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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 21232 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHACE
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePKC alpha
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Alt nameprotein kinase C, alpha
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SpeciesH. sapiens (human)
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Insert Size (bp)2019
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Entrez GenePRKCA (a.k.a. AAG6, PKC-alpha, PKCA, PKCI+/-, PKCalpha, PRKACA)
- Promoter CMV
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Tag
/ Fusion Protein
- HA (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PKC alpha WT was a gift from Bernard Weinstein (Addgene plasmid # 21232 ; http://n2t.net/addgene:21232 ; RRID:Addgene_21232) -
For your References section:
Roles of specific isoforms of protein kinase C in the transcriptional control of cyclin D1 and related genes. Soh JW, Weinstein IB. J Biol Chem. 2003 Sep 5. 278(36):34709-16. 10.1074/jbc.M302016200 PubMed 12794082