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MYMH 395

Plasmid

#138062

Purpose

Encodes Anderson promoter variant using mRFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23109- pSEVA-331Bb- RFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 394

Plasmid

#138061

Purpose

Encodes Anderson promoter variant using mRFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23108- pSEVA-331Bb- RFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 393

Plasmid

#138060

Purpose

Encodes Anderson promoter variant using mRFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23105- pSEVA-331Bb- RFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 75

Plasmid

#138052

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-WTRBS-GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pSB1C3-ysg(NT)

Plasmid

#138263

Purpose

Subcloning and expression of non-targeting sgRNA for use in yeast dual reporter system.

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

Nontargeting sgRNA

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

SNR52

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pMM765

Plasmid

#133602

Purpose

Part Plasmid for dCas9 NLS Stop, Part 3(coding sequence)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

dCas9 NLS Stop

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pMM766

Plasmid

#133603

Purpose

Part Plasmid for dCas9 NLS Stop, Part 3b(coding sequence)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

dCas9 NLS Stop

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAL- S. pombe TSC1 (1-431)

Plasmid

#132616

Purpose

expresses S. pombe TSC1 (1-431) in E. coli

Depositor

Wenqing Xu

Article

Sun et al Nat Commun. 2013;4:2135. doi: 10.1038/nc

Insert

TSC1 (1-431)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pLY86

Plasmid

#130949

Purpose

Engineered sgRNA-LEA2-WTmis2 generator circuit including promoter Plux2, 2 BoxB aptamers and 2 mis-matches in sgRNA scaffold

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEA2-WTmis2

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Plux2

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pSTAP-seq_fly_spike-in

Plasmid

#125151

Purpose

Vector to measure CP candidates (spike-in) expression by determining the abundance of transcripts originating from the candidate by a D.pse enhancer in fly cells

Depositor

Alexander Stark

Article

Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

Insert

D. pseudoobscura peak #47 enhancer

Use

Stap-seq screening vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pWS1859

Plasmid

#123065

Purpose

Positive regulator of Ste20

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Ste50 [3]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pWS511

Plasmid

#123063

Purpose

Negative regulator of Ste12

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Dig2 [3]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS510

Plasmid

#123062

Purpose

Negative regulator of Ste12

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Dig1 [3]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS509

Plasmid

#123061

Purpose

Negative regulator of Fus3

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Msg5 [3]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS081

Plasmid

#123060

Purpose

Regulator of G protein signalling

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Sst2 [3]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pWS930

Plasmid

#123054

Purpose

Beta-estradiol inducible DNA binding domain

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Z3E [3a]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pWS625

Plasmid

#123046

Purpose

Adenosine GPCR

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

A2BR [3]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS626

Plasmid

#123045

Purpose

P-factor GPCR

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Mam2 [3]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS495

Plasmid

#123043

Purpose

Core promoter

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pRNR2m [2b]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS497

Plasmid

#123042

Purpose

Core promoter

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pCUP1m [2b]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge