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pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-A10

Plasmid

#196079

Purpose

Constitutive CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10.

Depositor

Joanna Bereta

Article

Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

Insert

gRNA targeting Adam10

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Cloned using SapI sites - destroyed after inserti…

Promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-A10

Plasmid

#196085

Purpose

Inducible CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10.

Depositor

Joanna Bereta

Article

Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

Insert

gRNA targeting Adam10

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Cloned using SapI sites - destroyed after inserti…

Promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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UAS-dsarm(E)

Plasmid

#187870

Purpose

Gal4/UAS expression of dSarm(isoE)

Depositor

Lukas Neukomm

Article

Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

Insert

dSarm isoform E (Sarm Fly)

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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ADAM10_Halo_C_allele

Plasmid

#178151

Purpose

Donor vector for endogenous tagging of human ADAM10 at the C-terminus with halotag

Depositor

Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

Insert

Halotag (ADAM10 Human)

Use

Donor vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-f1(-)-GAPDH-IRES-EGFP

Plasmid

#115918

Purpose

donor to insert IRES-EGFP at the GAPDH locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

IRES-EGFP

Use

Donor template

Tags

dTomato

Expression

Mutation

Promoter

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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MP-K13

Plasmid

#69657

Purpose

Untargetted Bacterial Mutagenesis

Depositor

David Liu

Article

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

dnaQ926 dam seqA polB

Use

Tags

Expression

Bacterial

Mutation

Promoter

E. coli PBAD

Available since

Oct. 13, 2015

Availability

Academic Institutions and Nonprofits only

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MP-P7

Plasmid

#69666

Purpose

Untargetted Bacterial Mutagenesis

Depositor

David Liu

Article

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

dnaQ926 dam seqA emrR polB

Use

Tags

Expression

Bacterial

Mutation

Promoter

E. coli PBAD

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pFRT_TO_DESTFLAGHA

Plasmid

#26361

Depositor

Thomas Tuschl

Article

Spitzer et al Genes Chromosomes Cancer. 2011 May . 50(

Type

Empty backbone

Use

Tags

FLAG/HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only
lentiCRISPR_DKO

Plasmid

#183192

Purpose

Plasmid with Cas9, two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen

Depositor

Lang Li

Article

Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6, mU6

Available since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pICH47761

Plasmid

#48003

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pSL3402 (pTnsABf_Pse)

Plasmid

#200901

Purpose

Expresses human codon-optimized Pseudoalteromonas TnsA-TnsB fusion from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

PseTnsA-TnsB

Use

Tags

Internal NLS between TnsA-TnsB fusion

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pLPT234

Plasmid

#127855

Purpose

Repressilator 2.0, without degradation tags, integrated triple reporter

Depositor

Johan Paulsson

Article

Riglar et al Nat Commun. 2019 Oct 11;10(1):4665. doi:

Inserts

bla
mSCFP3
mKate2
mVenus
TetR
LacI
cI

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

First 11aa derived from mCherry as fusion protein

Promoter

pAmpR, pL LacO1, pL tetO1, and pR

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pSLS.436

Plasmid

#184953

Purpose

Express -Eco1 RT and ncRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1: ncRNA(wt) and RT

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBbS8c-ddcpf1-Δ

Plasmid

#153039

Purpose

For CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array lacking a gene-specific spacer.

Depositor

Nigel Scrutton

Article

Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

Insert

Ascpf1

Use

Tags

Expression

Bacterial

Mutation

E993A

Promoter

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pREDIT_Cas9-MS2-BB_BbsI

Plasmid

#164802

Purpose

REDIT backbone for wildtype Cas9, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9-T2A-EBFP

Depositor

Le Cong

Article

Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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1533 pcDNA3 HA MARS

Plasmid

#10716

Depositor

William Sellers

Insert

MARS (MARS1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only

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pGWB406

Plasmid

#74800

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with sGFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Oct. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pBlu/gRNA

Plasmid

#59188

Purpose

Serves as a shuttle vector for target oligo before insertion into Cas9 destination vector

Depositor

Robert Stupar

Article

Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10

Insert

Guide RNA Cassette

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

U6 (Arabidopis)

Available since

Feb. 24, 2015

Availability

Academic Institutions and Nonprofits only

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plenti.CamKII.(synapto).cpsfGFP.miRFP670nano3

Plasmid

#214926

Purpose

Expresses presynaptic non-responsive control

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(synapto).cpsfGFP.miRFP670nano3

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CamKII

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-BaLgp120-8his

Plasmid

#100925

Purpose

Mammalian expression plasmid for gp120 subunit from the BaL HIV-1 strain

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env

Use

Tags

CD5 leader sequence and Purification tag after K5…

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pGEMT-Mef2c-tPT2A-GFP

Plasmid

#111771

Purpose

Bicistronic construct: Co-express two genes by 2A peptides

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Insert

Mef2c-tPT2A-GFP (Mef2c Mouse)

