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Plasmid#111877PurposeThis plasmid expresses Venus fluorescent protein fused to endogenous Histone H2B (H2B) gene of Capsaspora (CAOG_01818). It can be used to transfect Capsaspora cells and visualize nucleus in vivo.DepositorInsertCapsaspora Histone H2B (CoH2B) fused to Venus
UseCapsaspora owczarzakiTagsVenusExpressionMutationPromoterElongation Factor 1 alpha (EF1a) from CapsasporaAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
ss-ED-mCherry-GPI
Plasmid#163680PurposeExprsses the GPI-anchored luminal domain of ST6GAL1 with a mCherry tag in mammalian cellsDepositorInsertThe luminal domain of ST6 beta-galactoside alpha-2,6-sialyltransferase 1 with N-terminally tagged signal sequences (ST6GAL1 Human)
UseTagsGPI anchor, mCherry, and signal sequencesExpressionMammalianMutationThis chimera contains the ecto-domain of ST. Its …PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-neo-C11orf51-3xFlag (2765)
Plasmid#39859DepositorInsertC11orf51 (ANAPC15 Human)
UseTags3xFlagExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
DNMT2Δ191-237
Plasmid#198382PurposeBacterial expression plasmid for human DNMT2 deletion mutant; for binding assaysDepositorInsertDNMT2 (TRDMT1 Human)
UseTagsHis-tagExpressionBacterialMutationdeleted amino acids 191-237PromoterT7Available sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcdna3.1_mouseST6galI
Plasmid#178435Purposemurine St6gal1 expression plasmidDepositorInsertSt6gal1 (St6gal1 Mouse)
UseTags6xHIS-TEV Cleavage SiteExpressionMammalianMutationCoding sequnce for amino acids 85-403PromoterCMVAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
BMP10 N67Q N131Q D4G (S2-2)
Plasmid#184622Purposefor transfection into S2 cells and expression of BMP10 proteinDepositorInsertBMP10 (BMP10 Human)
UseTags8x His - streptavidin binding peptideExpressionInsectMutationN67Q, N131Q, ARIRR(312-316) replaced with GPromoterAvailable sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
TMD20
Plasmid#163675PurposeExpresses a ST6GAL1 mutant with 20 amino acids in its TMD in mammalian cellsDepositorInsertpartial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
UseTagsGFPExpressionMammalianMutationFor this partial length ST chimera, two restricti…PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
TMD21
Plasmid#163676PurposeExpresses a ST6GAL1 mutant with 21 amino acids in its TMD in mammalian cellsDepositorInsertpartial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
UseTagsGFPExpressionMammalianMutationFor this partial length ST chimera, two restricti…PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
TMD22
Plasmid#163677PurposeExpresses a ST6GAL1 mutant with 22 amino acids in its TMD in mammalian cellsDepositorInsertpartial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
UseTagsGFPExpressionMammalianMutationFor this partial length ST chimera, two restricti…PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
TMD24
Plasmid#163678PurposeExpresses a ST6GAL1 mutant with 24 amino acids in its TMD in mammalian cellsDepositorInsertpartial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
UseTagsGFPExpressionMammalianMutationFor this partial length ST chimera, two restricti…PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
NSS-GFP
Plasmid#163661PurposeExpresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail from TNF and TMD and luminal domain from ST6GAL1 in mammalian cellsDepositorInsertUseTagsGFPExpressionMammalianMutationThis chimera contains the cytosolic tail from TNF…PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-MAVS-mTA3
Plasmid#171001PurposeHiLITR protease with MAVS [Y536delinsFIV] C-terminal tmd targeting information (Mitochondria and ER insertion)DepositorInsertEGFP-uTEV1-MAVS(tmd)[Y536delinsFIV]
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationMAVS(tmd)[Y536delinsFIV]PromoterpTRE-tight (TetOn)Available sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-MAVS-mTA5
Plasmid#171003PurposeHiLITR protease with MAVS [Y536delinsFIVLI, R537A] C-terminal tmd targeting information (Mitochondria and ER insertion)DepositorInsertEGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI, R537A]
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationMAVS(tmd)[Y536delinsFIVLI, R537A]PromoterpTRE-tight (TetOn)Available sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSUMO FAPP2-GLTPH
Plasmid#170739PurposeThe plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.DepositorInsertPLEKHA8-GLTPH (PLEKHA8 Human)
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSUMO ACD11
Plasmid#170738PurposeThe plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.DepositorInsertacd11 (ACD11 Mustard Weed)
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
Clim Actin MCH Puro (CAMP)
Plasmid#104446PurposeExpresses mCherry under endogenous actin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope. Puromycin resistant.DepositorInsertmCherry
UseExpression in corallochytrium (single-celled euka…TagsExpressionMutationPromoteractinAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDRf1-4CL5-AtSDT (JBEI-11535)
Plasmid#87934PurposeCo-expression in S.cerevisiae of 4CL5 and AtSDTDepositorUseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG RpH-LAMP1-3xFLAG
Plasmid#163018Purposeexpresses ratiometric sensor of lysosomal lumenal pHDepositorInsertRpH-LAMP1-3xFLAG (Lamp1 Mouse)
UseTags3XFLAGExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAcGP67A-TfR
Plasmid#12130DepositorInserttransferrin receptor (TFRC Human)
UseTags6X-His tag, Factor Xa cleavage site, and leader p…ExpressionInsectMutationThe portion of the human TfR gene encoding residu…PromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDONOR223 RpH-LAMP1-3xFLAG
Plasmid#163019Purposegateway donor vector encoding ratiometric sensor of lysosomal lumenal pHDepositorInsertRpH-LAMP1-3xFLAG (Lamp1 Mouse)
UseGateway systemTags3XFLAGExpressionMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only