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Plasmid#127831PurposeProduction of recombinant human ribosomal protein L11DepositorInsertRPL11 (RPL11 Human)
UseTagsHis6 - Thioredoxin - TEVExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP-Leu2d_GgPCFT
Plasmid#165414Purposeexpression GgPCFT protein with C terminally tagged GFP-His tag cleavable with TEV protease.DepositorInsertGgPCFT (SLC46A1 Chicken, Synthetic)
UseTagsGFP-His8ExpressionYeastMutationPromoterGAL1Available sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNH-TrxT-G-S18
Plasmid#127827PurposeProduction of recombinant human ribosomal protein S18DepositorInsertRPS18 (RPS18 Human)
UseTagsHis6 - Thioredoxin - TEVExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cwc25-pRSETA
Plasmid#61043PurposeEncodes the yeast Cwc25 protein for overexpression and purification using a C-terminal hexahistidine tag. A single cysteine residue is encoded after the Cwc25 sequence for fluorescent labeling.DepositorInsertCwc25
UseTagsTEV site, Histidine TagExpressionBacterialMutationPromoterT7Available sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pET24a_SMOX
Plasmid#186345PurposeC-terminal His-Tag and a TEV cleaveage site, to transform into E. coli BL21 (DE3) cellsDepositorInsertSpermine oxidase (SMOX Human)
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-15b-aδNb072
Plasmid#204627PurposeExpresses the anti-IgD nanobody 072 (aδNb072) in the bacterial periplasm. aδNb072 is an orthosteric inhibitor of human anti-Phl p 7 antibodies of clone 102.1F10.DepositorInsertaδNb072
UseTagsHis6 and TEV protease cleavage siteExpressionBacterialMutationPromoterT7Available sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPI528
Plasmid#207442Purpose6xHis-SS-TEV-ErkB-T176A-Y178A bacterial expression vector - catalytically inactiveDepositorInsertErkB
UseUnspecifiedTagsExpressionMutationT176A, Y178APromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Halo-S13S2DBD
Plasmid#200196PurposeExpression of Sox13 with Sox2 DBDDepositorUseTagsHaloExpressionMammalianMutationSox13(1-1242); Sox 2 (126-333); Sox13 (1449-1839)PromoterEF_1alphaAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
NLS-Halo-HSF2
Plasmid#200198PurposeExpression of full length HSF2 with SV40 NLSDepositorInsert3X SV40 NLS-Halo-HSF2 (Hsf2 Mouse)
UseTagsHaloExpressionMammalianMutationNonePromoterEF_1alphaAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
Halo-H2DBD-HR
Plasmid#200199PurposeExpression of truncated HSF2 (DBD and HR)DepositorInsertHalo-H2DBD-HR (Hsf2 Mouse)
UseTagsHaloExpressionMammalianMutationHSF2 (1-627)PromoterEF_1alphaAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
Halo-H2DBD
Plasmid#200200PurposeExpression of truncated HSF2 (DBD)DepositorInsertHalo-H2DBD (Hsf2 Mouse)
UseTagsHaloExpressionMammalianMutationHSF2 (1-366; 15 bp linker; 972-1017)PromoterEF_1alphaAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-Halo-HSF2
Plasmid#200202PurposeExpression of full length HSF2DepositorInsertHA-Halo-HSF2 (Hsf2 Mouse)
UseTagsHaloExpressionMammalianMutationNonePromoterEF_1alphaAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGST-parallell2-GGA3(571-723)
Plasmid#199150PurposeExpresses GST-GGA3(571-723) in bacteriaDepositorInsertGGA3(571-723) (GGA3 Human)
UseTagsGST and TEV protease cleavage siteExpressionBacterialMutationPromotertacAvailable sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET29a(+)::D1141A
Plasmid#192963PurposeTest the activity of the putative aspartyl protease motif DPXGL-(18)-DPXGLDepositorInsertTse5_D1141A
UseTags9xhis + TEV tagExpressionBacterialMutationD1141APromoterAvailable sinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET29a(+)::9xhis-Tse5
Plasmid#192962PurposeHeterologous expression of Tse5 in E. coli Lemo21 cells for purification of Tse5-CTDepositorInsertTse5
UseTags9xhis + TEV tagExpressionBacterialMutationPromoterAvailable sinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
FnRPR9-10
Plasmid#182568PurposeHuman fibronectin fragment, type-III repeats 9-10, R1374A, P1376A, R1379A mutantDepositorInsertHuman fibronectin fragment, type-III repeats 9-10
UseTagsHis and TEV cleavage site after His tagExpressionBacterialMutationR1374A, P1376A, R1379APromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
FnRPR8-10
Plasmid#182570PurposeHuman fibronectin fragment, type-III repeats 8-10, R1374A, P1376A, R1379A mutantDepositorInsertHuman fibronectin fragment, type-III repeats 8-10
UseTagsHis and TEV cleavage site after His tagExpressionBacterialMutationR1374A, P1376A, R1379APromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
VAMP8
Plasmid#186689PurposeExpresses VAMP8 in E. ColiDepositorInsertVAMP8
UseTags3xFLAG, GST, TEV, and ThrombinExpressionBacterialMutationPromoterT7Available sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2196
Plasmid#170400PurposeFor expresson of LetA-[LetB PLL6->1] operon from E. coli. N-terminal his tag on LetA, araBAD promoterDepositorUseTags8xHis2xQH and TEV cleavage site on LetAExpressionBacterialMutationLetA-LetB(Δ702-717, replaced with 114-130)PromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only