Addgene

Showing: 4441 - 4460 of 8972 results

Results per page:

Sort results by:

pVT36b

Plasmid

#184437

Purpose

CRISPR/Cas9 plasmid, containing ScARS, IoURA3, iCas9, RPR1’-tRNA_Leu promoter, and sgRNA scaffold

Depositor

Huimin Zhao

Article

Tran et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003

Type

Empty backbone

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
YEp181-CUP1-His-Ubi

Plasmid

#99539

Purpose

Yeast episomal vector for expression of His-tagged ubiquitin; LEU2 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

UBI3(1-76) (RPL40A Budding Yeast)

Use

Tags

7xHis

Expression

Yeast

Mutation

Promoter

CUP1

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pYM70

Plasmid

#119922

Purpose

CaHygB plasmids that facilitate genetic manipulations in the fungal pathogen

Depositor

Brian Wong

Article

Basso LR et al Yeast. 2010 Dec;27(12):1039-48. doi: 10.

Insert

CaHygB

Use

Tags

CaHygB

Expression

Mutation

Promoter

TEF2

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHO-UAS-mScarlet-I

Plasmid

#183147

Purpose

Yeat two-hybrid fluorecent reporter for genomic integration

Depositor

Assaf Mosquna

Article

Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

Insert

mScarlet-I

Use

Tags

Expression

Mutation

Promoter

Available since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pC1-CMV-DAAO-NES

Plasmid

#141107

Purpose

Expresses DAAO with nuclear export signal in mammalian cells

Depositor

Vsevolod Belousov

Article

Bogdanova et al Curr Protoc Chem Biol. 2017 Jun 19;9(2):

Insert

DAAO-NES

Use

Tags

Nuclear export signal

Expression

Mammalian

Mutation

deleted DAAO amino acids 367-368

Promoter

CMV

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
bRA89

Plasmid

#100950

Purpose

Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HPH marker

Depositor

James Haber

Article

Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
JME4472

Plasmid

#129658

Purpose

URA3ex_CrisprCas9-yl_RFP: CAS9 vector with URA3ex marker for gRNA cloning using GoldenGate

Depositor

Tristan Rossignol

Article

Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
YEp181-CUP1-His-Smt3

Plasmid

#99536

Purpose

Yeast episomal vector for expression of His-tagged Smt3 (SUMO); LEU2 marker

Depositor

Helle Ulrich

Article

Ulrich et al Methods Mol Biol. 2009;497:81-103. doi:

Insert

SMT3 (SMT3 Budding Yeast)

Use

Tags

7xHis

Expression

Yeast

Mutation

Promoter

CUP1

Available since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pYZ714

Plasmid

#132955

Purpose

crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with dFnCas12a-Clr4 in Schizosaccharomyces pombe

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578

Insert

dFnCas12a

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS and nucleoplasmin NLS

Expression

Mutation

Promoter

adh1

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp128-P30-His-pol30(K164R)

Plasmid

#99544

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K164R; Leu2 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Use

Tags

6xHis

Expression

Yeast

Mutation

K164R

Promoter

POL30

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
wg186Fluc

Plasmid

#177436

Purpose

In vitro synthesis of an mRNA encoding Firefly luciferase with A[62] tail.

Depositor

Wendy Gilbert

Article

Rojas-Duran et al RNA. 2012 Dec;18(12):2299-305. doi: 10.1

Insert

Firefly luciferase

Use

Luciferase; For in vitro transcription

Tags

None

Expression

Mutation

Promoter

T7

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
FRP793_insul-(lexA-box)4-PminCYC1-Citrine-TCYC1

Plasmid

#58434

Purpose

Induces Citrine expression from a promoter containing 4 lexA boxes in yeast cells

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

CitrineA206K

Use

Tags

Expression

Yeast

Mutation

Promoter

insul-(lexA-box)4-PminCYC1

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV10-3xFLAG-prs-MCP1

Plasmid

#184341

Purpose

Express yeast MCP1 in Expi293F cells

Depositor

Karin Reinisch

Article

Adlakha et al J Cell Biol. 2022 Jun 6;221(5):e20220203

Insert

Yeast MCP1 (MCP1 Budding Yeast)

Use

Tags

3xFLAG-PreScission cleavage site

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pYR14 (XII-5:TDH3p:his6-mKate2-LipPks1-IDP1t)

Plasmid

#167357

Purpose

Integrative plasmid for cassette TDH3p:his6-mKate2-LipPks1-IDP1t

Depositor

Michael Jensen

Article

Romero-Suarez et al ACS Synth Biol. 2021 Feb 12. doi: 10.102

Insert

TDH3p:his6-mKate2-LipPks1-IDP1t

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB2225

Plasmid

#67553

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae XII-2 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEVY-GU

Plasmid

#51229

Purpose

bi-directional expression vector for yeast, galactose inducible, URA3 selection

Depositor

Charles Miller

Article

Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1,10

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEVY-GL

Plasmid

#51225

Purpose

bi-directional expression vector for yeast, galactose inducible, LEU2 selection

Depositor

Charles Miller

Article

Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1,10

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
Dhh1

Plasmid

#201412

Purpose

Bacterial protein expression of Dhh1

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Dhh1 (DHH1 Budding Yeast)

Use

Tags

MBP, TEV and TEV, 6xHis

Expression

Bacterial

Mutation

Promoter

tac

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pYR11 (XII-5:TDH3p:his6-mKate2-MBP-IDP1t)

Plasmid

#167356

Purpose

Integrative plasmid for cassette TDH3p:his6-mKate2-MBP-IDP1t

Depositor

Michael Jensen

Article

Romero-Suarez et al ACS Synth Biol. 2021 Feb 12. doi: 10.102

Insert

TDH3p:his6-mKate2-MBP-IDP1t

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pG/ER(G)

Plasmid

#108219

Purpose

Yeast expression vector for human estrogen receptor alpha

Depositor

Didier Picard

Article

Liu et al FEMS Microbiol Lett. 1998 Feb 15;159(2):

Insert

Human estrogen receptor a (ESR1 Human)

Use

Tags

Expression

Yeast

Mutation

Promoter

TDH3

Available since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge