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Addgene

pCAGGS-4591NES
(Plasmid #138377)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138377 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCAGGS
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 7348
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Booster-ROCK
  • Species
    S. cerevisiae (budding yeast), Synthetic
  • Insert Size (bp)
    2600
  • Promoter CAG

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Sequences are based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence). Sequence provided by depositing laboratory may be theoretical/predicted or based on Sanger/NGS sequencing results. Discrepancies between sequencing results obtained by Addgene and the original sequence provided by the depositor may be present.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGGS-4591NES was a gift from Michiyuki Matsuda (Addgene plasmid # 138377 ; http://n2t.net/addgene:138377 ; RRID:Addgene_138377)
  • For your References section:

    Booster, a Red-Shifted Genetically Encoded Forster Resonance Energy Transfer (FRET) Biosensor Compatible with Cyan Fluorescent Protein/Yellow Fluorescent Protein-Based FRET Biosensors and Blue Light-Responsive Optogenetic Tools. Watabe T, Terai K, Sumiyama K, Matsuda M. ACS Sens. 2020 Feb 26. doi: 10.1021/acssensors.9b01941. 10.1021/acssensors.9b01941 PubMed 32101394