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Plasmid#197278PurposeEncodes for viral expression of Synaptotagmin 1 (SYT1) for use in the RUSH system; visualized with HaloTag. Reporter only (no hook). SYT1 is mutated to disrupt palmitoylation and glycosylation.DepositorInsertSYT1 and HaloTag (Syt1 Rat)
UseLentiviralTagsFLAGExpressionMutationT15A, T16A, N24Q, C74A, C75A, C77A, C79A, and C82APromoterhSyn (human synapsin I promoter)Available sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJR017
Plasmid#198835PurposeIn vivo blocking of synaptic signaling through Tetanus toxin mediated cleavage of synptobrevin proteins.DepositorInsert15xUAS::TeTX-SL2-mKate::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-SVA-lncRNA AK057321 shRNA 1S
Plasmid#202836PurposeLentiviral vector that expresses an shRNA that knocks down SVA-lncRNA AK057321 expressionDepositorInsertSVA-lncRNA AK057321 shRNA 1S
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-SVA-lncRNA AK057321 shRNA 2S
Plasmid#202838PurposeLentiviral vector that expresses an shRNA that knocks down SVA-lncRNA AK057321 expressionDepositorInsertpLVX-SVA-lncRNA shRNA 2S
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-SVA-lncRNA-AK057321 shRNA 1A
Plasmid#202834PurposeLentiviral vector that expresses an shRNA that knocks down SVA-lncRNA AK057321 expressionDepositorInsertSVA-lncRNA AK057321 shRNA 1A
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-SVA-lncRNA-AK057321-mcherry
Plasmid#202813PurposeLentiviral vector to express of SVA-lncRNA AK057321 with CMV for mcherry expressionDepositorInsertSVA-lncRNA AK057321
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBa.Halo-KIF3B tail 391-747
Plasmid#193929PurposeMammalian expression of Halo-tagged Kif 3B tail 391-747DepositorInsertKif 3B (Kif3b Mouse)
UseTagsHaloExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJR032
Plasmid#198773PurposeStabilized step function Opsins ChR2 variantDepositorInsert15xUAS::hChR2 (C128S, D156A)-EYFP::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJR035
Plasmid#198821PurposeFor tissue specific RNA-seqDepositorInsert15xUAS::rpl-22HA-SL2-mKate::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHW491
Plasmid#198808PurposeIn vivo blocking of synaptic signaling through Tetanus toxin mediated cleavage of synptobrevin proteins.DepositorInsert15xUAS::Tetx cDNA::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJL049
Plasmid#198809PurposeIn vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity.DepositorInsert15xUAS::GCaMP6s-SL2-mKate2::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJR031
Plasmid#198825PurposeStable step function ChR2 variant. Activated by bluelight, terminated by green light.DepositorInsert15xUAS::ChR2(C128S)-EYFP::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJR021
Plasmid#198814PurposeVariant of yellow fluorescent protein for transient gene expressionDepositorInsert15xUAS::destabilizedYFP::let-838 3'UTR
UseTagsExpressionWormMutationYFP with destabilized signal from mouse ornithine…PromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJR034
Plasmid#198829PurposeStep-Waveform Inhibitory ChannelrhodopsinDepositorInsert15xUAS::SwiCHR-TS-EYFP::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJR033
Plasmid#198822PurposeChloride-conducting channelrhodopsinDepositorInsert15xUAS::UAS-iC1-c2-TS-EYFP::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 gfaABC1D-HA-BioID2-SAPAP3
Plasmid#176858PurposeExpresses SAPAP3 protein fused with BioID2 at the N-terminus in astrocytesDepositorInsertHA-BioID2-SAPAP3
UseAAVTagsHAExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_CaMKIIa_SERT_mCherry
Plasmid#198736PurposeSerotonin transporter with mCherry fluorescent protein driven by CaMKIIa promoter on a AAV vectorDepositorInsertSerotonin transporter and mCherry
UseAAVTagsExpressionMammalianMutationPromoterCaMKIIaAvailable sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_CaMKIIa_SERT_EYFP
Plasmid#198737PurposeSerotonin transporter with EYFP fluorescent protein driven by CaMKIIa promoter on a AAV vectorDepositorInsertSerotonin transporter and yellow fluorescent protein
UseAAVTagsExpressionMammalianMutationPromoterCaMKIIaAvailable sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
DsRed -7e
Plasmid#191765PurposeExpression of DsRed fused to a negatively charged polypeptide. Overall charge on tetramer: -7eDepositorInsertDsRed
UseTagsN terminal fusion of negatively charged polypetod…ExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-SuperNovaGreen-T2A-dTomato
Plasmid#193798PurposeExpresses SuperNova Green in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomatoDepositorInsertSuperNova Green
UseAAVTagsFlag tagExpressionMammalianMutationPromoterCAGAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF3A tail 419-702
Plasmid#193923PurposeMammalian expression of GFP-tagged Kif 3A tail 419-702DepositorInsertKif 3A (KIF3A Human)
UseTagsGFPExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Tet-On shYAP1-6
Plasmid#193667PurposeTet inducible knockdown of YAP1DepositorInsertYAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Tet-On shYAP1-2
Plasmid#193665PurposeTet inducible knockdown of YAP1DepositorInsertYAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
PL452-FGF8-LA-p2a-tdTomato-hGHpA-PGK-neoR-FGF8-RA
Plasmid#191687PurposeFor knocking p2a-tdTomato into FGF8 locusDepositorInsertFGF8 homology arms and p2a-tdTomato
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
PL452-PTPRO-LA-p2a-EGFP-hGHpA-PGK-neoR-PTPRO-RA
Plasmid#191689PurposeFor knocking p2a-EGFP into PTPRO locusDepositorInsertPTPRO homology arms and p2a-EGFP
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-GFP ArgSen delta Degradation Domain
Plasmid#184701PurposeLentiviral transfer plasmid for expression of GFP ArgSen delta Degradation DomainDepositorInsertGFP ArgSen delta Degradation Domain
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-GFP ArgSen delta Disordered Region
Plasmid#184702PurposeLentiviral transfer plasmid for expression of GFP ArgSen delta Disordered RegionDepositorInsertGFP ArgSen delta Disordered Region
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
dora[B]_rescue_construct
Plasmid#190637PurposePuromycin-selectable expression of Dora[T295M] (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
UseTagsExpressionInsectMutationChanged threonine 295 to methioninePromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-dora[B]_rescue_construct
Plasmid#190638PurposePuromycin-selectable expression of HA-tagged Dora[T295M] (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
UseTags3xHAExpressionInsectMutationChanged threonine 295 to methioninePromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
dora[A]_rescue_construct
Plasmid#190639PurposePuromycin-selectable expression of truncated Dora (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
UseTagsExpressionInsectMutationTruncated to contain amino acids 1-945PromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-dora[A]_rescue_construct
Plasmid#190640PurposePuromycin-selectable expression of HA-tagged truncated Dora (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
UseTags3xHAExpressionInsectMutationTruncated to contain amino acids 1-945PromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-L1-Kozak-Ark-L2
Plasmid#186362PurposeEntry clone with ORF encoding Ciona robusta Aurora kinase A flanked by Gateway recombination sequences. No Stop sequence: to be recombined into a destination vector with a C-terminal protein tag.DepositorInsertCiona robusta Aurora kinase A
UseExpression of a fluorescent centrosome markerTagsExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
attB53-Pac-tetO-mCherry-attB53
Plasmid#183612PurposeRMCE vector to shuttle puromycin resistance and tetO-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).DepositorInserttetO-mCherry
UseRmceTagsExpressionMutationPromotertetOAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-ZNF267
Plasmid#178452Purposedoxycycline-inducible expression of Human ZNF267 in mammalian cellsDepositorInsertZNF267 (ZNF267 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-PLEK
Plasmid#178439Purposedoxycycline-inducible expression of Human PLEK in mammalian cellsDepositorInsertPLEK (PLEK Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH3453
Plasmid#179577PurposePglc-55B::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans AVB/others premotor IN and others expression of miniSOG-SL2 BFPDepositorInserttomm20::miniSOG (tomm-20 Nematode)
UseTagsBFPExpressionWormMutationPromoterPlgc-55BAvailable sinceMarch 1, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJH2890
Plasmid#179561PurposePlgc-55B::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans AVB premotor IN and other neurals expression of miniSOG-SL2 RFPDepositorInserttomm20::miniSOG (tomm-20 Nematode)
UseTagswCherryExpressionWormMutationPromoterPlgc-55BAvailable sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
MO70-C-FLAG-hNKD2
Plasmid#174327Purposeencodes human NKD2 cDNA with a C-terminal FLAG tag in retroviral expression vector. Encodes GFP from IRES site and puromycin resistanceDepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
UseRetroviralTagsFLAG TagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pClgn1.1_Kctd19-3xHA
Plasmid#173497PurposeExpress Kctd19-3xHA under the mouse Clgn promoter.DepositorInsertPotassium Channel Tetramerization Domain Containing 19 (Kctd19 Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFendoNA2S
Plasmid#174730PurposeExpresses GFP-endoNA2-S706A fusion protein for detection of polysialic acid. The endoNA2-S706A is a noncatalytic endosialidase that lacks the ability for releasing its C-terminal chaperone domain.DepositorInsertendoNA2-S706A
UseTagsExpressionBacterialMutationChanged Serine 706 to AlaninePromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pClgn1.1_3xFLAG-Kctd19
Plasmid#173498PurposeExpress 3xFLAG-Kctd19 under the mouse Clgn promoter.DepositorInsertPotassium Channel Tetramerization Domain Containing 19 (Kctd19 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIRES-EGFP-TPD53
Plasmid#172461PurposeExpression of untagged Tumor Protein D52 like 1 (TPD53/TPD52L1) and GFPDepositorInsertTPD53 (TPD52L1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIRES-EGFP-TPD54
Plasmid#172459PurposeExpression of untagged Tumor Protein D52 like 2 (TPD54/TPD52L2) and GFPDepositorInsertTPD54 (TPD52L2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-3)
Plasmid#159664PurposeCRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.DepositorInserthSpCas9
UseCRISPRTagsExpressionMutationPromoterEf1aAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMiniT:myc-GluA2 TKIT donor
Plasmid#169423PurposeDonor DNA for TKIT knockin of Myc to GluA2 in mouseDepositorInsertMyc (Myc Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_shRNA resistant PKA-Cα(E12,14K)-mEGFP
Plasmid#168491PurposeMammalian expression of the E12,14K mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.DepositorInsertthe E12,14K mutant of the mouse PKA catalytic subunit α C-terminally tagged by mEGFP. Resistant to shRNA knock-down.
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTEF1-UDH
Plasmid#166679PurposeYeast integrative plasmid for expressing P. syringae uronate dehydrogenase, with TEF1 promoter. Contains leucine auxotrophic marker (K. lactis LEU2).DepositorInsertUDH
UseSynthetic BiologyTagsExpressionYeastMutationPromoterTEF1Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-2)
Plasmid#159663PurposeCRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.DepositorInserthSpCas9
UseCRISPRTagsExpressionMutationPromoterEf1aAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-1)
Plasmid#159662PurposeCRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.DepositorInserthSpCas9
UseCRISPRTagsExpressionMutationPromoterEf1aAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-1)
Plasmid#159656PurposeCRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.DepositorInserthSpCas9
UseCRISPRTagsExpressionMutationPromoterEf1aAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only