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Showing: 11401 - 11450 of 28579 results

  1. pEF NS34a-msGFP-'Q-mito

    Plasmid
    #158764
    Purpose
    Expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
    Depositor
    Edwin Chapman
    Article
    Vevea et al Elife. 2020 Jun 9;9. pii: 56469. doi: 10
    Insert
    NS3/4a protease and msGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Flexible linkers between the protease and msGFP a…
    Promoter
    Available since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    158764-plasmid-map-sequence-id-312950

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  4. pQZ210

    Plasmid
    #106414
    Purpose
    Co-expresses trans SNARE complex with pDC183 in E.coli
    Depositor
    Axel Brunger
    Article
    Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d
    Inserts
    Rat Synaptobrevin-2 fragment 28-66 (Vamp2 Synthetic)
    Syntaxin-1A fragment 191-256 (Stx1a Rat, Synthetic)
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Fragment 191-256 , Codon optimization and Fragmen…
    Promoter
    T7
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    106414-plasmid-map-sequence-id-204167

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  11. HSC1-D4Z4-GiP

    Plasmid
    #58542
    Purpose
    Derived from HSC1-GiP where the D4Z4 insulator is cloned upstream of the EF1 alpha promoter
    Depositor
    James Ellis
    Article
    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1
    Inserts
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    D4Z4 repeat, partial
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human EF1-alpha
    Available since
    Sept. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    58542-plasmid-map-sequence-id-90651

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  17. p3E-H2B-mCerulean3-P2A-mKOFP2-CAAX

    Plasmid
    #75177
    Purpose
    Nuclear mCerulean3 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with C-terminal fusions.
    Depositor
    Nicholas Cole
    Article
    Don et al Zebrafish. 2016 Sep 15.
    Insert
    H2B-mCerulean3-P2A-mKOFP2-CAAX
    Use
    Three prime entry vector for multisite gateway th…
    Tags
    Expression
    Mutation
    Promoter
    n/a
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    75177-plasmid-map-sequence-id-145999

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  19. LITE1.0_pAAV_hSyn_CRY2PHR-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

    Plasmid
    #47454
    Purpose
    epiLITE / LITE1.0 CRY2PHR fused to SID4X transcriptional repressor effector domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    CRY2PHR-NLS-SID4X
    Use
    AAV
    Tags
    2A_phiLOV2.1
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Available since
    Oct. 15, 2013
    Availability
    Academic Institutions and Nonprofits only
    47454-plasmid-map-sequence-id-69624

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  22. pBI101-pGC1(D1)-GUS

    Plasmid
    #49513
    Purpose
    Expresses beta-glucuronidase gene under the control of guard cell promoter (pGC1 (D1)) in plant
    Depositor
    Julian Schroeder
    Article
    Yang et al Plant Methods. 2008 Feb 19;4:6. doi: 10.
    Insert
    Promoter of GC1(D1) (AT1G22690 Mustard Weed)
    Use
    Plant expression
    Tags
    beta-glucuronidase
    Expression
    Mutation
    Promoter
    GC1 (At1g22690), 1141 bp upstream of the transcri…
    Available since
    Jan. 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  24. DsRed +9e

    Plasmid
    #191766
    Purpose
    Expression of DsRed fused to a positively charged polypeptide (2 x KSG) . Overall charge on tetramer: +9e
    Depositor
    Daniel Gerlich
    Article
    Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:
    Insert
    DsRed
    Use
    Tags
    N terminal fusion of positively charged polypetod…
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    191766-plasmid-map-sequence-id-383844

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  31. pAAV-hSyn-LMO4 (GLucM23-VChR1-EYFP)

    Plasmid
    #114101
    Purpose
    fusion protein of Gaussia luciferase variant M23, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics
    Depositor
    Ute Hochgeschwender
    Article
    Park et al J Neurosci Res. 2017 Sep 1. doi: 10.1002
    Inserts
    Gaussia luciferase, M23
    Volvox Channelrhodopsin 1
    Enhanced Yellow Fluorescent Protein
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Aug. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    114101-plasmid-map-sequence-id-252522

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  32. XW17

    Plasmid
    #65835
    Purpose
    Punc-4-QF-3'UTR-SL2-mCherry-unc-54 (The endogenous SphI, KpnI and SacI sites in QF are removed for the convenience of cloning, most sites are compatible with pPD95.77)
    Depositor
    Kang Shen
    Article
    Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi
    Insert
    OR74A quinic acid utilization activator (qa-1F)
    Use
    Tags
    mCherry
    Expression
    Worm
    Mutation
    The endogenous SphI, KpnI and SacI sites in QF ar…
    Promoter
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    65835-plasmid-map-sequence-id-120565

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  37. PB-eDIO-ChR2-EYFP

    Plasmid
    #104387
    Purpose
    PiggyBac plasmid encoding channelrhodopsin 2 fused to EYFP in an inverse orientation flanked by loxP and lox2722 sites under the control of the EF1a promotor.
    Depositor
    Volker Busskamp
    Article
    Klapper et al Sci Rep. 2017 Oct 31;7(1):14450. doi: 10
    Insert
    Channelrhodopsin 2
    Use
    Cre/Lox; Piggybac
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    E134R
    Promoter
    Available since
    Feb. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    104387-plasmid-map-sequence-id-203589

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  38. pDC183

    Plasmid
    #106415
    Purpose
    Co-expresses trans SNARE complex with pQZ210 in E. coli
    Depositor
    Axel Brunger
    Article
    Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d
    Inserts
    SNAP25 fragment 141-204 (Snap25 Rat, Synthetic)
    SNAP25 Fragment 7-83 (Snap25 Rat, Synthetic)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Fragment 141-204, Codon optimization and Fragment…
    Promoter
    T7
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    106415-plasmid-map-sequence-id-204169

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Showing: 11401 - 11450 of 28579 results