Addgene

Showing: 421 - 440 of 5192 results

Results per page:

Sort results by:

pCAP03-acc(3)IV

Plasmid

#69862

Purpose

pCAP03-acc(3)IV is a upgraded version of the pCAP01(Plasmid #59981)

Depositor

Bradley Moore

Article

Tang et al ACS Chem Biol. 2015 Oct 21.

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Nov. 23, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMA-SpCas9-g6

Plasmid

#80789

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 6/16/26 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
SpCas9-CBE6d

Plasmid

#215822

Purpose

Express CBE6d (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6d-SpCas9-2xUGI (cas9 )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-FlpeERT2

Plasmid

#14756

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

Flpe-ERT2 fusion protein

Use

Flp/frt

Tags

ERT2

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS-4591NES

Plasmid

#138377

Purpose

Booster-ROCK, a Red-Shifted Genetically Encoded FRET Biosensor for ROCK activity.

Depositor

Michiyuki Matsuda

Article

Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

Insert

Booster-ROCK

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-YTB

Plasmid

#26721

Depositor

Edward Callaway

Article

Marshel et al Neuron. 2010 Aug 26. 67(4):562-74.

Insert

venusYFP, TVA950, Rabies Glycoprotein (RABVgp4, CD320 Chicken, Rabies virus (strain SAD B19))

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-iRFP720

Plasmid

#89687

Purpose

pCAG promoter driving iRFP720

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

iRFP720

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
SpCas9-CBE6c

Plasmid

#215821

Purpose

Express CBE6c (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6c-SpCas9-2xUGI (cas9 )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCarCBADE_ProB(I69E)A

Plasmid

#117756

Purpose

ProB(I69E)A and codon optimized CarCBADE enzymes (in that order) in pBbaA5k backbone

Depositor

Greg Bokinsky

Article

Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

Insert

Glutamate kinase (ProA), mutant pyrroline-5-carboxylate synthase (ProBI69E) from E. coli and codon optimised CarCBADE operon

Use

Tags

Expression

Bacterial

Mutation

Promoter

PLac(UV5)

Available since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
eSpCas9(1.1)

Plasmid

#138555

Purpose

Expresses human codon-optimized enhanced specificity SpCas9 and blasticidin resistance: EFS promoter-eSpCas9(1.1)-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

eSpCas9(1.1)

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

K848A, K1003A, R1060A

Promoter

EFS

Available since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pX459_gRNA-AAVS1_hspCas9

Plasmid

#193309

Purpose

Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)

Depositor

Simon Elsaesser

Article

Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

Insert

Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

not applicable

Promoter

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP777_SnoopCatcher-p65HSF1-CO

Plasmid

#211770

Purpose

SnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional activator p65HSF1, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

SnoopCatcher-p65HSF1

Use

Tags

2xOLLAS

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect p65HSF…

Promoter

pEF1a and pSV40

Available since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-CyRFP1

Plasmid

#84356

Purpose

Expresses CyRFP1 fluorescent protein in an empty backbone, under CAG promoter

Depositor

Ryohei Yasuda

Article

Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

CyRFP1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-Bmpr2

Plasmid

#163403

Purpose

Expresses BMPR-2 in mammalican cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-B19L

Plasmid

#59922

Purpose

Expression vector for Rabies virus SAD B19 RNA dependent RNA polymerase gene

Depositor

Ian Wickersham

Insert

Rabies virus RNA dependent RNA polymerase

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG mitoYFP

Plasmid

#168508

Purpose

Expression of mito-EYFP (mitochondrial matrix targeted EYFP) for visualization of mitochondrial dynamics

Depositor

Franck Polleux

Article

Lewis TL et al Curr Biol. 2016 Oct 10;26(19):2602-2608.

Insert

mitoYFP

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-hCas9

Plasmid

#51142

Purpose

Expresses hCas9 under the CAG promoter for CRISPR

Depositor

Izuho Hatada

Article

Horii et al Int J Mol Sci. 2013 Sep 30;14(10):19774-

Insert

hCas9

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only
eSpCas9-EF-5Pou5f1

Plasmid

#174872

Purpose

CRISPR vector for generating Pou5f1 STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

sgRNA for mouse Pou5f1 (Pou5f1 Synthetic)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS-Bxb1o_nlsFlag

Plasmid

#119901

Purpose

Expresses Bxb1 integrase in mammalian cells

Depositor

Orion Weiner

Article

Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

Insert

Mammalian codon optimized Bxb1 integrase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCALNL-DsRed

Plasmid

#13769

Purpose

Cre recombinase-dependent expression of DsRed, i.e. DsRed is only expressed in the presence of Cre due to a transcriptional stop in between the loxP sites.

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

DsRed2

Use

Cre/Lox; Flp/frt

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge