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Showing: 4361 - 4380 of 5448 results

  1. pLCHKO_TK1_HPRT1_Lb_4

    Plasmid
    #155062
    Purpose
    Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and three intergenic sites & HPRT1 using LbCas12a (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA targeting TK1 using SpCas9 and three intergenic sites & HPRT1 using LbCas12a
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    155062-plasmid-map-sequence-id-308576

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  3. pKK-RNAi-nucCHERRYmiR-EGFP-TEV

    Plasmid
    #105810
    Purpose
    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with N-terminal EGFP.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    RNAi; Flp-in competent
    Tags
    EGFP-TEV
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    105810-plasmid-map-sequence-id-204854

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  4. pKK-RNAi-nucCHERRYmiR-TEV-EGFP

    Plasmid
    #105814
    Purpose
    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with C-terminal EGFP.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    RNAi; Flp-in competent
    Tags
    TEV-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105814-plasmid-map-sequence-id-203426

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  5. Tom20-mChF-AIP(TD)

    Plasmid
    #61521
    Purpose
    Expression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry, Tom20, and AIP with TD mutation
    Depositor
    Takanari Inoue
    Article
    Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:
    Insert
    FK506 binding protein 1A (FKBP1A Human)
    Use
    Tags
    AIP with TD mutation (see comments), Tom20, and m…
    Expression
    Mammalian
    Mutation
    TD mutation (see comments)
    Promoter
    CMV
    Available since
    April 17, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. pcDNA3.1-KozATG-myc-dL5-2XG4S-mCer3-SKL

    Plasmid
    #73210
    Purpose
    Expresses myc-dL5(E52D)-mCer3 fusion protein in peroxisomes of mammalian cells with a C-terminal PTS (SKL). (MBIC5, dL5**, FAP)
    Depositor
    Marcel Bruchez
    Article
    Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46
    Insert
    KozATG-myc-dL5-2XG4S-mCer3-SKL (MYC Human, Synthetic)
    Use
    Tags
    The FAP and mCerulean3 are fused with 2 copies of…
    Expression
    Mammalian
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    CMV
    Available since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    73210-plasmid-map-sequence-id-139746

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  7. mChF-AIP (TD)

    Plasmid
    #61529
    Purpose
    Expression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIP (with TD mutation)
    Depositor
    Takanari Inoue
    Article
    Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:
    Insert
    FK506 binding protein 1A (FKBP1A Human)
    Use
    Tags
    AIP with TD mutation (see comments) and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 1, 2015
    Availability
    Academic Institutions and Nonprofits only

  8. mChF-AIP (TE)

    Plasmid
    #61530
    Purpose
    Expression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIP (with TE mutation)
    Depositor
    Takanari Inoue
    Article
    Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:
    Insert
    FK506 binding protein 1A (FKBP1A Human)
    Use
    Tags
    AIP with TE mutation (see comments) and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 17, 2015
    Availability
    Academic Institutions and Nonprofits only

  10. CCM2(413-438)_pHisSUMO(K0)

    Plasmid
    #224065
    Purpose
    Bacterial expression of the C-terminal helix of CCM2 (residues 413-438) fused to an N-terminal hexa-histidine tag and SUMO(K0) [all lysines in SUMO mutated to arginines]. HRV-3C cleavage site
    Depositor
    Ben Goult
    Article
    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003
    Insert
    ccm2 (CCM2 Human)
    Use
    Tags
    6xHIS; SUMO(K0)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    224065-plasmid-map-sequence-id-443082

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  11. PLL5.0-Anillin ShRNA-Cherry-Anillin deltaAnillin homology domain

    Plasmid
    #187277
    Purpose
    Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin delta Anillin homology domain
    Depositor
    Alpha Yap
    Article
    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.
    Insert
    Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mutation
    deltaAnillin homology domain
    Promoter
    U6, CMV
    Available since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    187277-plasmid-map-sequence-id-433886

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  12. pETDUET-GST AO7RE ∆238-245

    Plasmid
    #139318
    Purpose
    GST tagged AO7 encoding aa 126-258 with an internal deletion that results in loss of E2 binding for expression in bacteria
    Depositor
    Allan Weissman
    Article
    Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3
    Insert
    AO7 aa 126-258 (RNF25 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    AO7 cloned into the first multiple cloning site o…
    Promoter
    T7 promoter-1
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    139318-plasmid-map-sequence-id-275576

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  13. pCEP4-myc-EFNB2 (D62Q-Q130L-V167L)

    Plasmid
    #200977
    Purpose
    Mammalian expression plasmid for myc-tagged EFNB2 (D62Q-Q130L-V167L specificity mutant)
    Depositor
    Erik Procko
    Article
    Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do
    Insert
    EFNB2 (EFNB2 Human)
    Use
    Tags
    Extracellular c-myc epitope tag and Signal peptid…
    Expression
    Mammalian
    Mutation
    D62Q-Q130L-V167L (increase specificity towards he…
    Promoter
    CMV
    Available since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    200977-plasmid-map-sequence-id-397620

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  17. pET-hMICU1EF

    Plasmid
    #199180
    Purpose
    This plasmid is used to express a EF-hand disabled human MICU1 protein in E. coli. The protein is fused with a maltose binding protein in the N-terminus, and also contains a C-terminal His6 tag.
    Depositor
    Ming-Feng Tsai
    Article
    Tsai et al Proc Natl Acad Sci U S A. 2023 Apr 18;12
    Insert
    MICU1 (MICU1 Human)
    Use
    Tags
    His6 and MBP, Thrombin site, TEV site
    Expression
    Mutation
    D421A, E432K, F433W, F453W
    Promoter
    Available since
    Aug. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    199180-plasmid-map-sequence-id-403144

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  18. TFORF0973

    Plasmid
    #143252
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF124 (ZNF124 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    143252-plasmid-map-sequence-id-396118

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  19. TFORF0393

    Plasmid
    #142612
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF7 (ZNF7 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142612-plasmid-map-sequence-id-396003

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  20. TFORF0970

    Plasmid
    #141771
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF124 (ZNF124 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141771-plasmid-map-sequence-id-415101

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Showing: 4361 - 4380 of 5448 results