pET-22b(+)-HpENR
(Plasmid
#12651)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 12651 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET-22b(+)
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5493
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namenoyl-acyl carrier protein reductase
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Alt nameENR
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SpeciesHelicobacter pylori
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Insert Size (bp)825
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GenBank IDAAD05765
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Entrez GenefabI
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-22b(+)-HpENR was a gift from SeWon Suh (Addgene plasmid # 12651 ; http://n2t.net/addgene:12651 ; RRID:Addgene_12651) -
For your References section:
Crystallization and preliminary X-ray crystallographic analysis of enoyl-acyl carrier protein reductase from Helicobacter pylori. Lee HH, Yun J, Moon J, Han BW, Lee BI, Lee JY, Suh SW. Acta Crystallogr D Biol Crystallogr. 2002 Jun . 58(Pt 6 Pt 2):1071-3. 10.1107/S0907444902007151 PubMed 12037321