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Showing: 11051 - 11100 of 14292 results

  1. pTd-4-15-13-3

    Plasmid
    #63828
    Purpose
    This plasmid is designed to validate the biophysical model of translational coupling
    Depositor
    Howard Salis
    Article
    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71
    Insert
    CFP, mRFP1 and GFPmut3b
    Use
    Tags
    Expression
    Bacterial
    Mutation
    The intergenic distance between each gene is -4 n…
    Promoter
    J23100
    Available since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  2. pTd-4-15-13-4

    Plasmid
    #63829
    Purpose
    This plasmid is designed to validate the biophysical model of translational coupling
    Depositor
    Howard Salis
    Article
    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71
    Insert
    CFP, mRFP1 and GFPmut3b
    Use
    Tags
    Expression
    Bacterial
    Mutation
    The intergenic distance between each gene is -4 n…
    Promoter
    J23100
    Available since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  3. pTd-4-15-13-5

    Plasmid
    #63830
    Purpose
    This plasmid is designed to validate the biophysical model of translational coupling
    Depositor
    Howard Salis
    Article
    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71
    Insert
    CFP, mRFP1 and GFPmut3b
    Use
    Tags
    Expression
    Bacterial
    Mutation
    The intergenic distance between each gene is -4 n…
    Promoter
    J23100
    Available since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  4. pTd-4-15-13-6

    Plasmid
    #63831
    Purpose
    This plasmid is designed to validate the biophysical model of translational coupling
    Depositor
    Howard Salis
    Article
    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71
    Insert
    CFP, mRFP1 and GFPmut3b
    Use
    Tags
    Expression
    Bacterial
    Mutation
    The intergenic distance between each gene is -4 n…
    Promoter
    J23100
    Available since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  5. pTd-4-15-13-7

    Plasmid
    #63832
    Purpose
    This plasmid is designed to validate the biophysical model of translational coupling
    Depositor
    Howard Salis
    Article
    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71
    Insert
    CFP, mRFP1 and GFPmut3b
    Use
    Tags
    Expression
    Bacterial
    Mutation
    The intergenic distance between each gene is -4 n…
    Promoter
    J23100
    Available since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  6. pTd-4-15-13-8

    Plasmid
    #63833
    Purpose
    This plasmid is designed to validate the biophysical model of translational coupling
    Depositor
    Howard Salis
    Article
    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71
    Insert
    CFP, mRFP1 and GFPmut3b
    Use
    Tags
    Expression
    Bacterial
    Mutation
    The intergenic distance between each gene is -4 n…
    Promoter
    J23100
    Available since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  7. pTd-4-15-13-9

    Plasmid
    #63834
    Purpose
    This plasmid is designed to validate the biophysical model of translational coupling
    Depositor
    Howard Salis
    Article
    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71
    Insert
    CFP, mRFP1 and GFPmut3b
    Use
    Tags
    Expression
    Bacterial
    Mutation
    The intergenic distance between each gene is -4 n…
    Promoter
    J23100
    Available since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  8. pTd-4-15-13-11

    Plasmid
    #63836
    Purpose
    This plasmid is designed to validate the biophysical model of translational coupling
    Depositor
    Howard Salis
    Article
    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71
    Insert
    CFP, mRFP1 and GFPmut3b
    Use
    Tags
    Expression
    Bacterial
    Mutation
    The intergenic distance between each gene is -4 n…
    Promoter
    J23100
    Available since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  9. pU6_(GLuc)_INT[S1Apt]

    Plasmid
    #68425
    Purpose
    Transient expression in mammalian cells of an "INT" construct_bearing the "S1" streptavidin aptamer, targeting the GLuc reporter. U6 promoter
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    INT construct with S1 streptavidin aptamer
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68425-plasmid-map-sequence-id-120203

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  10. pmalc4x_G252R_TEV

    Plasmid
    #47704
    Purpose
    Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation G252R
    Depositor
    Raquel Lieberman
    Article
    Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.
    Insert
    Olfactomedin-like domain with G252R mutation (MYOC Human)
    Use
    Tags
    MBP
    Expression
    Bacterial
    Mutation
    changed Glycine 252 Arginine
    Promoter
    Ptac
    Available since
    Sept. 9, 2013
    Availability
    Academic Institutions and Nonprofits only
    47704-plasmid-map-sequence-id-68124

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  11. pmalc4x_K398R_TEV

    Plasmid
    #47701
    Purpose
    Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation K398R
    Depositor
    Raquel Lieberman
    Article
    Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.
    Insert
    Olfactomedin-like domain with K398R mutation (MYOC Human)
    Use
    Tags
    MBP
    Expression
    Bacterial
    Mutation
    changed Lysine 398 to Arginine
    Promoter
    Ptac
    Available since
    Sept. 9, 2013
    Availability
    Academic Institutions and Nonprofits only
    47701-plasmid-map-sequence-id-68116

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  21. pDD2013

    Plasmid
    #101364
    Purpose
    Name: pCBG68-K14ΔTATA. K14 TATA box mutation in pCBG68-LANApi (pDD2002).
    Depositor
    Dirk Dittmer
    Article
    Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1
    Insert
    K14 TATA box mutation in pCBG68-LANApi (pDD2002).
    Use
    Unspecified
    Tags
    Expression
    Mutation
    K14 TATA box mutation in pCBG68-LANApi (pDD2002).
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    101364-plasmid-map-sequence-id-194070

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  22. pDD2026

    Plasmid
    #101369
    Purpose
    Name: pCBG68-LANApiΔTATA. LANApi TATA box mutation in pCBG68-LANApi (pDD2002).
    Depositor
    Dirk Dittmer
    Article
    Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1
    Insert
    LANApi TATA box mutation in pCBG68-LANApi (pDD2002).
    Use
    Luciferase
    Tags
    Expression
    Mutation
    LANApi TATA box mutation in pCBG68-LANApi (pDD200…
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    101369-plasmid-map-sequence-id-194097

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  23. pDD2027

    Plasmid
    #101370
    Purpose
    Name: pCBR-LANApiΔTATA. LANApi TATA box mutation in pCBR-K14 (pDD2005).
    Depositor
    Dirk Dittmer
    Article
    Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1
    Insert
    LANApi TATA box mutation in pCBR-K14 (pDD2005).
    Use
    Luciferase
    Tags
    Expression
    Mutation
    LANApi TATA box mutation in pCBR-K14 (pDD2005).
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    101370-plasmid-map-sequence-id-194098

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  27. pWPT-/GCCACC-mEGFP-IRES-mCherry

    Plasmid
    #49235
    Purpose
    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Clifford Wang
    Article
    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110
    Inserts
    mEGFP
    mCherry
    Use
    Lentiviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-short
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49235-plasmid-map-sequence-id-376544

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  29. PB-SAM-Dest

    Plasmid
    #102563
    Purpose
    Piggybac transposon DEST vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex. It is used for insertion of multiple U6-sgRNA expression cassettes.
    Depositor
    Ying Liu
    Article
    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    N/A
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    102563-plasmid-map-sequence-id-193893

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  30. pYPQ131-tRNA2.0

    Plasmid
    #158393
    Purpose
    Golden Gate entry vector to express the 1st gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Without promoter
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    158393-plasmid-map-sequence-id-330365

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  37. pYPQ134B2.0

    Plasmid
    #167158
    Purpose
    Golden Gate entry vector to express the 4th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    AtU3
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    167158-plasmid-map-sequence-id-331276

    Enlarge

  47. pSR657

    Plasmid
    #65214
    Purpose
    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-2 cap gene in Sf9 insect cells
    Depositor
    Robert Kotin
    Article
    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:
    Inserts
    modified AAV-2 rep78
    modified AAV-2 cap
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    p10 and polyhedrin
    Available since
    June 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    65214-plasmid-map-sequence-id-116341

    Enlarge

  48. pSR646

    Plasmid
    #65215
    Purpose
    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-6 cap gene in Sf9 insect cells
    Depositor
    Robert Kotin
    Article
    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:
    Inserts
    modified AAV-2 rep78
    modified AAV-6 cap
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    P10 and polyhedrin
    Available since
    May 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    65215-plasmid-map-sequence-id-112598

    Enlarge

  49. pSR660

    Plasmid
    #65216
    Purpose
    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-8 cap gene in Sf9 insect cells
    Depositor
    Robert Kotin
    Article
    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:
    Inserts
    modified AAV-2 rep78
    modified AAV-8 cap
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    P10 and polyhedrin
    Available since
    June 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    65216-plasmid-map-sequence-id-115626

    Enlarge

  50. pU6_(GLuc)_INT[3xPP7_SL]

    Plasmid
    #68424
    Purpose
    Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. U6 promoter
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    INT construct bearing three PP7 Stem-loops
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68424-plasmid-map-sequence-id-125555

    Enlarge

Showing: 11051 - 11100 of 14292 results