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pKLV2-U6gRNA5(Tcf7l1-g2)-PGKpuroBFP-W

Plasmid

#105016

Purpose

Lentiviral gRNA plasmid targeting mouse Tcf7l1 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Tcf7l1 (Tcf7l1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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Sdk2-Fc-His

Plasmid

#72135

Purpose

Expresses the extracellular region of the Sdk2 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sdk2 (Sdk2 Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pUCM-AAVS1-TO-hNGN2

Plasmid

#105840

Purpose

Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron

Depositor

Michael Ward

Article

Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

Inserts

Neurogenin 2 (NEUROG2 Human)
mCherry
rtTA3G

Use

CRISPR and TALEN

Tags

Expression

Mutation

Promoter

CAG, EF-1alpha, and TRE3G

Available since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti6.3 GCN1-3xFlag_IRES-iRFP

Plasmid

#198388

Purpose

GCN1 expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

GCN1 (GCN1 Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 CB1

Plasmid

#13391

Purpose

Expresses mouse cannabinoid receptor 1 (CB1) in mammalian cells

Depositor

Mary Abood

Article

Abood et al Biochem Pharmacol. 1997 Jan 24. 53(2):20

Insert

CB1 (Cnr1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2006

Availability

Academic Institutions and Nonprofits only

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pHR RPS27A K107/113R-3xFlag_IRES-GFP

Plasmid

#198392

Purpose

RPS27A K107/113R (ubiquitin/ribosomal protein eS31 fusion) expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

RPS27A (RPS27A Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

ubiquitination site double mutant (K107/113R)

Promoter

EF1A

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pLX304 3xFlag-eRF1_IRES-GFP

Plasmid

#198393

Purpose

eRF1 expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

eRF1 (ETF1 Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 3xFlag IFIT2

Plasmid

#53555

Purpose

mammalian expression of IFIT2

Depositor

Kathleen Collins

Article

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT2 (IFIT2 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pMTTH+mEGFP-H1.4

Plasmid

#157799

Purpose

Expression of mEGFP and human linker histone H1.4 fusion protein

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

mEGFP-H1.4 (H1-4 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

pTAC

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV CytoBAS

Plasmid

#62860

Purpose

Fluorescent bile acid sensor (BAS) that allows spatiotemporal monitoring of bile acid transport in single living cells using FRET

Depositor

Stan van de Graaf

Article

van der Velden et al Hepatology. 2013 Feb;57(2):740-52. doi:

Insert

CytoBAS

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV NucleoBAS

Plasmid

#62861

Purpose

Fluorescent bile acid sensor (BAS) targeted to nucleus that allows spatiotemporal monitoring of bile acid transport in single living cells using FRET

Depositor

Stan van de Graaf

Article

van der Velden et al Hepatology. 2013 Feb;57(2):740-52. doi:

Insert

NucleoBAS

Use

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a(+) 6xHis-TEV-IFIT2

Plasmid

#53558

Purpose

bacterial expression of IFIT2

Depositor

Kathleen Collins

Article

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT2 (IFIT2 Human)

Use

Tags

6xHIS and TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only
anxA10-GFP

Plasmid

#107199

Purpose

Expresses human annexin A10-GFP fusion protein for live cell imaging

Depositor

Ursula Rescher, Volker Gerke

Article

Quiskamp et al Cell Mol Life Sci. 2014 Jan;71(2):311-29

Insert

annexin A10 (ANXA10 Human)

Use

Tags

Green Fluorescent Protein

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) mouse Egr2

Plasmid

#107997

Purpose

Expresses mouse Egr2 in mammalian cells

Depositor

Peter Johnson

Article

Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

Insert

Egr2 (Egr2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ULK2

Plasmid

#97075

Purpose

Expresses ULK2 in mammalian cells

Depositor

Pasquale Vito

Article

Scudiero et al Cell Death Dis. 2017 Feb 23;8(2):e2627.

Insert

ULK2 (ULK2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Nprl2-g1)-PGKpuroBFP-W

Plasmid

#105037

Purpose

Lentiviral gRNA plasmid targeting mouse Nprl2 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Nprl2 (Nprl2 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-U6gRNA5(Nprl2-g2)-PGKpuroBFP-W

Plasmid

#105038

Purpose

Lentiviral gRNA plasmid targeting mouse Nprl2 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Nprl2 (Nprl2 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
TMED2-bio-His

Plasmid

#51853

Purpose

Expresses full-length Transmembrane emp24 domain-containing protein 2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

TMED2 (TMED2 Human)

Use

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 RL23Cm6

Plasmid

#109367

Purpose

Human rod opsin chimera with intracellular loops 2-3 and C-terminus from mGluR6

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Rod opsin-mGluR6 chimera (RHO, GRM6 Human)

Use

Tags

1D4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Vps39-g1)-PGKpuroBFP-W

Plasmid

#105019

Purpose

Lentiviral gRNA plasmid targeting mouse Vps39 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Vps39 (Vps39 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge