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Plasmid#90469PurposeLentiviral vector expressing a doxycycline-inducible shRNA against human CUX1 sequence starting at 5150 of M74099DepositorInsertTGCTGTTGACAGTGAGCGTCAGAGCGATAATACACTATTATAGTGAAGCC
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry_LD 0_CS G_tTA-BFP
Plasmid#58856PurposeMESA target chain with mCherry ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with mCherry ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-8
Plasmid#172761PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-8; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-8
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-5
Plasmid#172760PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-5; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-5
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIS1 IMP-1 long UTR
Plasmid#21640DepositorInsertIGF-II mRNA-binding protein 1 UTR (IGF2BP1 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1V5-His Snk/Plk2 D223N
Plasmid#22218DepositorInsertSnk/Plk2 (PLK2 Human)
UseTagsExpressionMammalianMutationsingle amino acid substitution mutant, aspartic a…PromoterAvailable sinceAug. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pY007 (pcDNA3.1-hPeCpf1)
Plasmid#69979PurposeExpresses humanized PeCpf1DepositorInserthPeCpf1
UseCRISPRTagsNLS & 3xHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30a-SRAP
Plasmid#84524PurposeExpresses murine SRAP domain (250 aa) in bacteriaDepositorInsertSRAP domain (Hmces Mouse)
UseTags6xHis and S-TagExpressionBacterialMutationPromoterT7Available sinceNov. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pY008 (pcDNA3.1-hPbCpf1)
Plasmid#69980PurposeExpresses humanized LPbCpf1DepositorInserthPbCpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7 shRNA against CRTC1
Plasmid#72710PurposeLentiviral expression of shRNA against CRTC1 (3rd generation)DepositorInsertCRTC1
UseLentiviralTagsCMV-EGFPExpressionMammalianMutationPromoterU6Available sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
cycLuc_SbMVs
Plasmid#119209PurposeCircularly permutated firefly luciferase with SbMV cleavage site (Luciferase reporter for detection of proteolytic activity)DepositorInsertcyclic luciferase with SbMVp cleavage site
UseLuciferaseTagsAU1 tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-DARPinOFF7
Plasmid#182397PurposeBacterial expression of Vesicle Nucleating peptide-DARPinOFF7 fusionDepositorInsertVNp-DARPinOFF7
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
dTomato_LD 0_CS M_tTA-BFP
Plasmid#58875PurposeMESA target chain with dTomato ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with dTomato ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')
UseAAVTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
mouse Cavin-1
Plasmid#50441Purposeexpresses mouse Cavin-1 in mammalian cellsDepositorInsertCavin-1 (Cavin1 Mouse)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-V5-Vrk2
Plasmid#120240PurposeExpresses V5-tagged Vrk2 in lentiviral vectorDepositorInsertVrk2 (Vrk2 Mouse)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1X-Nup93-mut
Plasmid#87335PurposeTo express human Nup93 in mammalian cells. It contains synonymous mutations that are not targeted by shRNADepositorInsertNUP93 (NUP93 Human)
UseTagsEGFPExpressionMammalianMutationshRNA resistant changesPromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ shCUX1-5328
Plasmid#100815PurposeLentiviral vector expressing a doxycycline-inducible short hairpin RNA against CUX1 sequence starting at 5328 of M74099DepositorInsertTGCTGTTGACAGTGAGCGTCTTCTCGTTTGAAACTTTGAATAGTGAAGCCA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCin4-3xFLAG-MKL1 S449A/T450A/S454A
Plasmid#19845DepositorInsertMKL1 S449A/T450A/S454A (MRTFA Human)
UseTags3xFLAGExpressionMammalianMutationchanged Serine 449, Threonine 450 and Serine 454 …PromoterAvailable sinceDec. 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBS-tRNA-Ser
Plasmid#174318PurposeFor in vitro transcription of mouse tRNAsDepositorInserttRNA-ser
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGluf
Plasmid#119833PurposeProduction of recombinant iGluSnFR E25D fast variant, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGluf
UseTagsGSTExpressionBacterialMutationE25DPromoterT7Available sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only