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Plasmid#73288PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-5DepositorInsertguiding RNA
UseCRISPR; GrnaTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB3044(gRNA XI-2)
Plasmid#73286PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-2DepositorInsertguiding RNA
UseCRISPR; GrnaTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB3048(gRNA XII-2)
Plasmid#73290PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-2DepositorInsertguiding RNA
UseCRISPR; GrnaTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG/hArf4(WT)-EGFP
Plasmid#79407PurposeExpresses C-terminally EGFP-tagged Arf4(WT) in mammalian cellsDepositorInsertArf4 (ARF4 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-FLAG/MKRN1(1-369)
Plasmid#78753PurposeTo overexpress MKRN1(1-369) in Mammalian CellsDepositorInsertMKRN1 (MKRN1 Human)
UseTagsFLAGExpressionMammalianMutation1-369PromoterCMVAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB3043(gRNA XI-1)
Plasmid#73285PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-1DepositorInsertguiding RNA
UseCRISPR; GrnaTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB3049(gRNA XII-4)
Plasmid#73291PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-4DepositorInsertguiding RNA
UseCRISPR; GrnaTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pSG5-FLAG-CaMKKbeta rat S129D, S133D, S137D
Plasmid#32457DepositorInsertCalcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)
UseTagsFLAGExpressionMammalianMutationS129D, S133D, S137D (S3V, G96R, and P108L also pr…PromoterSV40Available sinceApril 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
Lentiviral FLAG-CaMKKbeta S129D, S133D, S137D
Plasmid#32460DepositorInsertCalcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)
UseLentiviralTagsFLAGExpressionMutationS129D, S133D, S137DPromoterhuman PGKAvailable sinceFeb. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-CaMKKbeta S129A, S133A, S137A
Plasmid#32454DepositorInsertCalcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)
UseTagsFLAG and GFPExpressionMammalianMutationS129A, S133A, S137APromoterCMVAvailable sinceApril 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCA24N-ligase-pprA
Plasmid#87744PurposeIPTG-inducible expression of ligase-pprA fusion for protein purificationDepositorInsertT4 DNA ligase (30 )
UseTags6xHis and codon optimized pprA (from Deinococcus …ExpressionBacterialMutationPromoterT5-lacAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK-flag-USP19 494-1216 (USP)
Plasmid#78588PurposeExpression flag-USP19 494-1216 (USP) in mammalian cellsDepositorInsertflag-USP19 494-1216 (USP19 Human)
UseTagsflagExpressionMammalianMutationdeleted amino acid 1-493 and mutated 1216 residue…PromoterCMVAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSG5-FLAG-CaMKKbeta rat S100A, S495A and S511A
Plasmid#33320DepositorInsertCalcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)
UseTagsFLAGExpressionMammalianMutationS99A, S494A, S510APromoterSV40Available sinceApril 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
GFP-DroshaS300A
Plasmid#62526PurposeSerine 300 of Drosha is mutated to alanineDepositorInserthuman Drosha with serine 300 changed to alanine (DROSHA Human)
UseTagsGFPExpressionMammalianMutationchanged serine 300 to alaninePromoterAvailable sinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-DroshaS302A
Plasmid#62527PurposeSerine 302 of Drosha is mutated to alanineDepositorInserthuman Drosha with serine 302 changed to alanine (DROSHA Human)
UseTagsGFPExpressionMammalianMutationchanged serine 302 to alaninePromoterAvailable sinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNAS1b split mCherry NEDD4 WW2
Plasmid#111882PurposeMode #2 phosphosite expression (split mCherry, using NEDD4 WW2 binding domain)DepositorTypeEmpty backboneUseTagsSplit mCherry system: NEDD4 WW2 fused to C-termin…ExpressionBacterialMutationPromoterAvailable sinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG/MKRN1(1-280)
Plasmid#78752PurposeTo overexpress MKRN1(1-280) in Mammalian CellsDepositorInsertMKRN1 (MKRN1 Human)
UseTagsFLAGExpressionMammalianMutation1-280PromoterCMVAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG/MKRN1(110-482)
Plasmid#78754PurposeTo overexpress MKRN1(110-482) in Mammalian CellsDepositorInsertMKRN1 (MKRN1 Human)
UseTagsFLAGExpressionMammalianMutation110-482PromoterCMVAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAD02_CBASS_Methylosarcina_fibrata
Plasmid#224406PurposeFor expression of the type I CBASS system encoded by Methylosarcina fibrataDepositorInsert4TM_MfCdnH_AGS-C
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD02_CBASS_Vibrio_vulnificus
Plasmid#224405PurposeFor expression of the type I CBASS system encoded by Vibrio vulnificusDepositorInsertEndonuclease_Phospholipase_VvCdnG_AGS-C
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD02_CBASS_Escherichia_coli_CVM_N37067PS
Plasmid#224403PurposeFor expression of the type I CBASS system encoded by Escherichia coli CVM N37067PSDepositorInsertEcCdnF_AGS-C_SLATT6
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD02_CBASS_Pseudoalteromonas_sp._A2
Plasmid#224401PurposeFor expression of the type I CBASS system encoded by Pseudoalteromonas sp. A2DepositorInsertPsp.CdnB_AGS-C_Cap15
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD02_CBASS_Alteromonas_sp._ALT199
Plasmid#224399PurposeFor expression of the type I CBASS system encoded by Alteromonas sp. ALT199DepositorInsertAsp.CdnB_AGS-C_Cap15
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD02_CBASS_Escherichia_coli_K71-77
Plasmid#224398PurposeFor expression of the type I CBASS system encoded by Escherichia coli K71-77DepositorInsertPhospholipase_EcCdnE_DisA-like
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD02_CBASS_Escherichia_coli_RU1_BHI6
Plasmid#224397PurposeFor expression of the type I CBASS system encoded by Escherichia coli RU1 BHI6DepositorInsertCap15_EcCdnE
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD02_CBASS_Xanthomonas_phaseoli
Plasmid#224392PurposeFor expression of the type I CBASS system encoded by Xanthomonas phaseoliDepositorInsertXpCdnC_Endonuclease
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28Man
Plasmid#203399Purposeexo-beta mannosidase useful in enzymatic biotransformations of glycans and in coupled assays of various carbohydrate processing enzymesDepositorInsertman2A
UseTagsH6ExpressionBacterialMutationNonePromoterT7Available sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-IFT57
Plasmid#218728PurposeExpresses N-terminally EGFP-tagged IFT57 in mammalian cellsDepositorInsertIFT57 (IFT57 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
RepairTemplate_NIPBL_mEGFP_mAID
Plasmid#217664PurposeRepair template to endogenously tag NIPBL with mEGFP-mAID TagDepositorInsertNIPBL_mEGFP_mAID (NIPBL Human)
UseTagsmAID and meGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF1b-His8-TEV-PI31-1-151
Plasmid#202593PurposeBacterial expression of HsPI31-1-151DepositorInsertPI31 (PSMF1 Human)
UseTags8xHis, TEVExpressionBacterialMutationaa 1-151 onlyPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Cas9-hGem_gRNA2
Plasmid#217659Purposeexpresses Cas9-hGem and guideRNA targeting the human AAVS1 safe harbour locusDepositorInsertsgRNA2 targeting the human AAVS1 safe harbour locus
UseCRISPRTagsExpressionBacterial and MammalianMutationPromoterU6Available sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCALPS-Flag-Miwi-EGFP
Plasmid#212575PurposeLentiviral expression of Flag-tagged mouse Miwi in mammalian cellsDepositorInsertMiwi (Piwil1 Mouse)
UseLentiviralTags5x Flag and SNAP tagExpressionMammalianMutationPromoterSFFVAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF702
Plasmid#209650PurposeSuicide plasmid carrying a neomycin resistance marker flanked by homologous regions of the Mth60-fimbria encoding operon of Methanothermobacter thermautotrophicusDepositorInsertsDownstream homologous region
thermostable neomycin phosphotransferase gene
Upstream homologous region
UseTagsExpressionMutationPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
hPRL2_1-163
Plasmid#209794PurposeBacterial expression of human PRL-2 without farnesylation motifDepositorInsertPRL-2 (PTP4A2 Human)
UseTags6xHisExpressionBacterialMutationaa 1-163 onlyPromoterT7Available sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
FKBP12-CB2mutx2-mTagBFP2-HAx2
Plasmid#207147PurposeTandem mutant calcium-buffer with rapalog dimerizerDepositorInsertCalbindin-2 (CALB2 Human)
UseTagsFKBP12, HA, and mTagBFP2ExpressionMammalianMutationE40Q, E87Q, E131Q, E175Q, E219QPromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pROF018
Plasmid#155325PurposeConstitutive expression of KRAB-EL222 in plantsDepositorInsertPCaMV35S-NLS-KRAB-EL222-Tnos
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pROF050
Plasmid#155327PurposeConstitutive expression of BRD-EL222 in plantsDepositorInsertPCaMV35S-BRD-NLS-EL222-Tnos
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-RnCENP-O-GFP
Plasmid#205179PurposeExpresses rat CENP-O C-term tagged with GFP under T7 promoter - designed for in vitro transcriptionDepositorInsertCENP-O (Cenpo Rat)
UseIn vitro transcriptionTags5 glycines linker followed by GFPExpressionMammalianMutationWild type rat CENP-OPromoterT7Available sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSynapsin-Nluc-EPAC-VV
Plasmid#204846PurposeProduce lentivirus encoding Nluc-EPAC sensor in neuronal cell typeDepositorInsertNluc-EPAC sensor
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPAN2-G132EL133EP134RT137R-dsRNAres_Y
Plasmid#148109PurposeInsect Expression of DmPAN2-G132EL133E134RT137R-dsRNAresDepositorInsertDmPAN2-G132EL133E134RT137R-dsRNAres (PAN2 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPAN2-del975-1031GSSG-dsRNAres_Y
Plasmid#148069PurposeInsect Expression of DmPAN2-del975-1031GSSG-dsRNAresDepositorInsertDmPAN2-del975-1031GSSG-dsRNAres (PAN2 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPAN2-F99E-dsRNAres_Y
Plasmid#148072PurposeInsect Expression of DmPAN2-F99E-dsRNAresDepositorInsertDmPAN2-F99E-dsRNAres (PAN2 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPAN2-F99EG132EL133E-dsRNAres_Y
Plasmid#148073PurposeInsect Expression of DmPAN2-F99EG132EL133E-dsRNAresDepositorInsertDmPAN2-F99EG132EL133E-dsRNAres (PAN2 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPAN2-F550EM554EK557E-dsRNAres_Y
Plasmid#148074PurposeInsect Expression of DmPAN2-F550EM554EK557E-dsRNAresDepositorInsertDmPAN2-F550EM554EK557E-dsRNAres (PAN2 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPAN2-G132EL133E-dsRNAres_Y
Plasmid#148076PurposeInsect Expression of DmPAN2-G132EL133E-dsRNAresDepositorInsertDmPAN2-G132EL133E-dsRNAres (PAN2 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPAN2-K1124EY1127EK1131E-dsRNAres_Y
Plasmid#148077PurposeInsect Expression of DmPAN2-K1124EY1127EK1131E-dsRNAresDepositorInsertDmPAN2-K1124EY1127EK1131E-dsRNAres (PAN2 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPAN2-R245E-dsRNAres_Y
Plasmid#148078PurposeInsect Expression of DmPAN2-R345E-dsRNAresDepositorInsertDmPAN2-R345E-dsRNAres (PAN2 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPAN2-T97RH102G-dsRNAres_Y
Plasmid#148079PurposeInsect Expression of DmPAN2-T97RH102G-dsRNAresDepositorInsertDmPAN2-T97RH102G-dsRNAres (PAN2 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only