pBSFI-myr-akt-S473A
(Plasmid
#49190)
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PurposecDNA of Akt1 with myristyolation signal and point mutation from S to A at position 473 in cloning vector
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 49190 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBSFI
- Backbone size w/o insert (bp) 3000
- Total vector size (bp) 4500
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Modifications to backbonepBSFI is based on pBluescript SK, with a different MCS inserted between SacI and KpnI
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Vector typeAdaptor Plasmid
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAkt1
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Alt nameAkt
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SpeciesM. musculus (mouse)
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Insert Size (bp)1500
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MutationS473A
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GenBank IDNM_009652.3
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Entrez GeneAkt1 (a.k.a. Akt, LTR-akt, PKB, PKB/Akt, PKBalpha, Rac)
- Promoter T7
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Tags
/ Fusion Proteins
- myristolation signal (N terminal on insert)
- HA tag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (destroyed during cloning)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBSFI-myr-akt-S473A was a gift from Peter Vogt (Addgene plasmid # 49190 ; http://n2t.net/addgene:49190 ; RRID:Addgene_49190) -
For your References section:
The akt kinase: molecular determinants of oncogenicity. Aoki M, Batista O, Bellacosa A, Tsichlis P, Vogt PK. Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14950-5. 10.1073/pnas.95.25.14950 PubMed 9843996