GFP-Lamin-A
(Plasmid #206027)

• Purpose: Expresses GFP-tagged Lamin A in mammalian cells
• Depositing Lab: Geert van den Bogaart
• Publication: Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi: 10.1016/j.celrep.2024.113866.

Backbone

• Vector backbone: peGFP-C1
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Lamin-A
• Alt name: LMNA
• Alt name: LMN1
• Species: H. sapiens (human)
• Insert Size (bp): -42
• GenBank ID: NM_001257374.2
• Entrez Gene: LMNA (a.k.a. CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1)
• Promoter: CMV
• Tag / Fusion Protein:
  • eGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CATGGTCCTGCTGGAGTTCGTG
• 3′ sequencing primer: GAAATTTGTGATGCTATTGC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Synthetic gene by Genscript

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry