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Addgene

pET-22b(+)-HpENR
(Plasmid #12651)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12651 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET-22b(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5493
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    noyl-acyl carrier protein reductase
  • Alt name
    ENR
  • Species
    Helicobacter pylori
  • Insert Size (bp)
    825
  • GenBank ID
    AAD05765
  • Entrez Gene
    fabI

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-22b(+)-HpENR was a gift from SeWon Suh (Addgene plasmid # 12651 ; http://n2t.net/addgene:12651 ; RRID:Addgene_12651)
  • For your References section:

    Crystallization and preliminary X-ray crystallographic analysis of enoyl-acyl carrier protein reductase from Helicobacter pylori. Lee HH, Yun J, Moon J, Han BW, Lee BI, Lee JY, Suh SW. Acta Crystallogr D Biol Crystallogr. 2002 Jun . 58(Pt 6 Pt 2):1071-3. 10.1107/S0907444902007151 PubMed 12037321