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Plasmid#106010PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR22; Opto-GPR22; A12)DepositorInsertOpto-GPR22 (GPR22 Human, Bovine)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR26
Plasmid#106012PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR26; Opto-GPR26; B2)DepositorInsertOpto-GPR26 (GPR26 Human, Bovine)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_nmPKA-CαN-mPAGFP
Plasmid#114130PurposeMammalian expression of the G2A mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertthe N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP, with G2A mutation (Prkaca Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-Cα(S11D)-mPAGFP
Plasmid#114135PurposeMammalian expression of the S11D mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertthe mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11D mutation (Prkaca Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-Cα(S11A)-mPAGFP
Plasmid#114134PurposeMammalian expression of the S11A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertthe mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11A mutation (Prkaca Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN-mPAGFP
Plasmid#114114PurposeMammalian expression of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertthe N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP (Prkaca Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTE3314
Plasmid#107530PurposeExpresses human codon optimized FnCpf1(RVR mutant) in mammalian cells.DepositorInserthFnCpf1(RVR)
UseTags3xHA and NLSExpressionMammalianMutationN607R, K613V, N617RPromoterCMVAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTE3319
Plasmid#107533PurposeExpresses human codon optimized MbCpf1(RR mutant) in mammalian cells.DepositorInserthMbCpf1(RR)
UseTags3xHA and NLSExpressionMammalianMutationN576R, K637RPromoterCMVAvailable sinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTE3316
Plasmid#107532PurposeExpresses human codon optimized FnCpf1(RR mutant) in mammalian cells.DepositorInserthFnCpf1(RR)
UseTags3xHA and NLSExpressionMammalianMutationN607R, K671RPromoterCMVAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTE3317
Plasmid#107531PurposeExpresses human codon optimized MbCpf1(RVR mutant) in mammalian cells.DepositorInserthMbCpf1(RVR)
UseTags3xHA and NLSExpressionMammalianMutationN576R, K582V, N586RPromoterCMVAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
hASXL(1-115)-luciferase
Plasmid#106427PurposeExpresses human ASXL1 (amino acids 1-115) fused to the N-terminal of firefly luciferaseDepositorInsertaddition sex combs like 1 ASXL1 (ASXL1 Human)
UseTagsluciferaseExpressionMammalianMutationamino acids 1-115 fused to luciferasePromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Cx43-S279/282A
Plasmid#60712Purposemammalian expression of rat Cx43 S279/282A mutantDepositorInsertCx43 (Gja1 Rat)
UseTagsExpressionMammalianMutationS279/282APromoterCMVAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Cx43-S279/282D
Plasmid#60714Purposemammalian expression of rat Cx43 S279/282D mutantDepositorInsertCx43 (Gja1 Rat)
UseTagsExpressionMammalianMutationS279/282DPromoterCMVAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-HA/Bmal F423R/V435R/W427R
Plasmid#47351PurposePcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003DepositorInsertBMAL (Bmal1 Mouse)
UseTagsHaExpressionMammalianMutationF423R/V435R/W427RPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMV-mito-R-GECO1
Plasmid#46021PurposeRed intensiometric genetically encoded Ca2+-indicators for optical imagingDepositorInsertR-GECO1
UseTagsa duplex of the mitochondrial targeting signal of…ExpressionMammalianMutationSubstitutions relative to the mApple-derived anal…PromoterCMVAvailable sinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
CaMKAR
Plasmid#205315PurposeFluorescent Reporter for CaMKII ActivityDepositorInsertCaMKAR
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-jGCaMP8s
Plasmid#162371PurposeMammalian expression of ultrafast protein calcium sensorDepositorInsertjGCaMP8s
UseTags6xHisExpressionMammalianMutationS26M F286Y Q315HPromoterCMVAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-jGCaMP8m
Plasmid#162372PurposeMammalian expression of ultrafast protein calcium sensorDepositorInsertjGCaMP8m
UseTags6xHisExpressionMammalianMutationA25G F286YPromoterCMVAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-jGCaMP8f
Plasmid#162373PurposeMammalian expression of ultrafast protein calcium sensorDepositorInsertjGCaMP8f
UseTags6xHisExpressionMammalianMutationQ315LPromoterCMVAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Cas9-T2A-TdT
Plasmid#126424PurposeExpresses Cas9-T2A-TdT in mammalian cells. (pTBL209)DepositorInsertCas9-T2A-TdT (DNTT Streptococcus pyogenes, Human)
UseTagsExpressionMammalianMutationPromoterpCMVAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only