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Plasmid#126955PurposeBacterial expression plasmid for eGFP-(SIM)3 client to assess SUMO-SIM scaffolds.DepositorInsertEGFP-SUMO interacting motif (SIM) from PIASx (PIAS2 Human)
UseTagseGFPExpressionBacterialMutationPromoterunknownAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-H3.3-polyA
Plasmid#59780PurposeExpress Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.DepositorInsertH3F3B (H3-3B Human)
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceSept. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-GpNLuc_SV40:PuroR
Plasmid#183037PurposeExpresses the FLAG-tagged GpNLuc (Green LumiFluor) in mammalian cells.DepositorInsertGpNLuc LumiFluor
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
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GFP-(SIM)1
Plasmid#126954PurposeBacterial expression plasmid for eGFP-(SIM)1 client to assess SUMO-SIM scaffolds.DepositorInsertEGFP-SUMO interacting motif (SIM) from PIASx (PIAS2 Human)
UseTagseGFPExpressionBacterialMutationPromoterunknownAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDuRCC-K
Plasmid#81193PurposeExpression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan ResistanceDepositorInsertsCas9
empty gRNA cassette
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease see depositor comments below., Please view…PromoterROX3 and SNP52pAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCG-TPMV Fd32
Plasmid#163964PurposeExpression of TPMV F glycoprotein in mammalian cellsDepositorInsertTPMV F glycoprotein
UseTagsExpressionMammalianMutationLacking 32 amino acids at C-terminusPromoterAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
KA2628_pGL4-UBCmin-luc2/Tbx3-B'
Plasmid#124201PurposeLuciferase reporter driven by the Tbx3 enhancerDepositorInsertTbx3 enhancer
UseLuciferaseTagsExpressionMutationPromoterUBCminAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA1153_pPBCAG-MerCreMer-IN
Plasmid#124183PurposepiggyBac-based expression of the tamoxifen-inducible CreDepositorInsertMerCreMer-IRES-Neo
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-CaMKKbeta
Plasmid#32453DepositorInsertCalcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)
UseTagsFLAG and GFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-GeNL_SV40:PuroR
Plasmid#183040PurposeExpresses the FLAG-tagged GeNL (Green Nanolantern) in mammalian cells.DepositorInsertGeNL Nanolantern
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCG-TPMV Hd32
Plasmid#163963PurposeExpresses TPMV H glycoprotein in mammalian cellsDepositorInsertTPMV H glycoprotein
UseTagsExpressionMammalianMutationAmino acids 2 to 33 at N terminus deletedPromoterAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Calnexin-HT8-mGold
Plasmid#183989PurposeExpresses fluorescent HaloTag8 fusion protein in mammalian cells with localization in the ER.DepositorInsertCalnexin (CANX Human)
UseTagsHaloTag8 (C61S, C262S), mGoldExpressionMammalianMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 p53 S15D
Plasmid#69005Purposemammalian expression of human p53 S15D mutantDepositorInsertp53 (TP53 Human)
UseTagsExpressionMammalianMutationS15DPromoterCMVAvailable sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDD315 (mTurquoise2^SEC^2xHA)
Plasmid#73343PurposemTurquoise2^SEC^2xHA vector with ccdB sites for cloning homology armsDepositorInsertmTurquoise2-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags2xHA and C. elegans codon-optimized mTurquoise2ExpressionWormMutationPromoterAvailable sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pCMV4a-SIRT6-H133Y-FLAG
Plasmid#102324PurposeHuman SIRT6 H133Y with FLAG tag for mammalian expressionDepositorInsertSIRT6 (SIRT6 Human)
UseTagsFLAGExpressionMammalianMutationchanged histidine 133 to tyrosinePromoterCMVAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133-tRNA2.0
Plasmid#158395PurposeGolden Gate entry vector to express the 3rd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
RFP-(SIM)1
Plasmid#127094PurposeBacterial expression plasmid for mCherry-(SIM)1 client to assess SUMO-SIM scaffolds.DepositorInsertmCherry - SUMO interacting motif (SIM) from PIASx (PIAS2 Human)
UseTagsmCherryExpressionBacterialMutationPromoterunknownAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcI-ts,ind+-(mtSSB delta20)
Plasmid#186280PurposeExpresses Human Mitochondrial SSB in E. coliDepositorInsertMitochondrial Single Stranded DNA Binding Protein (SSBP1 Human)
UseTagsExpressionBacterialMutationFirst 20 amino acids corresponding to probable mi…PromoterlambdaAvailable sinceJuly 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
ACTIN-SOX2-PGK Cre
Plasmid#59019PurposeBicistronic Lentivirus expressing sox2 and CreDepositorInsertSox2 (Sox2 Mouse)
UseLentiviralTagsExpressionMutationPromoterB-actinAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDuRCC-N
Plasmid#81194PurposeExpression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin ResistanceDepositorInsertsCas9
empty gRNA cassette
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease view depositor comments below and WT - cod…PromoterROX3 and SNP52pAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSL0374 (His-MBP-Cas8_Cas7_Cas6)
Plasmid#130639PurposeExpresses V. cholerae CAST Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on Cas8 for purification.DepositorInsertVchCas8, VchCas7, VchCas6
UseCRISPR; TransposonTagsHis10-MBP-TEVsite on Cas8ExpressionBacterialMutationPromoterT7Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ134-tRNA2.0
Plasmid#158396PurposeGolden Gate entry vector to express the 4th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-FUCCI
Plasmid#133363PurposePiggybac vector for shRNA-mediated knockdown, co-expressed with FUCCI cell cycle reporterDepositorInsertClover-Geminin-IRES-mKO2-Cdt1
UseTagsHA-tagged Clover-GemininExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
RFP-(SUMO)3
Plasmid#127150PurposeMammalian expression plasmid for mCherry-(SUMO)3 client to assess SUMO-SIM scaffolds.DepositorInsert3 copies of human SUMO1 separated by (GGS)4 (SUMO3 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP 3xMyc-STX17ΔNTD
Plasmid#89940PurposeExpresses syntaxin17 lacking N-terminal domain tagged with Myc in mammalian cellsDepositorInserthuman syntaxin17 lacking N-terminal domain (STX17 Human)
UseRetroviralTagsMycExpressionMutationdeleted amino acids 1-161PromoterAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
FKBP12-CB2x2-mTagBFP2-HAx2
Plasmid#207146PurposeTandem calcium-buffer with rapalog dimerizerDepositorInsertCalbindin-2 (CALB2 Human)
UseTagsFKBP12, HA, and mTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-MKRN1
Plasmid#78745PurposeTo overexpress MKRN1 in Mammalian CellsDepositorInsertMKRN1 (MKRN1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-CAAX
Plasmid#51567PurposeConstitutively express PM (Plasma membrane) localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
UseTagsCAAX and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable sinceAug. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
YFP Gamma 2
Plasmid#36102DepositorInsertG protein subunit Gamma 2 (GNG2 Bovine)
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-09 FL
Plasmid#47081DepositorInsertAL-09 (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pYPQ135-tRNA2.0
Plasmid#158397PurposeGolden Gate entry vector to express the 5th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pCAG-Cre
Plasmid#125574PurposeExpresses Cre under the CAG promoter in mammalian cells.DepositorInsertCre
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA1158_pPBCAG-MerCreMer-IZ
Plasmid#124184PurposepiggyBac-based expression of the tamoxifen-inducible CreDepositorInsertMerCreMer-IRES-Zeo
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
linker-mCitrine-PIF6-NAT
Plasmid#51576PurposeTag protein of interest with PIF6 in Saccharomyces cerevisiaeDepositorInsertPIF6 (PIL2 Mustard Weed)
UseTagslinker and mCitrineExpressionYeastMutation1-100aa of PIF6PromoternoneAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV/3' Box_(GLuc)_INT
Plasmid#68436PurposeTransient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.DepositorInsertINT construct_bearing three PP7 Stem-loops
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-OgNLuc_SV40:PuroR
Plasmid#183038PurposeExpresses the FLAG-tagged OgNLuc (Orange LumiFluor) in mammalian cells.DepositorInsertOgNLuc LumiFluor
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ145-ZmUbi-tRNA
Plasmid#158403PurposeGateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ135-tRNA2.0; assembly of 5 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterMaize ubiquitin 1Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ136-tRNA2.0
Plasmid#158398PurposeGolden Gate entry vector to express the 6th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-75-2
Plasmid#73036PurposeExpress an NT-3-BDNF hybrid neurotrophic factor, with reduced affinity to p75NTR, in mammalian cellsDepositorInsertp75-2
UseLentiviralTagsExpressionMammalianMutationMature Human NT-3 gene exchanged Aspartic 15 to a…PromoterAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
TPC2-D276K-G-GECO1.2
Plasmid#207142PurposeFusion of Ca2+ reporter and pore-dead lysosomal Ca2+-permeable channelDepositorInsertTPC2N2 (TPCN2 Human)
UseTagsG-GECO1.2ExpressionMammalianMutationChanged Aspartate 276 to LysinePromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
p1977 (pUC19-PTDH3-PTEF1)
Plasmid#78230PurposeExample plasmid containing a bidirectional promoter of pTDH3 and pTEF1DepositorInsertBidirectional promoters pTDH3-pTEF1
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMC_mNG2(11)_BSDminus_F0
Plasmid#184162PurposeDonor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 0, as well as a blasticidin resistance gene for selection of genomic integrants.DepositorInsertsmNG2(11)
bsd
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-FEN1DA
Plasmid#35028DepositorInsertFEN1DA (FEN1 Human)
UseAdenoviralTagsFlagExpressionMammalianMutationD181A--NUCLEASE DEAD POINT MUTANTPromoterCMVAvailable sinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBS ColIII alpha1
Plasmid#19187DepositorInsertProcollagen type III alpha 1 (COL3A1 Chicken)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-dpcoCas9-EV2.1
Plasmid#158411PurposeCRISPR-dpcoCas9 fused with EDLL activator for transcriptional activationDepositorInsertdpcoCas9-EDLL-T2A-MS2-VPR(VP64-p65-Rta)-NOS-ter
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Donor_eGP2AP_RC
Plasmid#133784PurposeLibrary reporter vector/integration vector. Contains library cloning sites MluI and SpeI upstream of a terminator and downstream of a BxB1 attB site. Library integration occurs through BxB1 site.DepositorTypeEmpty backboneUseTagseGFP-P2A-PuroExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-MKRN1_H307E
Plasmid#78756PurposeTo overexpress MKRN1_H307E in Mammalian CellDepositorInsertMKRN1 (MKRN1 Human)
UseTagsFLAGExpressionMammalianMutationH307EPromoterCMVAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only