Use

Cloning intermediate

Tags

GFP

Expression

Mutation

Promoter

T7

Available since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.GCaMP6f.WPRE.SV40

Plasmid

#100836

Purpose

AAV expression of ultrasensitive protein calcium sensor from CAG promoter

Depositor

GENIE Project, Douglas Kim

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Has Service

AAV1, AAV2, and AAV9

Insert

GCaMP6f

Use

AAV

Tags

Expression

Mammalian

Mutation

GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

Promoter

CAG

Available since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pLX304 Luciferase-V5 blast

Plasmid

#98580

Purpose

Lentiviral vector for constitutive expression of Luciferase with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

Luciferase

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pCA_e-2

Plasmid

#202097

Purpose

Receiver cloning vector for even-2 level constructs. Contains lacZ for blue/white screening.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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WT GBA pcDNA3.1

Plasmid

#188580

Purpose

Ectopic expression of wild-type GBA

Depositor

Anthony Schapira

Article

Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

Insert

GBA (GBA1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TFEBS122D-MYC (#777)

Plasmid

#99956

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Autophagy. 2017 Mar 4;13(3):464-472. doi

Insert

TFEB (TFEB Human)

Use

Tags

6xHis and Myc

Expression

Mammalian

Mutation

S122D

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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p426-SNR52p-gRNA.CAN1.Y-SUP4t

Plasmid

#43803

Purpose

Encodes a gRNA that targets Can1.Y in yeast.

Depositor

George Church

Article

Dicarlo et al Nucleic Acids Res

Insert

CAN1.y gRNA (CAN1 Budding Yeast)

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

SNR52

Available since

March 21, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_Ado1.0med

Plasmid

#153286

Purpose

Express the medium-affinity adenosine sensor GRAB_Ado1.0med in neurons

Depositor

Yulong Li

Article

Wu et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Adenosine sensor GRAB_Ado1.0med (medium affinity)

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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FUGW-APOE-E3-T2A-mCherry

Plasmid

#190808

Purpose

Express APOE E3 allele from Ubiquitin C promoter, co-expressing mCherry

Depositor

Ronald Hart

Insert

APOE (APOE Human)

Use

Lentiviral

Tags

Expression

Mutation

E3

Promoter

Ubi C

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pKD279.8

Plasmid

#173171

Purpose

Encodes expression of SO_4388(REC)-PsdR(DBD) 137 and an sfGFP reporter

Depositor

Jeffrey Tabor

Article

Cartwright et al Proc Natl Acad Sci U S A. 2021 May 18;11

Inserts

SO_4388(REC)-PsdR(DBD) 137
sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23108 and PpsdA110

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pWS5397-CRISPRai-NatR

Plasmid

#182836

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - NatR marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG415GPD-ccdB

Plasmid

#14146

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-peroxi

Plasmid

#137806

Purpose

Visualization of peroxisomes

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv 160374

Insert

SKL

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-mCherry

Plasmid

#75161

Purpose

Derived from LentiCRISPR v1 but expresses mCherry instead of puromycin resistance. 3rd generation.

Depositor

Beat Bornhauser

Article

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Abl-His6-FLAG

Plasmid

#52684

Purpose

expression of Abl in mammalian cells

Depositor

Benjamin Turk

Article

Deng et al Chembiochem. 2012 Nov 26;13(17):2510-2.

Insert

ABL1 (Abl1 Mouse)

Use

Tags

FLAG and His6

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pSR357.24

Plasmid

#125109

Purpose

Expression of narL(1-159aa)-ydfI(153-213aa) chimera under PLtetO-1, output Promoter PydfJ115

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

narL(REC)-ydfI(DBD)159
sfgfp
tetR

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-VEGFR2-780

Plasmid

#21307

Depositor

Donald Ingber

Article

Mammoto et al Nature. 2009 Feb 26. 457(7233):1103-8.

Insert

VEGFR2 promoter (KDR Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

May 29, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS410

Plasmid

#11258

Depositor

Fred Cross

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 24, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-VHL wt-pBabe-puro

Plasmid

#19234

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

wt

Promoter

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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vYM112_PB-pEF1a-optiPIN2-HA(Hygro)

Plasmid

#160045

Purpose

For expressing the active auxin pump PIN2

Depositor

Michael Elowitz

Article

Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

Insert

PIN2

Use

Synthetic Biology

Tags

HA

Expression

Mammalian

Mutation

codon optimized

Promoter

pEF1s

Available since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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