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pGWB554

Plasmid

#74884

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with mRFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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TrkB mEGFP

Plasmid

#83952

Purpose

FRET donor for dynamically measuring TrkB activity in neurons

Depositor

Ryohei Yasuda

Article

Harward et al Nature. 2016 Sep 28;538(7623):99-103. do

Insert

Tropomyosin related kinase B (Ntrk2 Mouse)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pSG5 Large T

Plasmid

#9053

Depositor

William Hahn

Insert

large T antigen (SV40gp6 simian virus)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pKC-4.04

Plasmid

#112085

Purpose

Control for NMD sensor: pCMV-3XFLAG-Fluc-betaglobin

Depositor

James Inglese

Article

Baird et al Elife. 2018 Mar 12;7. pii: 33178. doi: 1

Insert

3XFLAG firefly luciferase-beta-globin (HBB Mouse, Human, Photinus pyralis)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pLL7.0: Venus-iLID-CAAX (from KRas4B)

Plasmid

#60411

Purpose

Mammalian Expression of Venus-iLID-CAAX

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

Venus-iLID-CAAX

Use

Cre/Lox and Lentiviral

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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BDNF-SEP

Plasmid

#83955

Purpose

BDNF with pH sensitive fluorophore for visualization of release

Depositor

Ryohei Yasuda

Article

Harward et al Nature. 2016 Sep 28;538(7623):99-103. do

Insert

Brain derived neurotrophic factor (Bdnf Mouse)

Use

Tags

SEP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pnos-Cas9-nos

Plasmid

#62208

Purpose

Expresses Cas9 under control of nanos promoter and 3'UTR. For germ line restricted genome engineering in Drosophila melanogaster.

Depositor

Simon Bullock

Article

Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

Insert

cas9

Use

Tags

Expression

Insect

Mutation

Promoter

nanos

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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HA-Ubiquitin

Plasmid

#18712

Purpose

Mammalian expression of human ubiquitin with HA tag

Depositor

Edward Yeh

Article

Kamitani et al J Biol Chem. 1997 Nov 7. 272(45):28557-6

Insert

ubiquitin (UBB Human)

Use

Tags

HA

Expression

Mammalian

Mutation

The HA-Ubiquitin insert is from the UBB gene. The…

Promoter

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

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pMKO.1 puro GFP shRNA

Plasmid

#10675

Depositor

William Hahn

Article

Masutomi et al Proc Natl Acad Sci U S A. 2005 Jun 7. 10

Insert

GFP shRNA

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK7B2Jnk1a1

Plasmid

#19726

Depositor

Roger Davis

Article

Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

Insert

MKK7B2Jnk1a1 (Map2k7, MAPK8 Mouse, Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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pDEST/hCx43-EGFP-N1

Plasmid

#40907

Depositor

Robin Shaw

Article

Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

Insert

Connexin 43 (GJA1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

No stop codon to create c-terminal fusion

Promoter

CMV

Available since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only

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CMV-HA-hTAF1

Plasmid

#17997

Depositor

Robert Tjian

Article

Ruppert et al Nature. 1993 Mar 11. 362(6416):175-9.

Insert

hTAF1 (TAF1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

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pGL3b-8XGliBS:EGFP

Plasmid

#84602

Purpose

Gli protein-dependent EGFP reporter for analysis of Hedgehog signaling

Depositor

James Chen

Article

Hyman et al Proc Natl Acad Sci U S A. 2009 Aug 18;10

Insert

eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

8X Gli-binding site

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLL7.0: tgRFPt-SSPB WT

Plasmid

#60415

Purpose

Mammalian Expression of tgRFPt-Nano

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

tgRFPt-SSPB

Use

Cre/Lox and Lentiviral

Tags

TagRFPt

Expression

Mammalian

Mutation

Y11K and A15E

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-Nrf2

Plasmid

#67636

Purpose

AAV vector expressing Nrf2 gene

Depositor

Connie Cepko

Article

Xiong et al J Clin Invest. 2015 Apr;125(4):1433-45.

Insert

Nrf2 (Nfe2l2 Mouse)

Use

AAV

Tags

Expression

Mutation

Promoter

CMV

Available since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pLEX307-DPP4-puro

Plasmid

#158451

Purpose

Constitutive lentiviral expression of human DPP4.

Depositor

Sho Iketani, Alejandro Chavez

Insert

DPP4 (DPP4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.V1

Plasmid

#127847

Purpose

non-standard AAV2 rep-AAV-PHP.V1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

Insert

Synthetic construct isolate AAV-PHP.V1 VP1 gene

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

p41

Available since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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T7-p53DD-pcDNA3

Plasmid

#25989

Depositor

William Kaelin

Article

Irwin et al Nature. 2000 Oct 5. 407(6804):645-8.

Insert

tumor protein p53 (TP53 Human)

Use

Tags

T7

Expression

Mammalian

Mutation

truncated cDNA encodes truncated protein (aa 300-…

Promoter

Available since

Oct. 21, 2010

Availability

Academic Institutions and Nonprofits only

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pLenti-puro

Plasmid

#39481

Purpose

Tetracycline inducible 3rd generation lentiviral vector

Depositor

Ie-Ming Shih

Article

Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

V5 and 6XHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pIS0

Plasmid

#12178

Purpose

3'UTR segments of target genes can be inserted into this firefly luciferase reporter to test for their effects on protein production

Depositor

David Bartel

Article

Yekta et al Science. 2004 Apr 23. 304(5670):594-6.

Type

Empty backbone

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only

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