hPRL2_1-163
(Plasmid #209794)

• Purpose: Bacterial expression of human PRL-2 without farnesylation motif
• Depositing Lab: Kalle Gehring
• Publication: Kozlov et al J Biol Chem. 2020 Aug 14;295(33):11682-11692. doi: 10.1074/jbc.RA120.014464. Epub 2020 Jun 22.

Backbone

• Vector backbone: pET15b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5708
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PRL-2
• Species: H. sapiens (human)
• Mutation: aa 1-163 only
• Entrez Gene: PTP4A2 (a.k.a. HH13, HH7-2, HU-PP-1, OV-1, PRL-2, PRL2, PTP4A, PTPCAAX2, ptp-IV1a, ptp-IV1b)
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7 Fwd
• 3′ sequencing primer: T7 Rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: codon-optimized gene synthesis

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry