-
Plasmid#161945PurposeContains Type IIS restriction site free elements for IBM staging vectors. Staging plasmid for IBM on mCherryDepositorInsertpSB1C3(BsmBI-)-BsaI-mCherry*(27-218)-BsaI
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCV PIG IMP-1-long-mut
Plasmid#21661DepositorInsertIGF-II mRNA-binding protein 1 (IGF2BP1 Human)
UseRetroviralTagsGFP and IRESExpressionMammalianMutationmutated let-7 binding sites.PromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAimR
Plasmid#98687PurposeExpresses AimR gene from the B. subtilis phage phi3T, with its native promoter, C' His-tagDepositorInsertAimR
UseTagsHis-tagExpressionBacterialMutationPromoterNativeAvailable sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-ChRger3-TS-EYFP
Plasmid#127241PurposeHigh photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.DepositorInsertChRger3-TS-EYFP
UseAAVTagsEYFP and SpyTagExpressionMammalianMutationPromoterCamKIIaAvailable sinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBCV
Plasmid#62659PurposeMammalian expression of FLPo recombinase from the broadly active CAG promoter/enhancerDepositorInsertFLPo
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJL2
Plasmid#184130PurposeFor rescuing null mrp-1 mutation and labelling mrp-1 protein. Translational fusion construct. pPD95.75_Pmrp-1::mrp-1(isoform C cDNA)::gfp.DepositorInsertMRP-1 (mrp-1 Nematode)
UseTagsGFPExpressionWormMutationPromoterPmrp-1Available sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJL1
Plasmid#184129PurposeFor rescuing null mrp-1 mutation. pPD95.77_Pmrp-1::mrp-1(isoform C cDNA)::SL2::gfp. SL2 prevents fusion of MRP-1 and GFP.DepositorInsertMRP-1 (mrp-1 Nematode)
UseTagsGFPExpressionWormMutationPromoterPmrp-1 (3kb)Available sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shPrc1
Plasmid#160959PurposePrc1 shRNA in pMKO.1 retroviral vectorDepositorInsertPrc1 shRNA (Prc1 Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterU6Available sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pICSL11023
Plasmid#117542PurposeLevel 1 Golden Gate Cassette: Cas9 expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + SpCas9-h (no stop codon)(pICSL90005) + YFP (pICSL50005)+ Nos (pICH41421)
UseTagsExpressionPlantMutationPromoter2x35S_OMEGA(pICH51288)Available sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
PcDNA-DEST47-CypB-Del3 C-term GFP
Plasmid#36136DepositorInsertcyclophilin B (PPIB Human)
UseTagsGFPExpressionMammalianMutationcontains amino acids 39-216 of cyclophilin B; G63…PromoterCMVAvailable sinceNov. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pResQ shDna2'
Plasmid#31952DepositorInsertshRNA against hDna2 (DNA2 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
TR- ATG-HP-GFP
Plasmid#80592PurposeExpresses GFP with an Csy4 target hairpin inserted immediately following the start codon for Csy4-mediated regulation. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.DepositorInsertGFP
UseAAVTagsExpressionMammalianMutationInserted 30 nt hairpin which is targeted by Csy4 …PromoterCBAAvailable sinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-PB1-MontiRed-LOV2dark
Plasmid#178874PurposeMammalian expression of PB1-MontiRed-LOV2darkDepositorInsertPB1-MontiRed-LOV2dark
UseTagsExpressionMammalianMutationLOV2: C450A, L514K, G528A, L531E, N538EPromoterCAGAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
WT (REP10)
Plasmid#21368DepositorInsertautosomal dominant polycystic kidney disease type I (PKD1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-ChRger1-TS-EYFP
Plasmid#127244PurposeHigh photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.DepositorInsertChRger1-TS-EYFP
UseAAVTagsEYFP and SpyTagExpressionMammalianMutationPromoterCamKIIaAvailable sinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
M48 GST-TEV-Chibby-N
Plasmid#17196DepositorInsertChibby (CBY1 Human)
UseTagsGSTExpressionBacterialMutationamino acids 1-63PromoterAvailable sinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLSU4mg10
Plasmid#126079PurposePlant transformation vector for stable expression of mitochondria targeted GFP1-10 in plant cellsDepositorInsertmitochondria Rieske iron sulphur protein (1-100 aa)
UseBinaryTagsGFP1-10ExpressionPlantMutationPromoter'long' CaMV35SAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
UR1Ct (265-1040)
Plasmid#115647Purposeexpression of truncated Rag1 including the RING, E3 ubiquitin ligase (265-1008) and C-terminal region (384-1040)DepositorInsertRag1 (265-1040) (Rag1 Mouse)
UseTags8XHis-MBP-PreScissionExpressionMammalianMutationtruncated Rag1 (aa 265-1040)PromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-2
Plasmid#172739PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-2; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-2
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET30b GluBP600n
Plasmid#119835PurposeProduction of recombinant GluBP600n high affinity protein in E. coliDepositorInsertGluBP600n
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only