-RM‐split protein. This plasmid needs to be co-transformed with Plasmid 168478: pMRBad-C-DiB-RM to obtain DiB-RM‐split proteinDepositorInsertN-fragment of DiB-RM
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-rsAKARev(T/A)
Plasmid#166232PurposeExpresses reversibly switchable A-kinase activity reporter with EV-linker inactive T/A mutant (rsAKARev(T/A)) in mammalian cellsDepositorInsertReversibly switchable A-kinase activity reporter with EV-linker inactive T/A mutant
UseTagsExpressionMammalianMutationchanged Threonine 506 in rsAKARev to AlaninePromoterCMVAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEarleyGate100-pSpoA-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
Plasmid#140170PurposeExpresses a regulatable secretory cargo for Arabidopsis thalianaDepositorInsertpSpoA-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEarleyGate100-pSpoA-DsRed-Express2-FKBP(LV)(C22V)
Plasmid#140169PurposeExpresses a regulatable secretory cargo (without an ER export signal) for Arabidopsis thalianaDepositorInsertpSpoA-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-pOst1-ESCargo(Orange)
Plasmid#140157PurposeExpresses a regulatable secretory cargo for yeast at the TRP1 locusDepositorInsertpOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac128-pOst1-ESCargo(Orange)
Plasmid#140158PurposeExpresses a regulatable secretory cargo for yeast at the LEU2 locusDepositorInsertpOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only