We narrowed to 4,591 results for: ado

Showing: 881 - 920 of 4591 results

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PX458_MIXL1_2

Plasmid

#86336

Purpose

Encodes gRNA for 3' target of human MIXL1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against MIXL1 (MIXL1 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_MIXL1_1

Plasmid

#86335

Purpose

Encodes gRNA for 3' target of human MIXL1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against MIXL1 (MIXL1 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_ZNF580_2

Plasmid

#86332

Purpose

Encodes gRNA for 3' target of human ZNF580

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ZNF580 (ZNF580 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_ZNF580_1

Plasmid

#86331

Purpose

Encodes gRNA for 3' target of human ZNF580

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ZNF580 (ZNF580 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_ZSCAN9_2

Plasmid

#86313

Purpose

Encodes gRNA for 3' target of human ZSCAN9

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ZSCAN9 (ZNF193 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_SAP130_2

Plasmid

#86311

Purpose

Encodes gRNA for 3' target of human SAP130

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against SAP130 (SAP130 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_ZSCAN9

Plasmid

#86268

Purpose

Donor vector for 3' FLAG tag of human ZSCAN9

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZSCAN9 homology arms (ZNF193 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pHL1633

Plasmid

#62830

Purpose

Lim Lab plasmid: AmpR + PLlacO-1::T710 gfp::Asp terminator + ColE1

Depositor

Han Lim

Article

Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

Insert

gfp

Use

Synthetic Biology

Tags

T710

Expression

Bacterial

Promoter

pLlacO-1

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only
pCVD403

Plasmid

#63740

Purpose

Contains the LT gene probe (HincII fragment) cloned on BamHI linkers into pACYC184

Depositor

James Kaper

Article

Gicquelais et al J Clin Microbiol. 1990 Nov;28(11):2485-9

Insert

LT gene probe

Expression

Bacterial

Available Since

April 6, 2015

Availability

Academic Institutions and Nonprofits only
M26L DJ-1 pET15b

Plasmid

#60679

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Lakshminarasimhan et al Biochemistry. 2008 Feb 5;47(5):1381-92.

Insert

M26L DJ-1 (PARK7 Human)

Tags

6xHis

Expression

Bacterial

Mutation

M26L

Promoter

T7

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pCK01_PalkS_alkS

Plasmid

#166502

Purpose

Expression of AlkS transcriptional regulator with L401G mutation. Sensor input plasmid for detection of n-butanol and branched-chain higher alcohols.

Depositor

Sven Panke

Article

Bahls et al Metab Eng. 2021 Nov 9. pii: S1096-7176(2

Insert

alkS

Use

Synthetic Biology

Expression

Bacterial

Mutation

L401G

Promoter

PalkS

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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EF1a_mCherry_P2A_Hygro_Barcode

Plasmid

#120426

Purpose

Barcoded lentiviral vector to express mCherry in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

mCherry

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-mTH-GFP

Plasmid

#99128

Purpose

An AAV genome that expresses GFP from the mouse TH promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

GFP

Use

AAV

Expression

Mammalian

Promoter

mTH

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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Fuw-dCas9-Tet1CD

Plasmid

#84475

Purpose

Lentiviral construct to express dCas9 fused with the catalytic domain of Tet1

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Tet1CD

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pBMN(CMV-copGFP-Puro-SMAR)

Plasmid

#80391

Purpose

expression copGFP and Puromycine, with an S/MAR element for episomal maintaince

Depositor

Magnus Essand

Article

Jin et al EMBO Mol Med. 2016 May 6. pii: e20150586

Insert

copGFP-T2A-Puromycine

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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M-NylC_pET28a

Plasmid

#215409

Purpose

Expression construct for M-NylC gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

M-NylC

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-mNeonGreen

Plasmid

#99131

Purpose

An AAV genome with tet-inducible expression of the fluorescent protein mNeonGreen

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mNeonGreen

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pRS156

Plasmid

#182707

Purpose

CRISPRa plasmid for Candida albicans (dCas9-VPR and sgRNA cloning site)

Depositor

Rebecca Shapiro

Article

Gervais et al G3 (Bethesda). 2022 Dec 1:jkac301. doi:

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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B52_puro_empty_gRNA

Plasmid

#197557

Purpose

sites for cloning two gRNA with puromycin cassette

Depositor

Darius Bägli

Type

Empty backbone

Use

Empty grna backbone with puromycin resistance gene

Expression

Mammalian

Promoter

U6 for gRNA, CMV for puromycin resistance.

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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Fuw-dCas9-Tet1CD_IM

Plasmid

#84479

Purpose

Lentiviral construct to express dCas9 fused with an inactive catalytic domain of Tet1 (H1672Y, D1674A)

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Tet1CD_IM

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pJH-MBP-CbAgo

Plasmid

#127707

Purpose

pET His6 MBP TEV CbAgo

Depositor

John van der Oost

Article

Hegge et al Nucleic Acids Res. 2019 May 9. pii: 5487

Insert

CbAgo

Tags

MPB

Expression

Bacterial

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pFETCh_RXRB

Plasmid

#104040

Purpose

Donor vector for 3' FLAG tag of human RXRB

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

RXRB homology arms (RXRB Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mammalian

Available Since

Feb. 23, 2018

Availability

Academic Institutions and Nonprofits only

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Fuw-dCas9-Dnmt3a_IM

Plasmid

#84478

Purpose

Lentiviral construct to express dCas9 fused with an inactive Dnmt3a catalytic domain (E664A, E756A)

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Dnmt3a_IM

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-TREX1(D18N)

Plasmid

#27220

Depositor

Judy Lieberman

Article

Yan et al Nat Immunol. 2010 Nov . 11(11):1005-13.

Insert

TREX1 (TREX1 Human)

Tags

GFP

Expression

Mammalian

Mutation

D18N sequence 52-GAC changed to AAC, resulting i…

Available Since

Jan. 26, 2011

Availability

Academic Institutions and Nonprofits only

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NylCK-TS_pET28a

Plasmid

#215423

Purpose

Expression construct for NylCK-TS gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylCK-TS

Expression

Bacterial

Mutation

S111G/A137L

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-mRuby2

Plasmid

#99114

Purpose

An AAV genome with tet-inducible expression of the fluorescent protein mRuby2

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mRuby2

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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PB-5'-ENGRAM

Plasmid

#179157

Purpose

piggyBac plasmid for 5' ENGRAM recorder

Depositor

Jay Shendure

Article

Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

minP

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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piggyBAC-5xTAPE-1-BlastR

Plasmid

#175808

Purpose

Encodes 5xTAPE-1 construct that can be integrated using piggyBAC system

Depositor

Jay Shendure

Article

Choi et al Nature. 2022 Jul 6. pii: 10.1038/s41586-

Insert

5xTAPE-1 construct

Expression

Mammalian

Promoter

U6

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
ReU_pET29b

Plasmid

#215426

Purpose

Expression construct for ReU gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

ReU

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
NylCA-TS_pET28a

Plasmid

#215419

Purpose

Expression construct for NylCA-TS gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylCA-TS

Expression

Bacterial

Mutation

S111G/A137L/E263Q

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Adeno Cas9

Plasmid

#64072

Purpose

Adenoviral vector for the expression of the Cas9

Depositor

Andrea Ventura

Article

Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

Type

Empty backbone

Use

Adenoviral

Tags

FLAG

Promoter

U6 and CBh

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
scFLARE1

Plasmid

#158738

Purpose

Mammalian expression of scFLARE1 component

Depositor

Alice Ting

Article

Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

Insert

scFLARE1

Use

AAV

Tags

Flag, V5

Expression

Mammalian

Mutation

Truncated form of TEV protease (220-242 amino aci…

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA 3.1 Flag-rat-PTP1B

Plasmid

#35988

Depositor

Anna Huttenlocher

Article

Cortesio et al J Cell Biol. 2008 Mar 10;180(5):957-71.

Insert

protein tyrosine phosphatase 1B (Ptpn1 Rat)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

June 1, 2012

Availability

Academic Institutions and Nonprofits only

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pLJC6-NADK2-3xFLAG

Plasmid

#233895

Purpose

lentiviral overexpression vector for NADK2

Depositor

Jason Cantor

Article

Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

Insert

NADK2 (NADK2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

Ubc

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
P023-mPB-synHEK3-BsaI

Plasmid

#239975

Purpose

PiggyBac plasmid for synthetic DNA Tape (HEK3 locus)

Depositor

Jay Shendure

Article

Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-Dlx-tTA

Plasmid

#210730

Purpose

AAV vector expressing tetracycline-controlled transactivator (tTA) under Dlx promoter (for expressing tTA in GABAergic neurons)

Depositor

Wei Xu

Article

Thompson et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

tetracycline-controlled transactivator (tTA)

Use

AAV

Promoter

Dlx

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
NylCp2-TS_pET28a

Plasmid

#215421

Purpose

Expression construct for NylCp2-TS gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylCp2-TS

Expression

Bacterial

Mutation

D122G/H130Y/D36A/E263Q

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pY2F

Plasmid

#53285

Purpose

Contains an Arabidopsis thaliana lcyE cDNA and produces an active lycopene epsilon-cyclase enzyme in E. coli. Use with pAC-BETA-At or pAC-BETAipi-At to produce alpha-carotene in E. coli.

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

Insert

lcyE (LUT2 Mustard Weed)

Expression

Bacterial

Promoter

lac

Available Since

April 16, 2015

Availability

Academic Institutions and Nonprofits only

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pLJC2-Blast-SHMT2-3xFLAG

Plasmid

#233891

Purpose

lentiviral overexpression vector for SHMT2

Depositor

Jason Cantor

Article

Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

Insert

SHMT2 (SHMT2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC103-PBNeo-U6-sgRNA-Expression

Plasmid

#48228

Purpose

sgRNA expression on PiggyBac vector with Neo-selectable marker

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

April 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 4,591 results for: ado