Addgene

Showing: 461 - 480 of 11868 results

Results per page:

Sort results by:

Anti-QKI-7 [N183/15R-2b]

Plasmid

#219485

Purpose

Mammalian Expression Plasmid of anti-QKI-7 (Human) subclass-switched IgG2b R-mAb. Derived from hybridoma N183/15.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-QKI-7 (Homo sapiens) recombinant mouse monoclonal antibody. (Qki Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-GFP [N86/34R]

Plasmid

#220419

Purpose

Mammalian Expression Plasmid of anti-GFP (Jellyfish) IgG2a R-mAb. Derived from hybridoma N86/34

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody.

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-EGFP-NEMO-D304N

Plasmid

#187898

Purpose

Mammalian cell expression of D304N mutation in NEMO

Depositor

Erika Holzbaur

Article

Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

Insert

HA-EGFP-NEMO-D304N (Ikbkg Mouse)

Use

Tags

EGFP and HA

Expression

Mammalian

Mutation

D304N

Promoter

CMV

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-3a_mb2m

Plasmid

#211368

Purpose

Expresses murine b2m

Depositor

Adnane Achour

Insert

b2m (B2m Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pB80-KIF1A(1-365)-VVD52C-mCherry-SSPB(micro)

Plasmid

#174633

Purpose

Poorly reversible opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

KIF1A(1-365)-VVD52C-mCherry-SSPB(micro) (Kif1a Synthetic, Mouse)

Use

Tags

mCherry-SSPB(micro)

Expression

Mammalian

Mutation

mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…

Promoter

Chicken beta-actin

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-mROCK2

Plasmid

#101296

Purpose

Mammalian expression of Rock2-GFP

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock2 (Rock2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-MAP1B

Plasmid

#44396

Depositor

Phillip Gordon-Weeks

Article

Scales et al J Cell Sci. 2009 Jul 15;122(Pt 14):2424-

Insert

MAP1B (Map1b Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 21, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Egr1

Plasmid

#11729

Depositor

Eileen Adamson

Article

Yu et al Mol Cell. 2004 Jul 2. 15(1):83-94.

Insert

Egr-1 (Egr1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-SYN-eGFP-Kindlin 1

Plasmid

#203733

Purpose

AAV vector plasmid expressing mouse kindlin-1 fused to eGFP under the human synapsin (SYN) promoter

Depositor

Bart Nieuwenhuis, Richard Eva

Insert

Fermt1 (Fermt1 Mouse)

Use

AAV

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Human synapsin promoter

Available since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pJFRC5-5XUAS-IVS-mCD8::GFP

Plasmid

#26218

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Aug 9. ():.

Insert

mCD8::GFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 19, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-EF1a-BsdCas9-W

Plasmid

#67978

Purpose

Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the N-terminus

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

EF1a-Cas9 cassette, WPRE

Use

CRISPR and Lentiviral

Tags

Cas9 fused with Flag-tag and a NLS at the N termi…

Expression

Mutation

Promoter

EF1a

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-Talin1

Plasmid

#26724

Depositor

Anna Huttenlocher

Article

Franco et al Nat Cell Biol. 2004 Oct . 6(10):977-83.

Insert

Talin (Tln1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pCR-Flag-IKKbeta

Plasmid

#15465

Depositor

Hiroyasu Nakano

Article

Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

Insert

IkB kinase beta (Ikbkb Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 10, 2007

Availability

Academic Institutions and Nonprofits only
pRK172-α-syn-TEV-GFP

Plasmid

#166671

Purpose

Bacterial expression of alpha synuclein coupled to GFP via a TEV site for uptake studies

Depositor

Iris Lindberg

Article

Jarvela et al PLoS One. 2021 Jan 26;16(1):e0241161. do

Insert

mouse synuclein (from V. Lee) (Snca Mouse)

Use

Tags

GFP

Expression

Bacterial

Mutation

added a TEV cleavage site sequence between synucl…

Promoter

CMV

Available since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSIN-TRE-SspB-mCh-p60

Plasmid

#190170

Purpose

Doxycycline-inducible lentivirus vector to express SspBmicro-mCh-p60, a recruitable katanin for microtubule severing, in mammalian cells.

Depositor

Anna Akhmanova

Article

Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

Insert

SspBmicro-mCherry-p60

Use

Lentiviral

Tags

SspBmicro-mCherry

Expression

Mutation

Promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-FLEX-PKIalpha-IRES-nls-mRuby2

Plasmid

#63059

Purpose

Expresses PKIalpha and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone

Depositor

Bernardo Sabatini

Article

Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

Insert

PKIalpha (Pkia Synthetic, Mouse)

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-Puro-mGSDME

Plasmid

#154877

Purpose

Expresses mGSDME in mammalian cells

Depositor

Judy Lieberman

Article

Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

Insert

GSDME (Gsdme Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b-His-3C-irisin

Plasmid

#122612

Purpose

Expresses in E. coli: human irisin with Nt histag and 3C protease site for cleavage

Depositor

Harold Erickson

Article

Albrecht et al Sci Rep. 2015 Mar 9;5:8889. doi: 10.1038

Insert

irisin (ectodomain of FNDC5) (Fndc5 Bovine, Mouse, Human, Rat)

Use

Tags

Nt: histag-3C protease site

Expression

Bacterial

Mutation

Promoter

T7 polymerase

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-Klf4

Plasmid

#13370

Purpose

Retroviral expression of mouse Klf4

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

Kruppel-like factor 4 (gut) (Klf4 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pIgK-2.1-FLAG

Plasmid

#225829

Purpose

Mouse IgK chain specific for chicken gamma globulin (clone 2.1). Used to produce FLAG-tagged monoclonal antibodies for use as tag-specific ELISA standards.

Depositor

Gabriel Victora

Article

Schiepers et al Nature. 2023 Mar;615(7952):482-489. doi:

Insert

Mouse anti-CGG IgK chain, Flag-tagged

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge