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Plasmid#74821PurposeGateway cloning compatible binary vector for N-terminal fusion with T7 (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceSept. 29, 2016AvailabilityAcademic Institutions and Nonprofits only
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pGWB426
Plasmid#74820PurposeGateway cloning compatible binary vector for C-terminal fusion with T7 (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
T/brachyury(2A-Dre)
Plasmid#140761PurposeTargeting vector to T/brachyury gene locus for induction of P2A-fused Dre, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F3) sequence.DepositorInsertDre
UseMouse TargetingTagsExpressionMutationPromoterEndogenous T/brachyuryAvailable sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJH2827
Plasmid#179542PurposePnmr-1::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans AVA/E/D premotor IN and others expression of miniSOG-SL2 RFPDepositorInserttomm20::miniSOG (tomm-20 Nematode)
UseTagswCherryExpressionWormMutationPromoterPnmr-1Available sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shPrc1
Plasmid#160959PurposePrc1 shRNA in pMKO.1 retroviral vectorDepositorInsertPrc1 shRNA (Prc1 Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterU6Available sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_Malat1_E
Plasmid#72624PurposeExpresses two gRNAs targeting MALAT1 promoterDepositorInsertgRNAs toward Malat1
UseTagsExpressionMammalianMutationPromoterU6 (gRNA1) and H1 (gRNA2)Available sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSADdeltaG-GFP-AlstR
Plasmid#32647DepositorInsertsUseRabies virusTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
sgRNA4_MYOD1
Plasmid#64139PurposePhotoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassetteDepositorInsertsgRNA1_MYOD1 (MYOD1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
spa-GCaMP6f
Plasmid#67558Purposephotoactivatable fluorescent calcium reporterDepositorInsertspa-GCaMP6f
UseTagsHIS-tagExpressionMammalianMutationK65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…PromoterCMVAvailable sinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
XW20
Plasmid#65836PurposePunc-4-QF-3'UTR-unc-54 (The endogenous SphI, KpnI and SacI sites in QF are removed, and no SL2-mcherry)DepositorInsertOR74A quinic acid utilization activator (qa-1F)
UseTagsExpressionWormMutationThe endogenous SphI, KpnI and SacI sites in QF ar…PromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-DIO-mAPX
Plasmid#79908PurposeAdeno-associated virus plasmid, Cre-dependent FLEX switch, mAPXDepositorInsertmAPX
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
SaABE8e-dimer
Plasmid#138501PurposeA-to-G base editorDepositorInsertecTadA(WT)-ecTadA(8e)-nSaCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hSyntenin2b
Plasmid#89437Purposemammalian expression of EGFP tagged human Syntenin2bDepositorInsertSDCBP2 (SDCBP2 Human)
UseTagsEGFPExpressionMammalianMutation*see belowPromoterCMVAvailable sinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
sspa-GCaMP6f
Plasmid#67561Purposeshort photoactivatable fluorescent calcium reporterDepositorInsertsspa-GCaMP6f
UseTagsExpressionMammalianMutationK65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…PromoterAvailable sinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN(E12,14K)-mPAGFP
Plasmid#168495PurposeMammalian expression of the E12,14K mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertE12,14K mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Barcode backbone
Plasmid#158665PurposeThis plasmid serves as the backbone for the visual barcodes described in our paper. It has a CMV-Puro-F2A backbone to which we insert a fluorescent protein cellular localization combinationDepositorTypeEmpty backboneUseLentiviralTagshcREDExpressionMammalianMutationPromoterAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH3409
Plasmid#179574PurposePceh-12::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans VB-MN -specific expression of miniSOG-SL2 RFPDepositorInserttomm20::miniSOG (tomm-20 Nematode)
UseTagswCherryExpressionWormMutationPromoterPceh-12Available sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
enAsABE8e-dimer
Plasmid#138507PurposeA-to-G base editorDepositorInsertecTadA(WT)-ecTadA(8e)-dAsCas12a (En)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_UCA1
Plasmid#72628PurposeExpresses two gRNAs targeting the UCA1 promoterDepositorInsertgRNAs toward UCA1
UseTagsExpressionMammalianMutationPromoterU6 (gRNA1) and H1 (gRNA2)Available sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
MDH1-PGK-FLAG-TAT-86-A
Plasmid#35977DepositorInserttransactivating protein (tat Human Immunodeficiency Virus)
UseRNAi and RetroviralTagsFlagExpressionMammalianMutationFrom the "A" HIV cladePromoterAvailable sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pORANGE Cacnb3-GFP KI
Plasmid#139662PurposeEndogenous tagging of CaVβ3: C-terminal (amino acid position: W479)DepositorInsertgRNA and GFP donor
UseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUWR-CadTS-C
Plasmid#58326PurposeDestination vector for inserting ubi-CadTS control to Drosophila melanogaster genomeDepositorInsertE-cadherin tension sensor control
UseTagsExpressionInsectMutationPromoterpoly-ubiquitinAvailable sinceAug. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUWR-CadTS
Plasmid#58325PurposeDestination vector for inserting ubi-CadTS to Drosophila melanogaster genomeDepositorInsertE-cadherin tension sensor
UseTagsExpressionInsectMutationPromoterpoly-ubiquitinAvailable sinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV mTurquoise2-shadowY tandem
Plasmid#157775PurposeProduces a fusion between mTurquoise2 and shadowY. It can be used as a positive control for FRET (using FLIM)DepositorTypeEmpty backboneUseTagsmTurquoise2 and shadowYExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
ShuttleMCP
Plasmid#50959PurposeA backbone for adenovirus creation by the AdEasy method, similar to ShuttleCMV (Addgene Plasmid # 16403), except it uses a mouse cofilin promoter (MCP) instead of the CMV promoterDepositorTypeEmpty backboneUseAdenoviralTagsExpressionMammalianMutationPromoteruses portion of the mouse cofilin gene as a mouse…Available sinceFeb. 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLib6.4-Aalb_726
Plasmid#176661PurposeExpression of sgRNA under Ae. albopictus U6 (AALF029726) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprTagsExpressionInsectMutationPromoterAvailable sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pME18S-F-R-hb2AR
Plasmid#149367PurposeExpresses human b2AR in mammalian cells.DepositorInsertb2AR (ADRB2 Human)
UseTagsFLAG and rhodopsinExpressionMammalianMutationPromoterSR alphaAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4-Aaeg_763
Plasmid#176658PurposeExpression of sgRNA under Ae. Aegypti U6 (AAEL017763) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprTagsExpressionInsectMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSCV-ERK5-AEF
Plasmid#31816DepositorInsertErk5 (Mapk7 Mouse)
UseRetroviralTagsExpressionMammalianMutationT219A and Y221FPromoterPCMV-LTRAvailable sinceSept. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGWB422
Plasmid#74816PurposeGateway cloning compatible binary vector for C-terminal fusion with GST (no promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWB428
Plasmid#74822PurposeGateway cloning compatible binary vector for C-terminal fusion with TAP (no promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hHDAC4
Plasmid#21096DepositorInsertHistone Deacetylase 4 (HDAC4 Human)
UseTagsExpressionMammalianMutationshRNA target sequence was mutated (mutated nucleo…PromoterCAGAvailable sinceMay 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLpC-Zipp.-L-IFP-F[2]+Venus (mammalian)
Plasmid#52906PurposeBinary retroviral pl. (puromycin). Zipper (PacI/ClaI):Linker (GGGGS)X2+IFP-F[2]. In the second MCS, the Venus fluorescent protein is cloned in between PmeI/SalI.Confers ampicillin resistance to DH5α.DepositorInsertZipp.-L-IFP-F[2]+Venus
UseRetroviralTagsL-IFP-F[2]ExpressionMammalianMutationPromoterUnknownAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLpC-Zipp.-L-IFP-F[1]+Plum (mammalian)
Plasmid#52907PurposeBinary retroviral pl. (puromycin). Zipper (PacI/ClaI):Linker (GGGGS)X2+IFP-F[1]. In the second MCS, the Plum fluorescent protein is cloned in between PmeI/SalI. Confers ampicillin resistance to DH5α.DepositorInsertZipp.-L-IFP-F[1]+Plum
UseRetroviralTagsL-IFP-F[1]ExpressionMammalianMutationPromoterUnknownAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
sgRNA3_MYOD1
Plasmid#64138PurposePhotoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassetteDepositorInsertsgRNA1_MYOD1 (MYOD1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-CXCR4
Plasmid#42052DepositorInsertCXCR4 shRNA (CXCR4 )
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
CALNL-DCX-eGFP
Plasmid#28310DepositorInsertDoublecortin (Dcx Rat)
UseCre/LoxTagseGFPExpressionMammalianMutationPromoterAvailable sinceApril 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTYF-super-mfPENK-2.2k-Venus-WPRE
Plasmid#156400PurposeExpresses Venus under PENK gene promoterDepositorInsertsVenus
Gal4p65
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pISH-Dlx3
Plasmid#74342PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Dlx3. Not for gene expression.DepositorInsertdistal-less homeobox 3 (Dlx3 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pISH-Lhx2
Plasmid#74337PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Lhx2. Not for gene expression.DepositorInsertLIM homeobox protein 2 (Lhx2 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJAG98-mTurquoise2-FHA2-AURKB substrate-YPet
Plasmid#83286Purposeuntargeted Aurora B FRET sensor with mTurquoise2 and YPet FRET pairDepositorInsertmTurquoise2-FHA2-AURKB substrate-YPet
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBABE HSF Flag Ac
Plasmid#1949DepositorInsertHeat Shock Factor 1 (HSF1 Human)
UseRetroviralTagsflagExpressionMammalianMutationAcidic mutations (D416, E493, E496 A)PromoterAvailable sinceMarch 23, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Plxnb2-ISH-2kb
Plasmid#68036PurposeContains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouseDepositorInsertPlexin-B2 (Plxnb2 Mouse)
UseMrna in situ hybridization probeTagsExpressionMutationPromoterAvailable sinceAug. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Sema4c-ISH-2kb
Plasmid#68031PurposeContains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouseDepositorInsertSemaphorin-4C (Sema4c Mouse)
UseMrna in situ hybridization probeTagsExpressionMutationPromoterAvailable sinceAug. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
STAGR_SAMScaffold_h7SK
Plasmid#102846PurposeCan be used as PCR template for a STAgR reactionDepositorInsertSTAgR Insert SAM_h7SK
UsePcr template for stagr insertsTagsExpressionMutationPromoterH7SKAvailable sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pME18S-3HA-mULK2(K39T)
Plasmid#22899DepositorInsertUNC-51-like kinase 2 (Ulk2 Mouse)
UseTags3xHAExpressionMammalianMutationchanged Lysine 39 to ThreoninePromoterAvailable sinceSept. 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTYF-super-mfSERT-1.9k-Venus-WPRE
Plasmid#156394PurposeExpresses Venus under SERT gene promoterDepositorInsertsVenus
Gal4p65
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET-spa-GCaMP6f
Plasmid#67563Purposebacterial expression of a photoactivatable fluorescent calcium reporterDepositorInsertspa-GCaMP6f
UseTagsHIS-tagExpressionBacterialMutationK65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…PromoterAvailable sinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hNPPC-RFP
Plasmid#22921DepositorInserthuman c-type natruiretic peptide precursor promoter driving DsRedExpress (NPPC Human)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2010AvailabilityAcademic Institutions and Nonprofits only