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Plasmid#215753PurposeExpresses binding-deficient K299E/D333A mutant of 2xDNMT3A PWWP domain fused to C-terminal part of IFP2.0 as negative control for H3K36me2/3 detection in BiAD sensorsDepositorInsert2x3APWWP_K299E_D333A-IFP2.0C
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV_NFL(K468TAG)-FLAG
Plasmid#182658PurposeExpresses mouse neurofilament light chain with a TAG codon at the position K468 and a C-terminal FLAG tag (DYKDDDDK) in mammalian cells.DepositorInsertmouse neurofilament light chain with a K468TAG mutation (Nefl Mouse)
UseTagsFLAG tag (DYKDDDDK)ExpressionMammalianMutationK468TAGPromoterCMVAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_NFL(K211TAG)-FLAG
Plasmid#182655PurposeExpresses mouse neurofilament light chain with a TAG codon at the position K211 and a C-terminal FLAG tag (DYKDDDDK) in mammalian cells.DepositorInsertmouse neurofilament light chain with a K211TAG mutation (Nefl Mouse)
UseTagsFLAG tag (DYKDDDDK)ExpressionMammalianMutationK211TAGPromoterCMVAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_NFL(R438TAG)-FLAG
Plasmid#182657PurposeExpresses mouse neurofilament light chain with a TAG codon at the position R438 and a C-terminal FLAG tag (DYKDDDDK) in mammalian cells.DepositorInsertmouse neurofilament light chain with a R438TAG mutation (Nefl Mouse)
UseTagsFLAG tag (DYKDDDDK)ExpressionMammalianMutationR438TAGPromoterCMVAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMIND
Plasmid#24730DepositorInsertTetRO promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-LEU2MX6
Plasmid#33138Purposegene deletion in S. cerevisiae and S. pombeDepositorInsertLEU2MX6
UseTagsExpressionMutationPromoterAvailable sinceJan. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-GFP-ura4MX6
Plasmid#33140Purposegenomic GFP tagging in yeastDepositorInsertsura4MX6
GFP
UseTagsExpressionMutationPromoterAvailable sinceJan. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Fis1-DTM
Plasmid#191950PurposeExpression of a Fis1-GFP chimera lacking the C-terminal 27 residues (Fis1-DTM)DepositorInsertFis1-DTM
UseTagsExpressionBacterialMutationFis1 gene lacking the C-terminal 27 residuesPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGMCKq26-10M-hyg
Plasmid#27337DepositorInserttetR(sB), hygR
UseGateway destination vectorTagsExpressionMutationN/APromoterAvailable sinceAug. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSMPUW_8.1_ M2_CAMKIIA-gateway-V5
Plasmid#128461Purposelentiviral destination vector with CAMKIIA promoter and C-terminal V5 tagDepositorInsertCAMKIIA promoter and C-terminal V5 tag
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
7TGP
Plasmid#24305DepositorInsert7xTcf-eGFP // SV40-PuroR
UseLentiviralTagsExpressionMutationEGFP is GFP with S65T and F64L mutationsPromoterAvailable sinceApril 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
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R777-E288 Hs.ROS1-nostop
Plasmid#70572PurposeGateway ORF clone of human ROS1 [NM_002944.2] without stop codon (for C-terminal fusions)DepositorInsertROS1 (ROS1 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
3` CC: Hygro – GFP-N – loxP – mScarlet
Plasmid#219564Purpose3` circularization cassette.To induce Cre-mediated circularization or inversion of a genomic region with concomitant GFP reconstitution and mScarlet expression from the chromosomeDepositorInsert3` CC: Hygro – GFP-N – loxP – mScarlet
UseUnspecifiedTagsExpressionMutationPromoterEF1-aAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBPZpGAL4DBDUw
Plasmid#26233DepositorInsertZip-GAL4DBD
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
lenti-sgRNA puro
Plasmid#104990PurposeThis 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and puromycin resistance from an EF-1a promoter.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
lenti-sgRNA hygro
Plasmid#104991PurposeThis 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and hygromycin resistance from an EF-1a promoter.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
3` CC: Hygro – H2B-GFP-N – loxP – mScarlet
Plasmid#219565Purpose3` circularization cassette to induce Cre-mediated circularization of a genomic region flanked by loxP sites with H2B-GFP reconstitution and mScarlet expression from the chromosomeDepositorInsertHygroR_2A_H2B-GFP-N_loxP_mScarlet
UseUnspecifiedTagsExpressionMutationPromoterEF-1aAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
loxP-Hygro-loxP-HA-mStayGold-Rab11 HR
Plasmid#229678PurposeHomology repair plasmid for endogenous tagging of Rab11 at the N-terminus with monomeric StayGold and an HA epitope tag. Contains a hygromycin resistance cassette for selection of edited cellsDepositorInsertgenomic sequence Rab11 locus (RAB11A Human)
UseCRISPRTagsmonomeric StayGold, HAExpressionMutationPromoterAvailable sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only