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Plasmid#137926PurposeR0SA26 knock-in vector with microhomology-mediated end-joining(MMEJ)-based methodDepositorInsertSA-EGFP-bpA with micro-homology arms
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
p2xFlag CMV2-YAP2-deltaC
Plasmid#21123DepositorArticleInsertYes-kinase associated protein (YAP1 Human)
UseTags2xFlagExpressionMammalianMutation5 Carboxyterminal amino acids -FLTWL are deletedPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pY006 (pcDNA3.1-hBpCpf1)
Plasmid#69978PurposeExpresses humanized BpCpf1DepositorInserthBpCpf1
UseCRISPRTagsNLS & 3xHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-ChRger2-TS-EYFP
Plasmid#127238PurposeHigh photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.DepositorInsertChRger2-TS-EYFP
UseAAVTagsEYFP and SpyTagExpressionMammalianMutationPromoterCamKIIaAvailable sinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
3495 pcDNA3 Bid p15
Plasmid#8780DepositorInsertBid p15 (Bid Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pY013 (pcDNA3.1-hEeCpf1)
Plasmid#69985PurposeExpresses humanized EeCpf1DepositorInserthEeCpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS415-Cas9-VPR
Plasmid#163971PurposeTrifunctional Cas9-VPR fusion protein plasmid for simultaneous transcriptional activation, transcriptional repression, and genome editing in yeastDepositorInsertCas9-VPR
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3HA-AMOT p130∆PPxY1
Plasmid#166711Purposeexpresses AMOT with inactivating mutation in first PPxY motif in mammalian cellsDepositorInsertAngiomotin p130 L129A P130A
UseTags2xHAExpressionMammalianMutationchanged leucine 129 to alanine, proline 130 to al…PromoterCMV promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAYC
Plasmid#62657PurposeMammalian expression of iCre recombinase from the broadly active CAG promoter/enhancerDepositorInsertiCre
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pY019 (pcDNA3.1-hPmCpf1)
Plasmid#69991PurposeExpresses humanized PmCpf1DepositorInserthPmCpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pY009 (pcDNA3.1-hSsCpf1)
Plasmid#69981PurposeExpresses humanized SsCpf1DepositorInserthSsCpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pICSL80036
Plasmid#68259PurposeLevel 0 Golden Gate Part: Coding sequence, hygromycin phophotransferase II (Escherichia coli)DepositorInsertCoding sequence, hygromycin phophotransferase II (Escherichia coli)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
dTomato_LD 0_CS G_tTA-BFP
Plasmid#58871PurposeMESA target chain with dTomato ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with dTomato ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-24
Plasmid#172750PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-24; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-24
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-9
Plasmid#172742PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-9; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-9
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pY015 (pcDNA3.1-hLiCpf1)
Plasmid#69987PurposeExpresses humanized LiCpf1DepositorInserthLiCpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7 shRNA against CRTC3
Plasmid#72712PurposeLentiviral expression of shRNA against CRTC3 (3rd generation)DepositorInsertCRTC3
UseLentiviralTagsCMV-EGFPExpressionMammalianMutationPromoterU6Available sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
3xEGFP-mMeikin (E156R, R159E)
Plasmid#174710PurposeMammalian expression of mouse Meikin with Separase-resistant mutationsDepositorInsertMeikin (Meikin Mouse)
UseTags3xEGFPExpressionMammalianMutationE156R, R159EPromoterAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNOC-CRISPR-35S
Plasmid#99861PurposeExpresses Cas9-Nlux and sgRNA. Contains CEN/ARS and 35 terminator. No selection marker.DepositorTypeEmpty backboneUseAlgae, nannochloropsis expressionTagsCas9-NluxExpressionMutationPromoterRibi promoterAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -