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pcDNA5-Flag-Adrm1

Plasmid

#19417

Depositor

Ronald Conaway, Joan Conaway

Article

Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

Insert

adhesion regulating molecule 1 (ADRM1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pRham-CALM1

Plasmid

#182500

Purpose

Bacterial expression of the Homo sapiens calmodulin, CALM1

Depositor

Dan Mulvihill

Article

Baker et al FEBS Lett. 2022 Jan 31. doi: 10.1002/187

Insert

CALM1 (CALM1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

rhamnose

Available since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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pJT301 BxBi Lenti pEF-attB LSR GFP

Plasmid

#184944

Purpose

Lentiviral delivery of recombinase landing pad

Depositor

Patrick Hsu, Lacra Bintu, Ami Bhatt, Michael Bassik

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

BxBi Lenti pEF1a-attB LSR GFP

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-ITSN2

Plasmid

#129615

Purpose

RhoGEF

Depositor

Dorus Gadella

Article

Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

Insert

ITSN2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pJC005.gent

Plasmid

#182739

Purpose

CRISPR-Cas12a genome editing of E. faecium

Depositor

James Collins

Article

Chua et al Microbiol Spectr. 2022 Feb 2:e0242721. d

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pBABE.is4-mOpn

Plasmid

#74410

Purpose

Expresses mouse Osteopontin from transcript tv4 in mammalian cells

Depositor

Georgios Stathopoulos

Insert

mouse Opn (Spp1 Mouse)

Use

Tags

none

Expression

Mammalian

Mutation

Promoter

MMLV

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR-ABCC1_STOP

Plasmid

#221423

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Wolf et al Cell Chem Biol. 2024 Dec 24:S2451-9456(2

Insert

ABCC1 (ABCC1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pET11a-4SS-HEWL

Plasmid

#39233

Depositor

Harald Schwalbe

Article

Schlorb et al J Biomol NMR. 2005 Oct;33(2):95-104.

Insert

Hen egg white lysozyme (LYZ Chicken)

Use

Tags

Expression

Bacterial

Mutation

N-terminal Met

Promoter

T7

Available since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-mSncg-Cre

Plasmid

#153207

Purpose

Mouse gamma-synuclein (mSncg) promoter-mediates Cre expression in retinal ganglion cells

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Insert

Cre

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

mSncg

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pIRES-hrGFP II-TET1

Plasmid

#83568

Purpose

Expresses TET1 in mammalian cells

Depositor

Jean-Pierre Issa

Article

Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

Insert

Tet Methylcytosine Dioxygenase 1 (TET1 Human)

Use

Tags

3X FLAG tag and hr GFP II

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pRK6-HA-TAK1

Plasmid

#44160

Depositor

Xin Lin

Article

Blonska et al J Biol Chem. 2005 Dec 30;280(52):43056-6

Insert

Tak1 (Map3k7 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 9, 2013

Availability

Academic Institutions and Nonprofits only
FKBP-epsin(144-575)-GFP

Plasmid

#100733

Purpose

To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

epn1 (Epn1 Mouse)

Use

Tags

FKBP and GFP

Expression

Mammalian

Mutation

clathrin binding middle part aa 144-575

Promoter

CMV

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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PE-P3-rice

Plasmid

#185612

Purpose

For plant prime editing in rice plants

Depositor

Jinxiao Yang

Article

Xu et al Nat Plants. 2022 Jan;8(1):45-52. doi: 10

Insert

3XFLAG-4XNLS-M-MLV-nCas9(H840A)-4XNLS-P2A-Hyg

Use

CRISPR

Tags

Expression

Mutation

Promoter

ZmUbi

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAW014-2

Plasmid

#85612

Purpose

Multi-gRNA delivery vector containing ugtP-gRNA.P395T for Bacillus subtilis

Depositor

Perry Chou

Article

Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

Insert

ugtP-gRNA.P395T

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCI-neo-RL-ZEB1

Plasmid

#35535

Depositor

Greg Goodall

Article

Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

Insert

3'UTR of ZEB1 (ZEB1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2012

Availability

Academic Institutions and Nonprofits only

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pSK-mU6-Cj-sgRNA-SV40-puro

Plasmid

#192128

Purpose

Expresses Cj-SgRNA scaffold cloned with BbsI and puromycin resistance gene

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Cj-SgRNA scaffold

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pIRESpuroN3xFLAG-His-CDK5RAP3

Plasmid

#204555

Purpose

Expresses 3xFLAG-His-tagged CDK5RAP3 in mammalian cells

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

CDK5RAP3 (AMY2A Human)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCI-neo-RL-ZEB2

Plasmid

#35536

Depositor

Greg Goodall

Article

Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

Insert

3'UTR of ZEB2 gene (ZEB2 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

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Mucolipin1-pHcRed C1

Plasmid

#62959

Purpose

full length human Mucolipin-1 cloned into pHcRed1 C1

Depositor

Paul Luzio

Article

Pryor et al Traffic. 2006 Oct;7(10):1388-98.

Insert

Mucolipin-1 (MCOLN1 Human)

Use

Tags

HcRed1

Expression

Mammalian

Mutation

Mucolipin-1 has 1 silent mutation compared to the…

Promoter

CMV

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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Mucolipin1 D471-472K-pHcRed C1

Plasmid

#62961

Purpose

full length human Mucolipin-1 D471/472K cloned into pHcRed1 C1

Depositor

Paul Luzio

Article

Pryor et al Traffic. 2006 Oct;7(10):1388-98.

Insert

Mucolipin-1 (MCOLN1 Human)

Use

Tags

HcRed

Expression

Mammalian

Mutation

D471/472K (and two silent mutations T77 ACA inst…

Promoter

CMV

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only