Addgene

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p2lox-cAMPr

Plasmid

#99143

Purpose

Expresses cAMPr in mammalian cells

Depositor

Justin Blau

Article

Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/

Insert

cAMPr

Use

Cre/Lox

Tags

cpGFP

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pVAX1/mTyr-Cre

Plasmid

#99249

Purpose

Expresses Cre recombinase under tyrosinase promoter

Depositor

Christian Blank

Article

Deken et al Int J Mol Sci. 2016 Dec 20;17(12). pii:

Inserts

Cre recombinase
mTyr enh/prom

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pAdCMV/V5-DEST-STAT3-3xFlag

Plasmid

#99260

Purpose

Adenoviral vector encoding Flag-tagged murine STAT3

Depositor

Alejandro Adam

Article

Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

Insert

Signal transducer and activator of transcription 3 (Stat3 Mouse)

Use

Adenoviral

Tags

3x-Flag

Expression

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pAdCMV/V5-DEST-Y705F-STAT3-3xFlag

Plasmid

#99261

Purpose

Adenoviral vector encoding Flag-tagged murine STAT3 (Y705F)

Depositor

Alejandro Adam

Article

Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

Insert

Signal transducer and activator of transcription 3 - Y705F (Stat3 Mouse)

Use

Adenoviral

Tags

3x-Flag

Expression

Mutation

Y705F (contains a silent mutation: nucleotide 271…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pAdCMV/V5-DEST-S727A-STAT3-3xFlag

Plasmid

#99262

Purpose

Adenoviral vector encoding Flag-tagged murine STAT3 (S727A)

Depositor

Alejandro Adam

Article

Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

Insert

Signal transducer and activator of transcription 3 - S727A (Stat3 Mouse)

Use

Adenoviral

Tags

3x-Flag

Expression

Mutation

S727A

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pAdCMV/V5-DEST-S727D-STAT3-3xFlag

Plasmid

#99263

Purpose

Adenoviral vector encoding Flag-tagged murine STAT3 (S727D)

Depositor

Alejandro Adam

Article

Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

Insert

Signal transducer and activator of transcription 3 - S727D (Stat3 Mouse)

Use

Adenoviral

Tags

3x-Flag

Expression

Mutation

S727D

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pAdCMV/V5-DEST-STAT3C-3xFlag

Plasmid

#99264

Purpose

Adenoviral vector encoding Flag-tagged murine STAT3C (constitutive)

Depositor

Alejandro Adam

Article

Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

Insert

Signal transducer and activator of transcription 3 - constitutive dimer (Stat3 Mouse)

Use

Adenoviral

Tags

3x-Flag

Expression

Mutation

A662C N664C

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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hEF1α-H2B-mOrange2-IRES-EGFP PGK-Puromycin

Plasmid

#99277

Purpose

Dual fluorescent reporter - constitutive expression of mOrange2 (549/565) localized in the nucleus, EGFP (488/507) untargeted and visualized in the cytoplasm, and Puromycin resistance

Depositor

Jordan Miller

Article

Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/

Inserts

H2B-mOrange2-IRES-EGFP
Puro

Use

Lentiviral

Tags

EGFP, H2B, IRES, PGK, and mOrange2

Expression

Mutation

Promoter

EF1 alpha-human and PGK-mouse

Availability

Academic Institutions and Nonprofits only

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hEF1α-H2B-mVenus-IRES-mCherry PGK-Puromycin

Plasmid

#99278

Purpose

Dual fluorescent reporter - constitutive expression of mVenus (515/528) localized in the nucleus, mCherry (587/610) untargeted and visualized in the cytoplasm, and Puromycin resistance

Depositor

Jordan Miller

Article

Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/

Inserts

H2B-mVenus-IRES-mCherry
Puro

Use

Lentiviral

Tags

H2B, IRES, PGK, mCherry, and mVenus

Expression

Mutation

Promoter

EF1 alpha-human and PGK-mouse

Availability

Academic Institutions and Nonprofits only

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pCW57.1-mDux-CA

Plasmid

#99284

Purpose

All-in-one lentivector with tet-inducible mouse Dux (codon sequences altered)

Depositor

Stephen Tapscott

Article

Whiddon et al Nat Genet. 2017 Jun;49(6):935-940. doi:

Insert

Dux-CA (mouse; codon altered) (Dux Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Availability

Academic Institutions and Nonprofits only

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pET-ZZ(1700-1751)

Plasmid

#99334

Purpose

Bacterial expression of mouse CBP ZZ domain

Depositor

Peter Wright

Article

Legge et al J Mol Biol. 2004 Oct 29;343(4):1081-93.

Insert

CBP ZZ domain (Crebbp Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pET-NCBD + ACTR

Plasmid

#99335

Purpose

Bacterial coexpression of CBP NCBD and NCoA3 binding domains

Depositor

Peter Wright

Article

Demarest et al Nature. 2002 Jan 31;415(6871):549-53.

Inserts

CBP NCBD (2059-2117) (CREBBP Mouse)
SRC3 (1023-1093) (NCOA3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pET-KIX (586-672)

Plasmid

#99336

Purpose

Bacterial expression of mouse CBP KIX domain

Depositor

Peter Wright

Article

Radhakrishnan et al J Mol Biol. 1999 Apr 16;287(5):859-65.

Insert

CBP KIX (586-672) (Crebbp Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pET-CH2 (1192-1318)

Plasmid

#99338

Purpose

Bacterial expression of mouse CBP CH2 domain

Depositor

Peter Wright

Article

Park et al FEBS Lett. 2013 Aug 19;587(16):2506-11.

Insert

CBP CH2 (1192-1318) (Crebbp Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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GST-BRDCH2 (1079-1317)

Plasmid

#99339

Purpose

Bacterial expression of mouse CBP BRDCH2 domains as a GST fusion

Depositor

Peter Wright

Article

Park et al FEBS Lett. 2013 Aug 19;587(16):2506-11.

Insert

mouse CBP BRDCH2 (1079-1317) (Crebbp Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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His6tev CBP HAT (1324-1700)

Plasmid

#99340

Purpose

bacterial expression of His tagged mouse CBP HAT domain (1324-1700)

Depositor

Peter Wright

Article

Park et al Proc Natl Acad Sci U S A. 2017 Jun 19. p

Insert

mouse CBP HAT domain (1324-1700) (Crebbp Mouse)

Use

Tags

His6tev

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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His6tev CBP BCHZ (1079-1751)

Plasmid

#99341

Purpose

Bacterial expression of His tagged mouse CBP HAT core Brd-ZZ (1079-1751)

Depositor

Peter Wright

Article

Park et al Proc Natl Acad Sci U S A. 2017 Jun 19. p

Insert

mouse CBP BRD-ZZ domains (1079-1751) (Crebbp Mouse)

Use

Tags

His6tev

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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HisGb1-STAT1(710-750) + CBP TAZ2 (1764-1855)

Plasmid

#99342

Purpose

Bacterial expression of mouse CBP TAZ2 domain by coexpression with HisGB1 tagged STAT1 TAD

Depositor

Peter Wright

Article

Wojciak et al EMBO J. 2009 Apr 8;28(7):948-58. doi: 10

Inserts

STAT1 (710-750) (STAT1 Human)
CBP (1764-1855)

Use

Tags

His6GB1

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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HisGB1-Hif1a(776-826) + CBP TAZ1(340-439)

Plasmid

#99343

Purpose

Bacterial expression of mouse CBP TAZ1 domain as coexpression with Hif1a TAD

Depositor

Peter Wright

Article

Sugase et al Protein Expr Purif. 2008 Feb;57(2):108-1

Inserts

Hif1a (776-826) (HIF1A Human)
CBP (340-439)

Use

Tags

His6GB1

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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lenti-EF1a-dCas9-VPR-Puro

Plasmid

#99373

Purpose

3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)

Depositor

Kristen Brennand

Article

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Insert

(Sp)dCas9-VPR-P2A-Puro

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Availability

Academic Institutions and Nonprofits only

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