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TBPL1 KO 2

Plasmid

#200211

Purpose

gRNA for TBPL1 KO

Depositor

Sheila Teves

Article

Kwan et al Elife. 2023 Mar 30;12:e83810. doi: 10.75

Insert

TBP1 KO 2 (Tbpl1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

U6

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pcoCASphi_E9t_V2_CmYLCVp_35sT_ribozyme_AtPDS3_gRNA10

Plasmid

#197958

Purpose

T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter and a single AtPDS3 gRNA driven by CmYLCVp and flanked by ribozymes.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Inserts

pcoCasphi-2
AtPDS3 gRNA10

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

CmYLCVp and pUBQ10

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pco-vCASphi_E9t_V2_U6_AtPDS3_gRNA10

Plasmid

#197963

Purpose

T-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by U6 promoter.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Inserts

pco-vCasphi-2
AtPDS3 gRNA10

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

AtU6-26 and pUBQ10

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pTij_sg_mmu_Med6_C_terminal

Plasmid

#197869

Purpose

CRISPR/Cas9/sgRNA plasmid for cutting Med6 C terminal and building MED6-mEmerald/Halo knock-in mESCs

Depositor

James Zhe Liu

Article

Dong et al bioRxiv 2022.10.26.513917

Insert

Med6 (Med6 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pClgn-Tesmin-BioID2-3xFLAG

Plasmid

#186816

Purpose

Mouse Clgn promoter-driven expression of TESMIN-BioID2-3xFLAG

Depositor

Masahito Ikawa

Article

Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

Insert

Tesmin (Tesmin Mouse)

Use

Tags

BioID2-3xFLAG

Expression

Mammalian

Mutation

Promoter

mouse Clgn promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pClgn-Tesmin-TurboID-3xFLAG

Plasmid

#186817

Purpose

Mouse Clgn promoter-driven expression of TESMIN-TurboID-3xFLAG

Depositor

Masahito Ikawa

Article

Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

Insert

Tesmin (Tesmin Mouse)

Use

Tags

TurboID-3xFLAG

Expression

Mammalian

Mutation

Promoter

mouse Clgn promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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UAS-GFP::NMN-Deamidase{dead}

Plasmid

#187869

Purpose

Gal4/UAS expression of GFP::NMN-Deamidase(dead)

Depositor

Lukas Neukomm

Article

Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

Insert

NMN-Deamidase{dead}

Use

Tags

GFP

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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UAS-4x(tRNA::axed{sgRNA})

Plasmid

#187883

Purpose

Gal4/UAS sgRNA expression targeting axed

Depositor

Lukas Neukomm

Article

Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

Insert

4 sgRNAs targeting axed

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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UAS-4x(tRNA::hiw{sgRNA})

Plasmid

#187884

Purpose

Gal4/UAS sgRNA expression targeting hiw

Depositor

Lukas Neukomm

Article

Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

Insert

4 sgRNAs targeting hiw

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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UAS-4x(tRNA::dsarm{sgRNA})

Plasmid

#187885

Purpose

Gal4/UAS sgRNA expression targeting dsarm

Depositor

Lukas Neukomm

Article

Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

Insert

4 sgRNAs targeting dSarm

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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1137B=Hsp70Bb-Cas9

Plasmid

#153284

Purpose

The attB plasmid harboring the Hsp70Bb-Cas9-T2A-eGFP-p10 and a mini-white marker.

Depositor

Omar Akbari

Article

Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

Insert

pHsp70Bb-SpCas9-T2A-eGFP-p10 (cas9 Synthetic)

Use

CRISPR

Tags

eGFP

Expression

Insect

Mutation

Promoter

Dmel Hsp70Bb

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19-SpRYgest_targetplasmid (KAC833)

Plasmid

#181748

Purpose

pUC19 derivative plasmid used as a substrate for SpRYgest, restriction enzyme, and TtAgo digests. Encodes an SpCas9 EMX1-site1-NGG-PAM target site between NheI and HindIII restriction sites.

Depositor

Benjamin Kleinstiver

Article

Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

Type

Empty backbone

Use

Substrate plasmid

Tags

Expression

Mutation

Promoter

Available since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

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BPK1179

Plasmid

#179296

Purpose

Expresses dCas9 fused to four DmrA domains

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

Insert

dCas9-DmrA(x4)

Use

Tags

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive Cas9)

Promoter

CAG

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pBI101.1/ProGOXL6:GUS

Plasmid

#175562

Purpose

Binary vector for Agrobacterium-mediated plant transformation - Promoter:GUS reporter for expression analysis of GOXL genes

Depositor

George Haughn

Article

Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

Insert

ProGOXL6

Use

Tags

GUS

Expression

Plant

Mutation

Promoter

GOXL6

Available since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pBI101.1/ProGOXL5:GUS

Plasmid

#175561

Purpose

Binary vector for Agrobacterium-mediated plant transformation - Promoter:GUS reporter for expression analysis of GOXL genes

Depositor

George Haughn

Article

Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

Insert

ProGOXL5

Use

Tags

GUS

Expression

Plant

Mutation

Promoter

GOXL5

Available since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pBI101.1/ProGOXL4:GUS

Plasmid

#175560

Purpose

Binary vector for Agrobacterium-mediated plant transformation - Promoter:GUS reporter for expression analysis of GOXL genes

Depositor

George Haughn

Article

Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

Insert

ProGOXL4

Use

Tags

GUS

Expression

Plant

Mutation

Promoter

GOXL4

Available since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pBI101.1/ProGOXL3:GUS

Plasmid

#175559

Purpose

Binary vector for Agrobacterium-mediated plant transformation - Promoter:GUS reporter for expression analysis of GOXL genes

Depositor

George Haughn

Article

Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

Insert

ProGOXL3

Use

Tags

GUS

Expression

Plant

Mutation

Promoter

GOXL3

Available since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pBI101.1/ProGOXL2:GUS

Plasmid

#175558

Purpose

Binary vector for Agrobacterium-mediated plant transformation - Promoter:GUS reporter for expression analysis of GOXL genes

Depositor

George Haughn

Article

Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

Insert

ProGOXL2

Use

Tags

GUS

Expression

Plant

Mutation

Promoter

GOXL2

Available since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pAT15484_HF-ABE8e

Plasmid

#174122

Purpose

Expresses ABE8e fused with SpCas9-HF1

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

HF-ABE8e

Use

CRISPR

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Mammalian

Mutation

Promoter

EF1-a

Available since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR207-MUM2sp-PelA

Plasmid

#170722

Purpose

Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the HG degrading enzyme PelA (AN2331.2)

Depositor

George Haughn

Article

McGee et al Plant Cell Physiol. 2021 Jun 1. pii: 629

Insert

Pectin lyase A

Use

Gateway donor vector / entry clone

Tags

Expression

Mutation

Promoter

Available since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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