Addgene

Showing: 441 - 460 of 150368 results

Results per page:

Sort results by:

pcDNA3 Flag Erg

Plasmid

#66977

Purpose

Expression plasmid for mouse Erg; adds Flag tag to the N-terminus of protein

Depositor

Christopher Vakoc

Article

Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

Insert

Erg (Erg Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only
pBUE411-GG

Plasmid

#198232

Purpose

Wheat GRF4-GIF1 chimera in pBUE411

Depositor

Andrea Gallavotti

Insert

GRF4-GIF1 chimera cassette

Use

CRISPR; Plant binary vector

Tags

Expression

Plant

Mutation

Promoter

Ubiquitin

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDFDuet-nsp7-nsp8

Plasmid

#159092

Purpose

Coexpression construct of Nsp7 with an N-terminal His-tag and Nsp8

Depositor

Seth Darst

Article

Chen et al bioRxiv. 2020 Jul 13. doi: 10.1101/2020.

Inserts

NSP7
NSP8

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Puro-iNotch1IC

Plasmid

#75338

Purpose

doxycycline inducible Notch1 intracellular domain gene over-expression

Depositor

Danwei Huangfu

Article

Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

Insert

Notch1 Intracellular domain (Notch1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE-tight

Available since

May 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
E228Q Vps4A-HA

Plasmid

#200087

Purpose

3x HA-VPS4A with E228Q dominant negative mutation in Dox-inducible pInducer20 vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

VPS4A (VPS4A Human)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

E228Q

Promoter

CMV

Available since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB444

Plasmid

#74830

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with eCFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-myc-EFNB2 (WT)

Plasmid

#200975

Purpose

Mammalian expression plasmid for wild type, myc-tagged EFNB2

Depositor

Erik Procko

Article

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EFNB2 (EFNB2 Human)

Use

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-SIGLEC7-Fc(DAPA)-AviTag-6xHis

Plasmid

#156773

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SIGLEC7 (SIGLEC7 Human)

Use

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pYLsgRNA-OsU6a/LacZ

Plasmid

#66195

Purpose

cloning of sgRNAs for expression in plants

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

OsU6a

Available since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJC6-HK2-3xFLAG

Plasmid

#163451

Purpose

lentiviral overexpression vector for human HK2

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

HK2 (HK2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

See: comments

Promoter

Ubc

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJSC111 - Bacterial expression plasmid for SpCas9-HF1 variant

Plasmid

#101209

Purpose

Bacterial expression plasmid for SpCas9-HF1 variant

Depositor

Keith Joung, Jennifer Doudna

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant N497A/R661A/Q695A/Q926A

Use

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

N497A, R661A, Q695A and Q926A

Promoter

T7

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-HA-deLACCO1-NGR

Plasmid

#208097

Purpose

Control biosensor for extracellular L-lactate

Depositor

Marie-Eve Paquet, Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

deLACCO1

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-gfaABC1D-HA-deLACCO1-NGR

Plasmid

#208099

Purpose

Control biosensor for extracellular L-lactate

Depositor

Marie-Eve Paquet, Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

R-iLACCO1.1

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pJAT75

Plasmid

#204306

Purpose

Flippase under heat shock control. HDR integrant marked with ie1-EGFP, expressed in abdomen and mouthparts

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

ie1-EGFP

Use

CRISPR

Tags

Expression

Mutation

Promoter

ie1

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Epsin1-ECFP

Plasmid

#21066

Depositor

Xiaowei Zhuang

Article

Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

Insert

Epsin 1 (Epn1 Rat)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2009

Availability

Academic Institutions and Nonprofits only
pCMV-PL

Plasmid

#20783

Depositor

Bryan Cullen

Article

Zeng et al Mol Cell. 2002 Jun . 9(6):1327-33.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2009

Availability

Academic Institutions and Nonprofits only
Thy1.1-GFP

Plasmid

#131703

Purpose

encodes Thy1.1 followed by a P2A site and GFP for enriching pure populations of transfected cells

Depositor

Britt Glaunsinger

Article

Gilbertson et al Elife. 2018 Oct 3;7. pii: 37663. doi: 10

Insert

Thy1.1-GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-mCherry-tSH2(ZAP70)

Plasmid

#27137

Depositor

Ron Vale

Article

Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

Insert

mCherry and ZAP70 (residues 1-259) (ZAP70 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

April 12, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330-LMNA-gRNA1

Plasmid

#122507

Purpose

expresses WT spCas9 and a chimeric gRNA targeting human LMNA

Depositor

Graham Dellaire

Article

Pinder et al Nucleic Acids Res. 2015 Oct 30;43(19):93

Insert

LMNA-gRNA1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVTH-sip53

Plasmid

#12239

Depositor

Didier Trono

Article

Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

Insert

shRNA against p53 (TP53 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge