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Showing: 9321 - 9360 of 156554 results

  3. pNH-TrxT

    Plasmid
    #26106
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Opher Gileadi
    Article
    Savitsky et al J Struct Biol. 2010 Jun 10. ():.
    Type
    Empty backbone
    Use
    Tags
    6xHis, TEV cleavage site, and TrxA
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    Dec. 2, 2010
    Availability
    Academic Institutions and Nonprofits only
    26106-plasmid-map-sequence-id-239406

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  8. PRMT8-FLAG

    Plasmid
    #164699
    Purpose
    Expression of PRMT8 with a C-terminal FLAG tag
    Depositor
    Cheryl Arrowsmith
    Insert
    PRMT8 (PRMT8 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    164699-plasmid-map-sequence-id-326674

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  11. pICE ADE2

    Plasmid
    #122876
    Purpose
    ADE2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.
    Depositor
    Randy Hampton
    Article
    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340
    Type
    Empty backbone
    Use
    Yeast recombination cloning
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    122876-plasmid-map-sequence-id-238615

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  17. NRXN-bio-His

    Plasmid
    #51810
    Purpose
    Expresses full-length Neurexin-1-alpha precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    NRXN (NRXN1 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51810-plasmid-map-sequence-id-79534

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  18. pPB-US-ECasE

    Plasmid
    #83961
    Purpose
    Piggyac transposon containing tamoxifen inducible Cas9 and a gRNA cassette.
    Depositor
    Eleanor Chen
    Insert
    ERT2-spCas9-ERT2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    please see depositor comments below
    Promoter
    U6 (gRNA), EF1a (ERT2-Cas9-ERT2)
    Available since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    83961-plasmid-map-sequence-id-214594

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  22. p-dCas9-JMJD2A-Hygro

    Plasmid
    #104410
    Purpose
    transient expression of dCas9-JMJD2A fusion protein
    Depositor
    Stephan Beck
    Insert
    JMJD2A (KDM4A Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104410-plasmid-map-sequence-id-204464

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  24. AHSG-bio-His

    Plasmid
    #52175
    Purpose
    Expresses full-length Alpha-2-HS-glycoprotein precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    AHSG (AHSG Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52175-plasmid-map-sequence-id-80539

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  27. pCMV-PE6f

    Plasmid
    #207856
    Purpose
    Mammalian expression of PE6f prime editor
    Depositor
    David Liu
    Article
    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.
    Insert
    PE6f
    Use
    Tags
    SV40 bpNLS and SV40 bpNLS, c-Myc NLS
    Expression
    Mammalian
    Mutation
    Cas9(H840A, E471K, H99R, I632V, H721Y, D645N, K91…
    Promoter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    207856-plasmid-map-sequence-id-408168

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  29. pLEX_305

    Plasmid
    #41390
    Purpose
    Constitutive lentiviral expression, SV40-puro; PGK-gateway-no tag
    Depositor
    David Root
    Type
    Empty backbone
    Use
    Lentiviral; Gateway destination vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK
    Available since
    Nov. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    41390-plasmid-map-sequence-id-352470

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  30. pQ-Elo-HB-C-A(1-630)

    Plasmid
    #171086
    Purpose
    Co-expresses of ΔC-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOB
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Inserts
    Elongin B (ELOB Human)
    Elongin C (ELOC Human)
    Elongin A (ELOA Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Deleted N-term 26aa and C-term 142aa for mutant e…
    Promoter
    T5 promoter
    Available since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171086-plasmid-map-sequence-id-335555

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  37. pETcon(-)

    Plasmid
    #41522
    Depositor
    Andrew Scharenberg
    Type
    Empty backbone
    Use
    Tags
    3x(G4S) Linker, G3S Linker, HA, Myc Tag, and Stuf…
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    April 23, 2013
    Availability
    Academic Institutions and Nonprofits only
    41522-plasmid-map-sequence-id-59598

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Showing: 9321 - 9360 of 156554 results