DNMT2Δ191-237
(Plasmid #198382)

• Purpose: Bacterial expression plasmid for human DNMT2 deletion mutant; for binding assays
• Depositing Lab: Tanja Schirmeister
• Publication: Schwickert et al ACS Med Chem Lett. 2023 May 9;14(6):777-787. doi: 10.1021/acsmedchemlett.3c00062. eCollection 2023 Jun 8.

Backbone

• Vector backbone: pET-28a(+)
• Total vector size (bp): 6328
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Use E. coli Rosetta 2 (DE3) pLysS cells for expression
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: DNMT2
• Alt name: TRDMT1
• Alt name: tRNA aspartic acid methyltransferase 1
• Species: H. sapiens (human)
• Mutation: deleted amino acids 191-237
• Entrez Gene: TRDMT1 (a.k.a. DMNT2, DNMT2, MHSAIIP, PUMET, RNMT1)
• Promoter: T7
• Tag / Fusion Protein:
  • His-tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry