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Plasmid#39280DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutation-1163 to +6 of nmt1 locusPromoterAvailable sinceAug. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
HO-pGAL-poly-KanMX4-HO
Plasmid#51664Purposeyeast plasmid for integration of target sequence at HO locus, Kan selection, pGAL inducible promoterDepositorTypeEmpty backboneUseYeast integrationTagsExpressionYeastMutationPromoterAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-Purg1
Plasmid#39349DepositorUseYeast genomic targetingTagskanMX6 module - reverse orientationExpressionMutationPromoterAvailable sinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKT-iRFP-KAN
Plasmid#64687PurposepKT vector encoding yeast-codon optimized infrared fluorescent protein iRFP.DepositorInsertiRFP
UseTagsExpressionYeastMutationNucleotide sequence has been yeast-codon optimize…PromoterAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pREF-GFP kan
Plasmid#122061Purposereporter plasmid for termination and terminator override to express GFP. Marked with URA3DepositorInsertsintergenic terminator from yeast IMD2
green fluorescent protein
UseTagsExpressionYeastMutationPromoterGAL1 and GAL1 comes in pYES2Available sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mScarletI-KanMX6
Plasmid#136690PurposeTagging genes in Cterm with mScarletI in fission yeastDepositorInsertmScarletI
UseTagsmScarletIExpressionYeastMutationPromoterNoneAvailable sinceJune 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P41nmt1
Plasmid#39281DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutation-1163 to +6 of nmt1 locus with a 4bp deletion (TA…PromoterAvailable sinceSept. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P81nmt1
Plasmid#39282DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutation-1163 to +6 of nmt1 locus with a 7bp deletion (AT…PromoterAvailable sinceSept. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSUM-Kan-MCS2
Plasmid#109289PurposeExpresses high levels of protein in mycobacteriaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationpSUM-kan-MCS1-gfp was modified to reorient the Ba…PromoterAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-yibDp-GFPuv
Plasmid#127078PurposeLow phosphate inducible yibD gene promoter expressing GFPuv reporterDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromoteryibDpAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-HBH-kanMX6
Plasmid#26872DepositorInsertHBH tag
UsePcr tagging yeastTagsExpressionMutationPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKan cpRBP-FL P53
Plasmid#174504PurposeExpress Full-length P53 in E.coli with cleavable expression/purification tag (ribose binding protein circular permutant)DepositorInsertP53 gene (TP53 Human)
UseTags20 amino acid linker containing HRV3C protease si…ExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a- mCFP-kanMX6
Plasmid#105145PurposeYeast gene targetingDepositorInsertmCFP-kanMX6
UseTagsmCFPExpressionBacterial and YeastMutationA206KPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDZ617 pKAN 12xPP7 V4
Plasmid#72237PurposeFloxed Kanamycin (G418) selection maker; 3' tagging vector used to add 12 repetitive PP7 version 4 RNA stem-loops to gene of interestDepositorInsert12xPP7 V4
UseCre/LoxTagsExpressionBacterialMutationPromoterAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mEOS3.2-KanMX6
Plasmid#87030PurposeTag S. pombe genes in C terminal with mEOS3.2DepositorInsertmEOS3.2
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mMaple3-kanMX6
Plasmid#105165PurposeYeast gene targetingDepositorInsertmMaple3-kanMX6
UseTagsGlySerGly-mMaple3ExpressionBacterial and YeastMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-yibDp2-GFPuv
Plasmid#139083PurposeLow phosphate inducible promoter yibDp2 for GFP expressionDepositorInsertyibDp2-GFPuv
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-URA3-KanMX6
Plasmid#53194Purposegene deletion with URA3-kanMX6 double markerDepositorInsertURA3-KanMX6
UseYeast genomic targetingTagsExpressionMutationPromoterAvailable sinceMay 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-PA-GFP-kanMX6
Plasmid#18820DepositorInsertPhotoactivatable Green Fluorescent Protein
UseYeast taggingTagsExpressionMutationPromoterAvailable sinceOct. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFA6-mTFP1-KanMX6
Plasmid#69851PurposeC-terminal tagging with mTFP1 using KanMX6 as a selection marker in S. cerevisiae.DepositorInsertmTFP1
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 16, 2018AvailabilityAcademic Institutions and Nonprofits only