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Showing: 10851 - 10900 of 14292 results

  1. pCiPS-NIFV

    Plasmid
    #110807
    Purpose
    Non-Integrating Foamy Virus pol-expressing helper plasmid with mutations in the DDE catalyic core motif of the integrase sequence, remaining as unintegated episomes in transduced cells
    Depositor
    David Russell
    Article
    Deyle et al J Virol. 2010 Sep;84(18):9341-9. doi: 10
    Insert
    Pol
    Use
    Tags
    Expression
    Mammalian
    Mutation
    2 mutations in the DDE catalyic core motif of the…
    Promoter
    CMV
    Available since
    June 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    110807-plasmid-map-sequence-id-222708

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  21. pUBC_HA_2xPP7-VP64

    Plasmid
    #68418
    Purpose
    Transient expression of two tandem copies of the non-aggregating delta-FG mutant, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoter
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    PP7(deltaFG)
    Use
    Synthetic Biology
    Tags
    HA and VP64
    Expression
    Mammalian
    Mutation
    PP7: [delta]67_74 (non-aggregating)
    Promoter
    human UbC
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68418-plasmid-map-sequence-id-125550

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  26. p123shy1-mCherry-HA

    Plasmid
    #67875
    Purpose
    encodes shy1 with a C-terminal mCherry-HA tag under control of its endogenous promoter.It's suitable for localization studies in Ustilago maydis.
    Depositor
    Armin Djamei
    Article
    Rabe et al Mol Microbiol. 2013 Jul;89(1):179-88. do
    Insert
    shy1
    Use
    Expression in ustilago maydis
    Tags
    HA and mCherry
    Expression
    Mutation
    Promoter
    shy1
    Available since
    Aug. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    67875-plasmid-map-sequence-id-121040

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  33. pTd-4-15-13-10

    Plasmid
    #63835
    Purpose
    This plasmid is designed to validate the biophysical model of translational coupling
    Depositor
    Howard Salis
    Article
    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71
    Insert
    CFP, mRFP1 and GFPmut3b
    Use
    Tags
    Expression
    Bacterial
    Mutation
    The intergenic distance between each gene is -4 n…
    Promoter
    J23100
    Available since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  36. pU6_(GLuc)_INT[Spinach2]

    Plasmid
    #68430
    Purpose
    Transient expression in mammalian cells of an "INT" construct_bearing the "Spinach2" aptamer, targeting the GLuc reporter. U6 promoter
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    INT construct_bearing the "Spinach2" aptamer
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68430-plasmid-map-sequence-id-120208

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  46. pCMV/MASC_(GLuc)_INT

    Plasmid
    #68440
    Purpose
    Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/ MASC expression backbone.
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    INT construct_bearing three PP7 Stem-loops
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    68440-plasmid-map-sequence-id-124660

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  50. pDC146

    Plasmid
    #169366
    Purpose
    Expresses Syntaxin1-265 C145S, S249C, K253C ; SNAP25 ; 6xHis-TEV-Synaptobrevin1-96
    Depositor
    Axel Brunger
    Article
    Cipriano et al J Biol Chem. 2013 Aug 9;288(32):23436-45
    Insert
    Syntaxin1-265 C145S, S249C, K253C ; SNAP25 ; 6xHis-TEV-Synaptobrevin1-96
    Use
    Tags
    6 his tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    169366-plasmid-map-sequence-id-333023

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Showing: 10851 - 10900 of 14292 results