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Plasmid#183963PurposeCBA-hyperdCas12a-HA-KRAB-mCherryDepositorInsertHyperdCas12a
UseCRISPRTagsHA, KRAB, and mCherryExpressionMammalianMutationD832A, D156R, E292R, D235R, D350RPromoterCBAAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-RAM-d2TTA::TRE-FLEX-tdTomato-WPREpA
Plasmid#84468PurposepAAV-CRAM-tdTomatoDepositorInserttdTomato
UseAAVTagsExpressionMutationT230I (please see depositor comments below)PromoterTREAvailable sinceDec. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWB442
Plasmid#74828PurposeGateway cloning compatible binary vector for N-terminal fusion with eYFP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-pAzF[TAG]
Plasmid#164579PurposeMachinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codonsDepositorInsertsMj pCNFRS[TAG] (aaRS1)
Mj pCNFRS[TAG] (aaRS2)
Mj tRNA[TAG]
UseTagsExpressionBacterialMutationY32L L65V F108W Q109M D158G I159PPromoteraraBAD, glnS, and proKAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCFJ601 - Peft-3 Mos1 transposase
Plasmid#34874PurposeMos1 transposase expression vector for use in creating targeted transgene insertions and targeted deletions in C. elegansDepositorInsertMos1 transpoase
UseWorm targetingTagsExpressionMutationPromotereef-1A.1 (eft-3)Available sinceFeb. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET14b – E. coli Exonuclease I
Plasmid#104552Purposebacterial expression of Exonuclease IDepositorInsertExonuclease I (sbcB E.coli)
UseTags6X-His tag followed by thrombin siteExpressionBacterialMutationPromoterT7Available sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
GPRC5A-Tango
Plasmid#66382PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertGPRC5A (GPRC5A Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
TRE-UBC-HLA-E SCT-T2A-GFP
Plasmid#205461Purposelentiviral plasmid for expression of HLA-E SCTDepositorInsertHLA-E SCT (HLA-E Human)
UseTagsExpressionMammalianMutationPromoterUBCAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV Ef1a-EGFP-WPRE
Plasmid#135428PurposeCan be used to express EGFP. Can also be used to create adeno-associated virus for delivery of the EGFP sequenceDepositorInsertEGFP
UseAAVTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
YEp195-CUP1-His-Ubi
Plasmid#99540PurposeYeast episomal vector for expression of His-tagged ubiquitin; URA3 markerDepositorInsertUBI3(1-76) (RPL40A Budding Yeast)
UseTags7xHisExpressionYeastMutationPromoterCUP1Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pB-CAGGS-dCas9
Plasmid#110823PurposePiggyBac compatible plasmid expressing dCas9DepositorInsertdCas9
UseTagsExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCAGGSAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMBP-FnCas12a
Plasmid#113432PurposeExpresses 10xHis-MBP fused to FnCas12aDepositorInsertFnCas12a
UseTagsMBPExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Matr3 delRRM1
Plasmid#32881DepositorInsertMATR3 Matrin3 (MATR3 Human)
UseTagsFLAGExpressionBacterial and MammalianMutationdeleted amino acids 399-468PromoterCMVAvailable sinceJan. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHJ12: CadC-VHH-Caffeine (No linker)
Plasmid#108244PurposeMembrane bound transcription activator CadC fusing VHH-Caffeine. Upon ligand binding, CadC-VHH-Caffeine can be dimerized and activate down stream reporter gene sfGFP expressionDepositorInsertMembrane bound transcription activator CadC fusing with VHH-Caffeine antibody without external linker
UseSynthetic BiologyTagsExpressionMutationPromoterpLacO1Available sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTHSSe_60
Plasmid#109254PurposeTALEsp2 stabilized promoter driving GFPDepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterTALEsp2 stabilized promoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUBC-SV40NLS-Pfv-Sapphire
Plasmid#203653PurposeMammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter.DepositorArticleInsertPfV
UseLentiviralTagsSapphireExpressionMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSEM246 - MCS mosTI unc-119 target
Plasmid#159821PurposeSingle copy or array insertion by MosTI using split unc-119 selectionDepositorInsertMCS mosTI unc-119 target
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-EF1Alpha-Tet-on 3G
Plasmid#118592PurposeExpresses Tet-on 3G from the EF1Alpha promoterDepositorInsertTet-on 3G
UseLentiviralTagsExpressionMammalianMutationPromoterEF1AlphaAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC101-pT7-T3RNAP
Plasmid#140872PurposeT7 promoter driven synthesis of T3 RNA polymeraseDepositorInsertT3 RNA polymerase
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXF87
Plasmid#139027PurposeMosSCI backbone for MosSCI-SapTrap assemblyDepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOD1988-epiDEG
Plasmid#89357PurposeMosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pdpy-7 (epidermis specific).DepositorInsertvhhGFP4-ZIF-1 (zif-1 Synthetic, Nematode)
UseTargeting vector for mos1 transposon mediated sin…TagsvhhGFP4 (GFP nanobody)ExpressionMutationPromoterPdpy-7Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPGC1a_Proximal Promoter-FLuc2P
Plasmid#154259PurposeDestabilized firefly luciferase reporter containing the Human PGC-1alpha Proximal Promoter from -500 to +52.DepositorInsertPGC-1a Proximal Promoter (PPARGC1A Human)
UseTagsExpressionMammalianMutationPromoterHuman PGC-1alpha Proximal PromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
FCK-ChR2-GFP
Plasmid#15814PurposeLentiviral expression of ChR2-GFP for neuronal excitationDepositorInsertChR2
UseLentiviralTagsGFPExpressionMammalianMutationPromoterCaMKIIAvailable sinceOct. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV GFP DEST (736-1)
Plasmid#19732Purpose3rd gen lentiviral Gateway destination vector, expression/RNAi, eGFP co-expressionDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral and RNAi; Gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJF6
Plasmid#139040PurposeMosSCI SapTrap assembly: Cassette 3 for 4-cassette systemDepositorInsertgfp (includes STOP codon, PATC introns)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-N-Myc_535-888 USP7
Plasmid#131248PurposeMammalian expression of the USP7 UBL1-3 domains with an N-terminal Myc tag.DepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
UseTagsMycExpressionMammalianMutationA mutant form of our pcDNA3.1-N-Myc_535-1102 USP7…PromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-N-Myc_884-1102 USP7
Plasmid#131249PurposeMammalian expression of the USP7 UBL4-5 domains with an N-terminal Myc tagDepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
UseTagsMycExpressionMammalianMutationEncodes USP7 UBL domains 4-5 (USP7 amino acids 88…PromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cre Stoplight
Plasmid#45150DepositorInsertCre Stoplight
UseTagsDsRed and EGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
N1-Tq-Ca-FLITS
Plasmid#191454PurposeGenetically encoded calcium sensor (GECI) Tq-Ca-FLITS that reports with lifetime and intensity changesDepositorInsertTq-Ca-FLITS
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-beta-catenin deltaN90
Plasmid#36985DepositorInsertbeta-catenin deltaN90 (CTNNB1 Human)
UseLentiviralTagsMycExpressionMammalianMutationDeletion of amino acids 1-90 (Constitutively Acti…PromoterEF1aAvailable sinceSept. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-EGFP-NLS
Plasmid#86677PurposeExpresses nuclear-localized GFP after lentiviral transduction. Can be used to monitor GFP leakage into the cytosol following nuclear envelope rupture events.DepositorInsertEGFP-NLS
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDAC569
Plasmid#195241PurposeMammalian expression of Csm complex (RNase mut) from separate promoters; GFP backbone (Used for RNA binding/tethering)DepositorInsertFLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3(RNase mut); FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
p273 EF1a_mCherry2_hH11
Plasmid#164852PurposemCherry2-expression vector for human H11 locusDepositorInsertmCherry2
UseTagsExpressionMammalianMutationPromoterEF1-alphaAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
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GFP-PKCA WT
Plasmid#195202PurposeMammalian expression vector for GFP tagged PKCA.DepositorInsertPRKCA (PRKCA Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRC/CMV-JAK1-VSV
Plasmid#139356PurposeExpression of JAK1 proteinDepositorInsertJAK1 cDNA with 300nt of 5'UTR (JAK1 Human)
UseTagsVSVExpressionMammalianMutationPromoterCMVAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
7xSMAD_RE::FLuc
Plasmid#124531PurposeSMAD2 FLuc luciferase reporterDepositorInsertSMAD FLuc reporter
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ERT2-PhoCl-Gal4VP16-PhoCl-ERT2
Plasmid#87693PurposeExpress ERT2-PhoCl-Gal4VP16-PhoCl-ERT2 in mammalian cellsDepositorInsertERT2-PhoCl-Gal4VP16-PhoCl-ERT2
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pI1-MTU-DO-HPH
Plasmid#160014PurposeIntegrative vector for K. marxianus, targeting the LAC4 locus. Hygromycin selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDT386
Plasmid#174733PurposeExpresses His-tagged DT386, a truncated diphtheria toxin without a receptor-binding domain.DepositorInsertDT386
UseTagsHistagExpressionBacterialMutationDeleted amino acids 412-560 (the receptor binding…PromoterT5Available sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
multi-shRNA vector
Plasmid#12391DepositorInsertmSOD2 shRNA (Sod2 Mouse)
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
CMV-hEAAT2
Plasmid#32814DepositorInserthEAAT2 (SLC1A2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pQE 80L Kan T7WT
Plasmid#102790PurposeExpresses T7 RNA polymeraseDepositorInsertT7 RNA polymerase
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 3, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGSKU
Plasmid#72243PurposeContains the CORE cassette with convergent KlURA3 and KanMX4 markers and the I-SceI gene under the inducible GAL1 promoterDepositorInsertsKlURA3
kanMX4
I-SceI
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pBiFC-bJunVN155(I152L)
Plasmid#27098DepositorInsertbJunVN155(I152L) (Jun Rat, and Aequorea Victoria)
UseTagsMycExpressionMammalianMutationI152L in Venus(1-154)PromoterAvailable sinceJan. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-nbMmIgG1-Tn5
Plasmid#184287PurposeAnti-mouse IgG1 Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)DepositorInsertnbMmIgG1-Tn5
UseTagsExpressionMutationPromoterAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
BirA-Myc_N_MYC
Plasmid#167715PurposeExpresses MYC in mammalian cellsDepositorInsertMYC (MYC Human)
UseTagsMyc-BirAExpressionMammalianMutationno mutationsPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
Mitochondrial cpGFP
Plasmid#215711PurposeExpresses cpGFP in the mitochondriaDepositorInsertcpGFP
UseLentiviralTagsFLAG, HA, and mitochondrial localization sequence…ExpressionMammalianMutationcircularly permuted GFPPromoterCMVAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBABE-zeo smallT
Plasmid#8583DepositorInsertSV40 small T Antigen (SV40gp7 simian virus)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits only