pG/ER(G)
(Plasmid
#108219)
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PurposeYeast expression vector for human estrogen receptor alpha
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 108219 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepG-1
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Vector typeYeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHuman estrogen receptor a
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SpeciesH. sapiens (human)
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Entrez GeneESR1 (a.k.a. ER, ESR, ESRA, ESTRR, Era, NR3A1)
- Promoter TDH3
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pG/ER(G) was a gift from Didier Picard (Addgene plasmid # 108219 ; http://n2t.net/addgene:108219 ; RRID:Addgene_108219) -
For your References section:
Bioactive steroids as contaminants of the common carbon source galactose. Liu JW, Picard D. FEMS Microbiol Lett. 1998 Feb 15;159(2):167-71. 10.1111/j.1574-6968.1998.tb12856.x PubMed 9503609