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Plasmid#20236DepositorInsertdKDM4A (Kdm4A Fly)
UseTagsFLAG, His, and TEVExpressionInsectMutationchanged His 195 to AlaPromoterAvailable sinceMarch 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFastBacHT (His10-4xFRB-SNAP-Lck G2A)
Plasmid#177887PurposeBaculo expression of decahistidine tag fused to 4xFRB, 1xSNAP tag and human Lck G2ADepositorInsertLck (LCK Human)
UseTags10xHis-TEV-4xFRB-SNAP tagExpressionInsectMutationG2APromoterPolyhedrinAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pFastBac(His10-Lckg2a,Y394F,Y505F)
Plasmid#177894PurposeBaculo expression of His10 tag fused to human LckG2ADepositorInsertLck (LCK Human)
UseTags10xHis-TEVExpressionInsectMutationG2A,Y394F,Y505FPromoterPolyhedrinAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-33
Plasmid#214342PurposeProtein overexpression in bacteriaDepositorInsertIL33 (IL33 Human)
UseTagsHis-TEV and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXWJ109As(RS-te-AAV)
Plasmid#123156PurposeFor TEV-based leakiness regulation. pSB3K3 carrying J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-te-AAV-tDepositorInsertJ109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-te-AAV-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ109, ParsR, PhrpLEAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cas12aUltra_5XNLS
Plasmid#218775PurposeHis-TEV-Cas12aUltra-BPSV40_cMyc_NP_5NLSDepositorInsert5xNLS-BPSV40-NLP-cMyc-cMyc-BPSV40- AspCas12a
UseCRISPRTags6XHis (C terminal), HA, Nucleoplasmin, SV40, and …ExpressionBacterialMutationNucleotides mutations were made to encode for arg…PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-1β
Plasmid#214340PurposeProtein overexpression in bacteriaDepositorInsertIL1B (IL1B Human)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF5B-FRT-GFP-αTAT1
Plasmid#27099DepositorInsertα-Tubulin K40 acetyltransferase1 (ATAT1 Human)
UseTagsGFP, S-tag, and TEV siteExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_ERBB2-V5
Plasmid#214612PurposeERBB2 receptor for ERBB2/ERBB3 heterodimer split TEV recuitment assayDepositorInsertERBB2 (ERBB2 Human)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FlipCherry-T2A-GFP
Plasmid#124436PurposeExpresses FlipCherry (TEV cleavage sequence) and T2A GFP in mammalian cellsDepositorInsertFlipCherry-T2A-GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
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Fyn-SH3_5WT_S25C
Plasmid#118824PurposeBacterial expression of the SH3 domain of human FynDepositorInsertFYN (FYN Human)
UseTags6-His-TEV tagExpressionBacterialMutationS25C; See depositor comments below.PromoterAvailable sinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBiex PKC - Peptide 8 GFP RFP
Plasmid#83564PurposeExpresses a GFP-RFP FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.DepositorInsertPKC alpha (PRKCA Human)
UseTags10 nm ER/K Helix, FLAG purification tag, TEV Prot…ExpressionBacterial and InsectMutationOnly catalytic domain of PKC (aa 335-672)PromoterT7 PromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-36γ
Plasmid#214345PurposeProtein overexpression in bacteriaDepositorInsertIL36G (IL36G Human)
UseTagsHis-TEV and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJSC114 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant
Plasmid#101215PurposeBacterial expression plasmid for SpCas9 eSpCas9(1.1) variantDepositorInsertSpCas9 variant K848A/K1003A/R1060A
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationK848A, K1003A and R1060APromoterT7Available sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
Wild-type CoV-2 ORF8
Plasmid#159933PurposeProtein overexpression in E.coliDepositorInsertCoV-2 ORF8 18-121 (ORF8 CoV-2)
UseTags6x Histidine, TEV cleavableExpressionBacterialMutationNonePromoterT7Available sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
3NES-CS(G)-tTA-CS(G)-3NES
Plasmid#137836PurposeSoluble tTA transcription factor flanked by N and C terminal TEV protease G cleavage sequences and 3 nuclear export sequencesDepositorInsert3NES-CD(G)-tTA-CS(G)-3NES
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-1α
Plasmid#214341PurposeProtein overexpression in bacteriaDepositorInsertIL1A (IL1A Human)
UseTagsHis-TEV and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-36β
Plasmid#214344PurposeProtein overexpression in bacteriaDepositorInsertIL36B (IL36B Human)
UseTagsHis-TEV and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-GST-EGFP
Plasmid#177136PurposeBacterial vector for expression of an N-terminal GST fusion of EGFP with a TEV protease site located between the GST tag and EGFPDepositorInsertEGFP
UseTagsExpressionBacterialMutationPromoterTacAvailable sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only