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C239-E03: attB2r-IRES-ffLuc2-pA-attB3

Plasmid

#162919

Purpose

Gateway attB2r/attB3 entry clone for generation of polycistronic ffLuc2 reporter using an internal ribosome entry sequence (IRES)

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

firefly luciferase with upstream IRES sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pONL-N1_LAMP1

Plasmid

#65686

Purpose

Expresses LAMP1-ONL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

LAMP1

Use

Luciferase

Tags

ONL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only
pONL-C1_H2B

Plasmid

#65704

Purpose

Expresses ONL-H2B in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

histone H2B

Use

Luciferase

Tags

ONL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 15, 2015

Availability

Academic Institutions and Nonprofits only

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pJOG658

Plasmid

#105409

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

3xHA tag fused to Luciferase CDS

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI/ BpiI restriction sites removed

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pONL-N1_PTS2

Plasmid

#65677

Purpose

Expresses PTS2-ONL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

peroxisomal targeting signal 2

Use

Luciferase

Tags

ONL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pCNL-C1_H2B

Plasmid

#65703

Purpose

Expresses CNL-H2B in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

histone H2B

Use

Luciferase

Tags

CNL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 15, 2015

Availability

Academic Institutions and Nonprofits only

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pSTARR-seq_fly-pnr

Plasmid

#71503

Purpose

flySTARR-seq screening vector with pnr core promoter

Depositor

Alexander Stark

Article

Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

Type

Empty backbone

Use

Flystarr-seq screening vector

Tags

sgGFP (SuperGlo, Qbiogene, Inc)

Expression

Insect

Mutation

Promoter

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pSTARR-seq_fly-NipB

Plasmid

#71502

Purpose

flySTARR-seq screening vector with NipB core promoter

Depositor

Alexander Stark

Article

Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

Type

Empty backbone

Use

Flystarr-seq screening vector

Tags

sgGFP (SuperGlo, Qbiogene, Inc)

Expression

Insect

Mutation

Promoter

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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p252 pPr-CREM/CMV-STOP-luc

Plasmid

#8406

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

CREM/lox/luciferase

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

modified Cre

Promoter

Available since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pLEX_970_puro_DEST_V5_LUC

Plasmid

#45290

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

Luciferase

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2013

Availability

Academic Institutions and Nonprofits only
pSMART-HCKan-yibDp-VHH72

Plasmid

#202467

Purpose

Expresses the nanobody VHH72 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody VHH72

Use

Tags

6X-His

Expression

Bacterial

Mutation

Promoter

yibDp

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-H6antivenin

Plasmid

#202470

Purpose

Expresses the nanobody H6 antivenin after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody H6 antivenin

Use

Tags

6X-His

Expression

Bacterial

Mutation

Promoter

yibDp

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-VCAM1

Plasmid

#202471

Purpose

Expresses the nanobody VCAM1 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody VCAM1

Use

Tags

6X-His

Expression

Bacterial

Mutation

Promoter

yibDp

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSMART-HCKan-yibDp-VHH-1B5

Plasmid

#202472

Purpose

Expresses the nanobody 1B5 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody 1B5

Use

Tags

6X-His

Expression

Bacterial

Mutation

Promoter

yibDp

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSMART-HCKan-yibDp-VHH-2E7

Plasmid

#202473

Purpose

Expresses the nanobody 2E7 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody 2E7

Use

Tags

6X-His

Expression

Bacterial

Mutation

Promoter

yibDp

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSMART-HCKan-yibDp-VHH-3E3

Plasmid

#202474

Purpose

Expresses the nanobody 3E3 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody 3E3

Use

Tags

6X-His

Expression

Bacterial

Mutation

Promoter

yibDp

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSMART-HCKan-yibDp-VHH-C9BoNT-A

Plasmid

#202475

Purpose

Expresses the nanobody C9 BoNT-A after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody C9 BoNT/A

Use

Tags

6X-His

Expression

Bacterial

Mutation

Promoter

yibDp

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSMART-HCKan-yibDp-VHH-E7BoNT-A

Plasmid

#202476

Purpose

Expresses the nanobody E7 BoNT-A after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody E7 BoNT/A

Use

Tags

6X-His

Expression

Bacterial

Mutation

Promoter

yibDp

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSMART-HCKan-yibDp-VHH-JM3

Plasmid

#202477

Purpose

Expresses the nanobody JM3 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody JM3

Use

Tags

6X-His

Expression

Bacterial

Mutation

Promoter

yibDp

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSMART-HCKan-yibDp-VHH-J3

Plasmid

#202478

Purpose

Expresses the nanobody J3 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody J3

Use

Tags

6X-His

Expression

Bacterial

Mutation

Promoter

yibDp

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge