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Plasmid#72970PurposeLevel 0 - SJM906 promoter (5' CTAT/3' GTAC fusion)DepositorInsertSJM906 promoter
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-SJM908
Plasmid#72971PurposeLevel 0 - SJM908 promoter (5' CTAT/3' GTAC fusion)DepositorInsertSJM908 promoter
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-SJM910
Plasmid#72972PurposeLevel 0 - SJM910 promoter (5' CTAT/3' GTAC fusion)DepositorInsertSJM910 promoter
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-SJM912
Plasmid#72974PurposeLevel 0 - SJM912 promoter (5' CTAT/3' GTAC fusion)DepositorInsertSJM912 promoter
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-SJM914
Plasmid#72975PurposeLevel 0 - SJM914 promoter (5' CTAT/3' GTAC fusion)DepositorInsertSJM914 promoter
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-SJM915
Plasmid#72976PurposeLevel 0 - SJM915 promoter (5' CTAT/3' GTAC fusion)DepositorInsertSJM915 promoter
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-T7
Plasmid#72977PurposeT7 promoter (5' CTAT/3' GTAC fusion)DepositorInsertT7 promoter
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP_BBa_B0034
Plasmid#72980PurposeBba_B0034 iGEM RBS (5' GTAC/3' CATA fusion)DepositorInsertBba_B0034 RBS
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-Tag_linker
Plasmid#72982PurposeN-terminal linker with pET-RBS (5' GTAC/3' TAAA fusion)DepositorInsertN-terminal linker with pET-RBS
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-HexHis
Plasmid#72983PurposeHexahistidine N-terminal tag (5' TAAA/3' CATA fusion)DepositorInsertPolyhistidine (HHHHHH) N-terminal tag
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-ORF-eGFP
Plasmid#72960PurposeLevel 0 Entry vector" ORF - eGFP "DepositorInsertEnhanced green flourescent protein (eGFP)
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
AEB-T4-2
Plasmid#63855PurposeThis pFTV1 vector contains a thyroxine binding riboswitch, controlling the translation initaition rate of luciferase gene.DepositorInsertT4-2 thyroxine riboswitch
UseSynthetic BiologyTagsExpressionMutationPromotertacAvailable sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSTARR-seq_fly-x16
Plasmid#71505PurposeflySTARR-seq screening vector with x16 core promoterDepositorTypeEmpty backboneUseFlystarr-seq screening vectorTagssgGFP (SuperGlo, Qbiogene, Inc)ExpressionInsectMutationPromoterAvailable sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJB051 (mEos2-ZapA)
Plasmid#72650PurposeInducible expression of mEos2-ZapA in bacteriaDepositorInsertmEos2-ZapA
UseTagsmEos2ExpressionBacterialMutationPromoterT5-lacAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL3-RpS12-Gateway
Plasmid#71508PurposeflySTARR-seq validation vector with Gateway cassette downstream of the firefly luciferase gene and the RpS12 core promoterDepositorTypeEmpty backboneUseFlystarr-seq validation vectorTagsluciferaseExpressionInsectMutationPromoterAvailable sinceDec. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSTARR-seq_fly-eEF1delta
Plasmid#71501PurposeflySTARR-seq screening vector with eEF1delta core promoterDepositorTypeEmpty backboneUseFlystarr-seq screening vectorTagssgGFP (SuperGlo, Qbiogene, Inc)ExpressionInsectMutationPromoterAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJBL-RNAINS4
Plasmid#69456PurposeRNA-IN S4 variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)DepositorInsertRNA-IN S4 + SFGFP
UseSynthetic BiologyTagsExpressionBacterialMutationmutations for S4 variant (from wt)PromoterJ23119 and J23119 (sigma 70)Available sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJBL-RNAINS3
Plasmid#69455PurposeRNA-IN S3 variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)DepositorInsertRNA-IN S3
UseSynthetic BiologyTagsExpressionBacterialMutationmutations for S3 variant (from wt)PromoterJ23119 and J23119 (sigma 70)Available sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA5woH
Plasmid#65019PurposeExpresses the P94R/D160N/D165K/K190E/K196T mutant of Staphylococcus aureusDepositorInsertsortase A
UseTagsS tagExpressionBacterialMutationdeleted amino acids 1-59, P94R, D160N, D165A, K19…Promotertetracycline promoter/operatorAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA2woH
Plasmid#65018PurposeExpresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsS tagExpressionBacterialMutationdeleted amino acids 1-59, E105K, E108APromotertetracycline promoter/operatorAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only