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Plasmid#105013Purposemitochondrial calcium sensorDepositorInsertRCaMP1h
UseTagsExpressionMammalianMutationPromoterpCAGAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
RfxCas13d-backbone
Plasmid#165078PurposeExpression of RfxCas13d sgRNAs from a U6 promoterDepositorInsertScaffold gRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTECH-MmAcK3RS(IPYE)
Plasmid#104071PurposeExpression plasmid encoding MmAcK3RS(IPYE) and pylTDepositorInsertPlpp MmAcK3RS(IPYE); PproK pyl
UseTagsExpressionBacterialMutationContains activating mutations V31I, T56P, H62Y, A…PromoterPlpp and PproKAvailable sinceDec. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBUE411
Plasmid#62200PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistanceDepositorInsertsgRNA scaffold
zCas9
UsePlant binary vectorTags3x FLAG and NLSExpressionPlantMutationmaize codon optimizedPromoterOsU3 and UbiAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pmiRFP670-N1
Plasmid#79987PurposeMonomeric near-infrared fluorescent protein miRFP670 in mammalian plasmidDepositorHas ServiceCloning Grade DNAInsertmiRFP670
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-SARS-CoV-2-S-RBD-8his
Plasmid#145145PurposeMammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) with 8his tagDepositorInsertSecreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
UseTags8his tagExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMOS013: mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)
Plasmid#163056Purposemito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)DepositorInsertmito-roGFP2-Grx1
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP8m-WPRE
Plasmid#162375PurposeAAV-mediated expression of ultrafast protein calcium sensor under the Syn promoterDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9InsertjGCaMP8m
UseAAVTags6xHisExpressionMammalianMutationA25G F286YPromoterSynapsinAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUCsRNAP
Plasmid#182746PurposeSwapping in small RNA promoter, 23-bp spacer sequence, & 19-bp repeats into pJC005 plasmidsDepositorInsertsmall RNA promoter, a 23-bp spacer sequence, & 19-bp repeats
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
mRuby-N1
Plasmid#54581PurposeLocalization: N1 Cloning Vector, Excitation: 558, Emission: 605DepositorTypeEmpty backboneUseTagsmRubyExpressionMammalianMutationPromoterAvailable sinceJune 12, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
V5-miniTurbo-NES_pCDNA3
Plasmid#107170Purposeexpresses V5-tagged miniTurbo in the mammalian cytosolDepositorInsertminiTurbo (BirA mutant)
UseTagsExpressionMammalianMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD WT
Plasmid#169713PurposeBacterial expression of human hnRNPA1-A Low complexity domain including amino acids 186-320DepositorInserthnRNPA1 (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationhnRNPA1-A Low complexity domain including amino a…PromoterT7Available sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYL156 (TRV RNA2)
Plasmid#148969PurposeModified Tobacco Rattle Virus RNA 2; used for virus induced gene silencing in plants along with pYL192 (TRV RNA1)DepositorInsertModified TRV RNA2
UseT-dna vectorTagsExpressionMutationsee comments section belowPromoterAvailable sinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
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SIN40C.SFFV.Luciferase.IRES.GFP
Plasmid#169308PurposeConstitutive overexpression of Luciferase cDNA with GFP as a reporter fluorescent proteinDepositorInsertLuciferase
UseLentiviralTagsIRES-EGFPExpressionMutationPromoterSFFVAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HRE-dUnaG
Plasmid#124372PurposeUnaG fluorescent protein reporter for hypoxia-induced factor (HIF) signalingDepositorInsertHRE-dUnaG
UseLentiviralTagsPEST-degron and Myc tagExpressionMutationPromoterHRE (HIF responsive element 5x + CMV minimal prom…Available sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-FAK WT
Plasmid#186148PurposeFor expressing FAK in mammalian cellsDepositorInsertPTK2 (PTK2 Mouse)
UseTagsmEGFPExpressionMammalianMutationPromotercmvAvailable sinceJuly 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-NLS-dCas9-HA-2XNLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-WPRE-PolyA-PGK-EM7-NeoR-polyA
Plasmid#168290PurposeCRISPR-activator Suntag-P65-HSF1 (SPH)DepositorInsertdCas9, Suntag-P65-HSF1
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP
Plasmid#118156PurposeCatalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.DepositorInsertKRAB-dCas9-P2A-EGFP
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterEF1a core and U6Available sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-FLEX-FLIM-AKAR
Plasmid#60445PurposeExpresses the Fluorescence Lifetime Imaging Microscopy (FLIM)-compatible PKA activity sensor FLIM-AKAR in a Cre dependent manner in an AAV backboneDepositorInsertFLIM-AKAR
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits only