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Showing: 1101 - 1150 of 147948 results

  2. pAAV-hSyn-hM4D(Gi)-mCherry

    Plasmid
    #50475
    Purpose
    Gi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.
    Depositor
    Bryan Roth
    Has Service
    AAV Retrograde, AAV2, AAV5, AAV8, and AAV9
    Insert
    hM4D(Gi)-mCherry
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50475-plasmid-map-sequence-id-171072

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  5. pCMV delta R8.2

    Plasmid
    #12263
    Depositor
    Didier Trono
    Insert
    HIV-1 GAG/POL, Tat and Rev
    Use
    Lentiviral; Packaging
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12263-plasmid-map-sequence-id-394974

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  8. CRISPRoff-v2.1

    Plasmid
    #167981
    Purpose
    Expresses CRISPRoff-v2.1 (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB) downstream of the CAG promoter
    Depositor
    Luke Gilbert
    Article
    Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003
    Insert
    CRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)
    Use
    Tags
    2xNLS, DNMT3A-DNMT3L, HA, KRAB, and tagBFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167981-plasmid-map-sequence-id-330241

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  9. pCS2+MLS-HyPer7

    Plasmid
    #136470
    Purpose
    Mammalian expression of mitochondrial matrix targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging
    Depositor
    Vsevolod Belousov
    Article
    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.
    Insert
    HyPer7
    Use
    Tags
    Tandem mitochondrial targeting signal of cytochro…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, SP6
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    136470-plasmid-map-sequence-id-271540

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  11. pAAV-CAG-GFP

    Plasmid
    #37825
    Depositor
    Edward Boyden
    Has Service
    AAV CAP-B10, AAV CAP-B22, AAV MaCPNS1, AAV MaCPNS2, AAV PHP.eB, AAV Retrograde, AAV Retrograde trial size, AAV1, AAV1 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV8, AAV8 trial size, AAV9, and AAV9 trial size
    Insert
    GFP
    Use
    AAV; Adeno-associated virus
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CAG
    Available since
    Aug. 8, 2012
    Availability
    Academic Institutions and Nonprofits only
    37825-plasmid-map-sequence-id-402523

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  14. Lamp1-RFP

    Plasmid
    #1817
    Purpose
    Mammalian expression of Lamp1 fused to RFP. Used for localization to the lysosome.
    Depositor
    Walther Mothes
    Article
    Sherer et al Traffic 2003 Nov;4(11):785-801.
    Insert
    lysosome associated membrane protein 1 (Lamp1 Rat)
    Use
    Tags
    RFP
    Expression
    Mammalian
    Mutation
    D50E (not important for function of plasmid)
    Promoter
    Available since
    Sept. 26, 2005
    Availability
    Academic Institutions and Nonprofits only
    1817-plasmid-map-sequence-id-350820

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  23. pM1s3AsG

    Plasmid
    #137921
    Purpose
    E. coli Nissle pMUT1-derived plasmid with ampicillin resistance and constitutive GFP expression
    Depositor
    Neel Joshi
    Article
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Insert
    Superfolder GFP
    Use
    Synthetic Biology; For use with e. coli nissle in…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23101
    Available since
    Jan. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    137921-plasmid-map-sequence-id-322876

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  24. pcDNA3-YFP

    Plasmid
    #13033
    Purpose
    Mammalian expression vector for cloning your gene fused to YFP
    Depositor
    Doug Golenbock
    Has Service
    Cloning Grade DNA
    Insert
    Yellow fluorescent protein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13033-plasmid-map-sequence-id-210331

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  25. pComb3XTT

    Plasmid
    #63891
    Purpose
    Backbone of pComb3X containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control and PCR template for human antibody constant regions (kappa and CH1)
    Depositor
    Carlos Barbas
    Article
    Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):
    Insert
    TT Fab
    Use
    Phage display
    Tags
    6xHis, HA tag, and geneIII
    Expression
    Bacterial
    Mutation
    Promoter
    lacZ
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63891-plasmid-map-sequence-id-245916

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  28. pcDNA3-mRuby2

    Plasmid
    #40260
    Purpose
    RFP with robust performance in protein fusions and useful as FRET acceptor for Clover in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap
    Depositor
    Michael Lin
    Article
    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm
    Has Service
    Cloning Grade DNA
    Insert
    mRuby2
    Use
    Tags
    His6 and Xpress
    Expression
    Mammalian
    Mutation
    Promoter
    CMV IE
    Available since
    Sept. 20, 2012
    Availability
    Academic Institutions and Nonprofits only
    40260-plasmid-map-sequence-id-243381

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  33. pBS-CMV-gagpol

    Plasmid
    #35614
    Depositor
    Patrick Salmon
    Insert
    MLV gag and pol genes
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 12, 2012
    Availability
    Academic Institutions and Nonprofits only
    35614-plasmid-map-sequence-id-339908

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  34. eeBxb1_IVT

    Plasmid
    #222342
    Purpose
    Plasmid for evolved and engineered Bxb1 mRNA in vitro transcription template
    Depositor
    David Liu
    Article
    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103
    Insert
    Codon-optimized evolved and engineered Bxb1
    Use
    Template for in vitro transcription
    Tags
    Expression
    Mutation
    Promoter
    T7 (inactivated)
    Available since
    July 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    222342-plasmid-map-sequence-id-440249

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  46. pcDNA3-EGFP

    Plasmid
    #13031
    Purpose
    Mammalian expression vector for cloning your gene fused to EGFP
    Depositor
    Doug Golenbock
    Has Service
    Cloning Grade DNA
    Insert
    Enhanced Green Fluorescent Protein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13031-plasmid-map-sequence-id-305137

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Showing: 1101 - 1150 of 147948 results